| Explners Ltd | Information & communication | 2023 |
| Explore Care Limited | Health & social care | 2023 |
| Expoplus UK Ltd | Wholesale & retail | 2025 |
| Export Packing & Crating Ltd | Manufacturing | 2025 |
| Express Aluminium Ltd | Manufacturing | 2011 |
| Express Catering Equipment and Fabrications Ltd | Manufacturing | 2017 |
| Express Global Products Ltd | Wholesale & retail | 2020 |
| Express Grinding Services Limited | Manufacturing | 2014 |
| Express Gyros Ltd | Hospitality | 2024 |
| Express Installations Limited | Construction | 2020 |
| Express IT Consultancy Limited | Information & communication | 2021 |
| Express Maintenance Ltd | Construction | 2025 |
| Express Paving Limited | Construction | 2016 |
| Express Produce Ltd | Arts & recreation | 2014 |
| Express Sheff Ltd | Transport & logistics | 2024 |
| Express Stainless Fabrications Ltd | Manufacturing | 2020 |
| Express Worktops Limited | Wholesale & retail | 2016 |
| Expression Games Development Ltd | Information & communication | 2022 |
| Expression Games Ltd | Information & communication | 2011 |
| Expro Global Ltd | Professional & scientific | 2017 |
| Extension Plans Sheffield Limited | Professional & scientific | 2023 |
| Extensive Diversity Limited | Wholesale & retail | 2024 |
| Extra Hannab Ltd | Information & communication | 2024 |
| Extra Mile Fitness Experience Ltd | Education | 2026 |
| Extra Mile Fitness Ltd | Arts & recreation | 2020 |
| Extra Peche Seafood Limited | Wholesale & retail | 2025 |
| Extra Time Sports Bar Ltd | Hospitality | 2021 |
| Extraction Games Limited | Information & communication | 2022 |
| Extranet Limited | Professional & scientific | 2001 |
| Extravagance Retail Limited | Wholesale & retail | 2011 |
| Extreme Joinery Limited | Construction | 2019 |
| Extreme Present Limited | Arts & recreation | 2015 |
| Exxcel Care Ltd | Health & social care | 2026 |
| Exxels Ltd | Wholesale & retail | 2022 |
| Exyo Design Ltd | Manufacturing | 2015 |
| Exzact Joinery Ltd | Administrative & support | 2017 |
| EY UP Inns Limited | Hospitality | 2024 |
| Ey'Up Flower Farm Ltd | Agriculture & fishing | 2024 |
| Eyced Ltd | Health & social care | 2010 |
| EYE Correction Centre Limited | Real estate | 2016 |
| EYE Correction Centre UK East Limited | Health & social care | 2019 |
| EYE on Security Systems Limited | Administrative & support | 2019 |
| Eye-I Systems Ltd | Information & communication | 2008 |
| Eyeco (Harveys) Ltd | Wholesale & retail | 2019 |
| Eyeconstruction Limited | Construction | 2012 |
| Eyelashes Limited | Information & communication | 2019 |
| Eyelea Ltd | Health & social care | 2022 |
| EYEWEAR-51 Limited | Wholesale & retail | 2018 |
| Eyexam Ltd | Health & social care | 2021 |
| Eyeye Works Limited | Wholesale & retail | 2016 |
| Eyla Ltd | Professional & scientific | 2020 |
| Eymas Estates Ltd | Real estate | 2020 |
| Eyre & Co Accountants Limited | Professional & scientific | 2019 |
| Eyre Estates Ltd | Real estate | 2018 |
| Eyreforce Removals & Logistics Ltd | Water & waste | 2023 |
| Ezar Ltd | Extraterritorial | 2024 |
| EZE Village Limited | Administrative & support | 2020 |
| EZR Automotive Ltd | Wholesale & retail | 2022 |
| Ezzy Solution Limited | Wholesale & retail | 2025 |
| Ezzz Investments Limited | Real estate | 2026 |
| F & D Ltd | Professional & scientific | 2021 |
| F & H Coffee Ltd | Hospitality | 2017 |
| F & J Properties Limited | Real estate | 2019 |
| F & M Barbers Limited | Other services | 2022 |
| F & N Property Ltd | Real estate | 2008 |
| F & S Medical Imaging Ltd | Health & social care | 2020 |
| F a Building and Maintenance Ltd | Construction | 2024 |
| F a Leisure Services Limited | Hospitality | 2015 |
| F and B Property Group Ltd | Real estate | 2026 |
| F Bacon Property Ltd | Real estate | 2025 |
| F Butcher and SON Limited | Other services | 2010 |
| F C R Ludlam Limited | Real estate | 1991 |
| F Capital Logistics Limited | Transport & logistics | 2025 |
| F Funk Limited | Manufacturing | 2005 |
| F Kemble Limited | Real estate | 2026 |
| F M Caine & Sons Limited | Agriculture & fishing | 2018 |
| F R and J M Glazebrook and SON Limited | Agriculture & fishing | 2004 |
| F Whitham Ltd | Construction | 2011 |
| F&A Ecofleet Leasing Ltd | Administrative & support | 2025 |
| F&B Beighton Hand Carwash Ltd | Wholesale & retail | 2021 |
| F&D Logistics Limited | Transport & logistics | 2024 |
| F&J Consultancy Ltd | Information & communication | 2023 |
| F&R Kids Ltd | Wholesale & retail | 2024 |
| F&R Retail Limited | Wholesale & retail | 2017 |
| F-Line Hospitality Limited | Hospitality | 2022 |
| F-Tang Music Limited | Information & communication | 2012 |
| F. & J. Harben Limited | Real estate | 1979 |
| F. J. Forbes Estates Limited | Real estate | 2026 |
| F. Murdoch Refractory Construction Limited | Manufacturing | 1978 |
| F. Whitham (Sheffield) Limited | Construction | 2005 |
| F.A. Morris (Sheffield) Limited | Manufacturing | 1955 |
| F.C. Trading (U.K.) Limited | Wholesale & retail | 1995 |
| F.E.S. Building Services Engineers Ltd | Construction | 2019 |
| F.H.P. Hire & Sales Ltd | Administrative & support | 2016 |
| F.I. Investments Limited | Wholesale & retail | 2015 |
| F.J.Brindley & Sons (Sheffield) Limited | Manufacturing | 1930 |
| F.L.D.A. Blades Limited | Extraterritorial | 1993 |
| F.M. Finnegan Consulting Engineers Limited | Professional & scientific | 2011 |
| F.Rogers and SON (Transport) Limited | Real estate | 1956 |
| F.W. Wallace Limited | Wholesale & retail | 1947 |