| AE Delivery Solutions Limited | Transport & logistics | 2023 |
| AE Dental Care Limited | Health & social care | 2026 |
| AE Electrical Contractors Limited | Construction | 2014 |
| AE Premier Holdings | Financial services | 2020 |
| AE Pubs Ltd | Hospitality | 2026 |
| AE Security Services Limited | Administrative & support | 2015 |
| AEG Homes Limited | Real estate | 2024 |
| Aegan Store Ltd | Wholesale & retail | 2018 |
| Aegean Art Gallery Ltd | Professional & scientific | 2021 |
| Aegiq Ltd | Information & communication | 2019 |
| Aegis 24/7 Ltd | Health & social care | 2019 |
| Aegis Haven Support Ltd | Health & social care | 2025 |
| Aelys Group Limited | Wholesale & retail | 2020 |
| Aeni Limited | Information & communication | 2026 |
| AEO Consultancy Limited | Professional & scientific | 2024 |
| Aeon Business Services Limited | Administrative & support | 2012 |
| Aequate Ltd | Information & communication | 2026 |
| Aequum Bars Ltd | Hospitality | 2018 |
| Aerial Hoops Ltd | Wholesale & retail | 2020 |
| Aerialconnect Ltd | Information & communication | 2025 |
| Aerisurv Ltd | Professional & scientific | 2020 |
| Aerospace Ltd | Manufacturing | 2020 |
| Aerospider Cars AB Ltd | Wholesale & retail | 2025 |
| AES Medical Services Ltd | Health & social care | 2024 |
| AES Smart Trading Ltd | Real estate | 2025 |
| Aesaim Limited | Professional & scientific | 2013 |
| Aesthetic Arch FM Ltd | Extraterritorial | 2025 |
| Aesthetic Healthcare Ltd | Health & social care | 2020 |
| Aesthetic Image Ltd | Other services | 2024 |
| Aesthetic Loft Global Investment Ltd | Other services | 2025 |
| Aesthetic Supplies Limited | Wholesale & retail | 2021 |
| Aesthetics by EVE Ltd | Health & social care | 2021 |
| Aestheticsbyem Ltd | Health & social care | 2021 |
| Aesthetika Limited | Health & social care | 2004 |
| Aestus Limited | Information & communication | 2023 |
| AET Lettings Limited | Real estate | 2025 |
| Aeternum Consultancy Ltd | Information & communication | 2026 |
| Aethelburg Solutions Limited | Wholesale & retail | 2025 |
| Aewm Ltd | Health & social care | 2021 |
| AF Chempharm Limited | Manufacturing | 1998 |
| AF Real Estate Limited | Real estate | 2025 |
| AF Transformation Academy Ltd | Arts & recreation | 2019 |
| AFE Certification Limited | Professional & scientific | 2026 |
| Affas Ltd | Professional & scientific | 2025 |
| Affdal Ltd | Transport & logistics | 2026 |
| Affinity 2020 CIC | Education | 2020 |
| Affinity Circle Ltd | Other services | 2017 |
| Affinity Electrical Services Limited | Professional & scientific | 2011 |
| Affinity Four Ltd | Wholesale & retail | 2026 |
| Affinity Funeral Care Ltd | Financial services | 2021 |
| Affinity Intent Limited | Professional & scientific | 2020 |
| Affinity Investments Ltd | Administrative & support | 2022 |
| Affinity IT Services Limited | Information & communication | 2006 |
| Affinity One Limited | Wholesale & retail | 2020 |
| Affinity Property Solutions Limited | Real estate | 2016 |
| Afflatus Consulting Limited | Administrative & support | 2014 |
| Afflow Ltd | Professional & scientific | 2014 |
| Affluent Agency Ltd | Professional & scientific | 2019 |
| Affordable Cars Sheffield Limited | Wholesale & retail | 2019 |
| AFG Finest Nuts Limited | Wholesale & retail | 2024 |
| AFK Transport Ltd | Transport & logistics | 2017 |
| Afku Services Ltd | Health & social care | 2013 |
| Afnan Recruitment Ltd | Administrative & support | 2023 |
| AFP Vehicle Hire Ltd. | Wholesale & retail | 2011 |
| Africa Center for Artificial Intelligence CIC | Professional & scientific | 2025 |
| African and Caribbean Cancer Support Organisation CIC | Other services | 2025 |
| African Coffee Connect Limited | Administrative & support | 2023 |
| African Farmer's Delight Foods Ltd | Manufacturing | 2023 |
| African Higher Education and Research Observatory (Afrihero) | Education | 2013 |
| African Voices Platform C.I.C. | Information & communication | 2020 |
| Afro Beaute Ltd | Other services | 2025 |
| Afrovibes Sheffield Ltd | Arts & recreation | 2022 |
| After Dark Fireworks Ltd | Arts & recreation | 2013 |
| After the FOX Ltd | Professional & scientific | 2024 |
| Afterglow Event Hire Limited | Administrative & support | 2023 |
| Afterglow Movie Ltd | Information & communication | 2026 |
| Afterwood Ltd | Wholesale & retail | 2011 |
| Aftrak Ltd | Manufacturing | 2024 |
| AFW Property Limited | Real estate | 2024 |
| Afya Lafia Innovations Ltd | Manufacturing | 2025 |
| Afzal Investment Management Ltd | Extraterritorial | 2014 |
| AG Aggregates & Haulage Ltd | Wholesale & retail | 2025 |
| AG Environmental Scaffolding Ltd | Construction | 2016 |
| AG Garrant Limited | Extraterritorial | 1973 |
| AG Group Limited | Manufacturing | 1981 |
| AG Hairstyling Services Limited | Other services | 2020 |
| AG Rentals (Yorkshire) Limited | Real estate | 1987 |
| AG UK Group Limited | Wholesale & retail | 2020 |
| Agahi Limited | Real estate | 2013 |
| Aganics Limited | Construction | 2010 |
| Agape & IVY Limited | Health & social care | 2025 |
| Agasaf .I Limited | Wholesale & retail | 2017 |
| Agden Stoves Limited | Construction | 2023 |
| AGE UK Sheffield | Health & social care | 2004 |
| AGE UK Sheffield Enterprises Limited | Wholesale & retail | 1989 |
| Agency for Culture and Change Management | Education | 1998 |
| Agent PR Limited | Other services | 2013 |
| Agent Public Relations Limited | Professional & scientific | 2013 |
| Ageya Limited | Manufacturing | 2024 |
| AGF Building Services Ltd | Construction | 2024 |