| Roots & Bloom CIC | Agriculture & fishing | 2019 |
| Roots Ahead Ltd | Professional & scientific | 2024 |
| Roots Mbili Arts CIC | Education | 2025 |
| Roots Mbili Theatre Ltd | Arts & recreation | 2021 |
| Rope & Rotor Inspection Services Ltd | Professional & scientific | 2025 |
| Rope Access Magh Ltd | Construction | 2021 |
| Rope Access Services Limited | Construction | 2006 |
| Rope Rigging Ltd | Construction | 2026 |
| Rope Solutions Ltd | Administrative & support | 2021 |
| Rope Wind Ltd | Other services | 2021 |
| Ropesafe Holdings Ltd | Financial services | 2026 |
| Ropesafe Ltd | Construction | 2021 |
| Roqueburn Investments Limited | Real estate | 2006 |
| Rosamond Court Maintenance(Bradway)Limited | Households | 1966 |
| Rosary Bead Care Limited | Health & social care | 2013 |
| Rosary Estates Limited | Real estate | 2020 |
| Rosava Capital Holding Ltd | Mining & quarrying | 2025 |
| Rosco Ware Limited | Wholesale & retail | 2022 |
| Roscoe Development Management Limited | Professional & scientific | 2002 |
| Rose Alqurashi Ltd | Manufacturing | 2023 |
| Rose Cottage Care Holdings Limited | Financial services | 2026 |
| Rose Cottage Farm Estates Ltd | Agriculture & fishing | 2024 |
| Rose Cottage Nursing Care Limited | Health & social care | 1995 |
| Rose Expedition Ltd | Transport & logistics | 2024 |
| Rose Gold Hair Limited | Other services | 2018 |
| Rose Harper Estates Limited | Real estate | 2011 |
| Rose Lock Property Ltd | Real estate | 2025 |
| Rose Lodge Small Animal Boarding Ltd | Agriculture & fishing | 2019 |
| Rose Moore Hospitality Limited | Hospitality | 2025 |
| Rose Poker Ltd | Arts & recreation | 2020 |
| Rose Support & Wellbeing Services Ltd | Administrative & support | 2025 |
| Roseacre Freeholds Limited | Hospitality | 2024 |
| Roseacre PUB Company Ltd | Hospitality | 2014 |
| Rosedale Garage Limited | Wholesale & retail | 2026 |
| Rosedene Farm Ltd | Agriculture & fishing | 2016 |
| Roses ARE Blue Ltd | Other services | 2026 |
| Roseside Health Care Limited | Health & social care | 2022 |
| Rosesmulti-Services Ltd | Construction | 2023 |
| Rosewalk Fairleigh Ltd | Wholesale & retail | 2022 |
| Rosewalk Herries Ltd | Wholesale & retail | 2022 |
| Rosewalk Teynham Ltd | Wholesale & retail | 2022 |
| Rosewood Injury Claims Limited | Financial services | 2007 |
| Rosewood Loft Creations Ltd | Construction | 2011 |
| Rosewoods Plastering Projects Limited | Construction | 2020 |
| Roshnee Wedding Services Ltd | Hospitality | 2016 |
| Roshni Sheffield Asian Women's Resource Centre | Hospitality | 2012 |
| Rosie Care Home Ltd | Professional & scientific | 2015 |
| Rosie Hodge Books Limited | Professional & scientific | 2004 |
| Rosie's Craft Shop Ltd | Wholesale & retail | 2012 |
| Roslyn Mews Cattery Limited | Other services | 2009 |
| Roslyn's Accounting Company Limited | Professional & scientific | 2008 |
| Roslyn's Property Limited | Real estate | 2023 |
| Ross and Liblanc Ltd | Construction | 2022 |
| Ross Andrew Properties Ltd | Real estate | 2023 |
| Ross Building Limited | Construction | 2024 |
| Ross Holdings (EOT) Limited | Financial services | 2025 |
| Ross Holdings Limited | Professional & scientific | 1994 |
| Ross Napier Coaching and Education Limited | Education | 2012 |
| Ross Plummer LLP | | 2021 |
| Ross Street Developments Limited | Real estate | 2020 |
| Ross's Barbers Ltd | Other services | 2020 |
| Ross's Barbers Retail Ltd | Other services | 2020 |
| Rossceuticals Ltd | Professional & scientific | 2021 |
| Rostate Limited | Health & social care | 2026 |
| Rostek UK Ltd | Construction | 2010 |
| Rostend Limited | Financial services | 1983 |
| Rostra Beauty Ltd | Health & social care | 2017 |
| Rostra Healthcare Ltd | Health & social care | 2007 |
| Rostra Medical Ltd | Wholesale & retail | 2015 |
| Rostra Property Ltd | Real estate | 2016 |
| Rostra UK Ltd | Health & social care | 2005 |
| Roszko Property Limited | Real estate | 2021 |
| Rotabroach Limited | Extraterritorial | 1995 |
| Rotamag Ltd | Professional & scientific | 2024 |
| Rotamag Rail Ltd | Professional & scientific | 2024 |
| Rotary Developments Limited | Construction | 1999 |
| Rotary Electrical Services Ltd | Manufacturing | 2000 |
| Rotary Engineering UK Limited | Manufacturing | 2003 |
| Rotary Holdings Ltd | Financial services | 2023 |
| Rotary Property Company Ltd | Real estate | 2023 |
| Rotation Pattern Limited | Other services | 1996 |
| Rother Broadcasting Limited | Information & communication | 2020 |
| Rother FM Limited | Information & communication | 2024 |
| Rother Medicals Ltd | Health & social care | 2017 |
| Rother Vale Consultancy Limited | Information & communication | 2025 |
| Rother Valley Cars Ltd | Wholesale & retail | 2014 |
| Rother Valley Manco Ltd | Real estate | 2023 |
| Rother Valley Optics Limited | Wholesale & retail | 2003 |
| Rother Valley Salvage Limited | Transport & logistics | 2007 |
| Rother Valley South PCN Limited | Administrative & support | 2023 |
| Rotherham Athletic Company Limited(The) | Real estate | 1905 |
| Rotherham Broadcasting CIC | Information & communication | 2020 |
| Rotherham Building Plastics Ltd | Wholesale & retail | 2016 |
| Rotherham Calibration Service Limited | Professional & scientific | 1998 |
| Rotherham CNC Limited | Manufacturing | 1998 |
| Rotherham Motor Company Limited | Extraterritorial | 2009 |
| Rotherham Sailing Club | Education | 2017 |
| Rothersec Ltd | Administrative & support | 2025 |
| Rotherside Developments Limited | Construction | 2020 |
| Rotor Clip Limited | Manufacturing | 1999 |