| SDK Training Limited | Education | 2015 |
| SDS Construction Services Ltd | Construction | 2025 |
| Sdtate Ltd | Hospitality | 2025 |
| Seascape Bloodstock Limited | Agriculture & fishing | 2021 |
| Sedgehill Decor Limited | Construction | 2010 |
| Seedcloud Advisory Limited | Information & communication | 2012 |
| Semley Auctioneers Limited | Wholesale & retail | 2004 |
| Serenova Hair Retreat Limited | Other services | 2026 |
| Sesc Eco Ltd | Professional & scientific | 2025 |
| Sesc Fire and Security Ltd | Administrative & support | 2025 |
| Sesc Renewables Ltd | Construction | 2022 |
| Sesc Renewables, Plumbing & Heating Ltd | Construction | 2023 |
| Sesc Solutions Ltd | Construction | 2011 |
| Seseit Limited | Information & communication | 2001 |
| Sewa Energy Ltd | Manufacturing | 2008 |
| Sewa Engineering Limited | Extraterritorial | 2011 |
| SFA Franchise Ltd | Administrative & support | 2026 |
| Sha.Fyi Limited | Information & communication | 2024 |
| Shaftesbury & District Carers Association | Health & social care | 2008 |
| Shaftesbury Accounting Services Ltd | Professional & scientific | 2011 |
| Shaftesbury Antiques Ltd | Wholesale & retail | 2023 |
| Shaftesbury Autobody Repair Ltd | Administrative & support | 2016 |
| Shaftesbury Car Link Community Interest Company | Transport & logistics | 2023 |
| Shaftesbury Chamber of Commerce CIC | Administrative & support | 2025 |
| Shaftesbury DRAINS24 - Your Local Drainage Experts Ltd | Water & waste | 2025 |
| Shaftesbury Football Club 1888 Ltd | Arts & recreation | 2016 |
| Shaftesbury Football Club 3G Limited | Arts & recreation | 2022 |
| Shaftesbury Fresh Minds CIC | Other services | 2023 |
| Shaftesbury Glass Limited | Construction | 2022 |
| Shaftesbury Mortgages Limited | Professional & scientific | 2023 |
| Shaftesbury Nails Ltd | Other services | 2021 |
| Shaftesbury Osteopathic Practice Limited | Health & social care | 2002 |
| Shaftesbury Planning & Project Management Ltd | Professional & scientific | 2019 |
| Shaftesbury Sports Club Limited | Arts & recreation | 2013 |
| Shaftesbury Submission Grappling Ltd | Arts & recreation | 2026 |
| Shaftesbury Tyres & Batteries Limited | Manufacturing | 2018 |
| Shaftesbury Tyres Limited | Manufacturing | 2018 |
| Shaftesbury View Management Company Ltd | Households | 2020 |
| Shaston Events Ltd | Arts & recreation | 2025 |
| Shaston Ltd | Real estate | 2025 |
| Shearstock Limited | Transport & logistics | 2017 |
| Sheng Express Ltd | Hospitality | 2014 |
| Shorts Green Courtyard Management Association Limited | Households | 2015 |
| Shrl Holdings Limited | Financial services | 2026 |
| Signrite Signs & Graphics.Co.Uk Ltd | Manufacturing | 2009 |
| Silent Classics Limited | Wholesale & retail | 2022 |
| Simali Stone Ltd | Wholesale & retail | 2010 |
| Simon MAN Trading Limited | Information & communication | 1997 |
| Simply Computing Ltd | Information & communication | 2018 |
| Simply Feline Ltd | Professional & scientific | 2024 |
| Simply Venison Ltd | Agriculture & fishing | 2014 |
| Site-Fi Ltd | Information & communication | 2018 |
| SIX Rainbows Holdings Limited | Real estate | 2015 |
| Skoidos Limited | Mining & quarrying | 2026 |
| Small Batch Roasting Ltd | Wholesale & retail | 2025 |
| Smart (Green ORE) Ltd | Real estate | 2021 |
| SO Your Own Limited | Manufacturing | 2024 |
| Solar Repair Centre Ltd | Construction | 2023 |
| Solar Technicians Ltd | Construction | 2025 |
| Somerset Hire Services Limited | Extraterritorial | 2019 |
| South Coast Autos Ltd | Wholesale & retail | 2025 |
| South West Small Business Support Limited | Professional & scientific | 2018 |
| Spice Odyssey Ltd | Extraterritorial | 2022 |
| Squaretec Ltd | Education | 2010 |
| ST. Denis Lodge Residential Home Limited | Health & social care | 2002 |
| Stanley Baxter Archives Limited | Information & communication | 2025 |
| Stayloft UK Ltd | Real estate | 2025 |
| Steadijess Ltd | Information & communication | 2024 |
| Steve M Distribution Limited | Transport & logistics | 2011 |
| Story Stew Limited | Information & communication | 2015 |
| Storyline Analytics Limited | Professional & scientific | 2025 |
| Stour Publishing and Perry Holdings Limited | Financial services | 2019 |
| Stour Publishing and Perry Limited | Manufacturing | 2019 |
| Strattons Carpets Limited | Wholesale & retail | 2016 |
| Sturgess & Sturgess Limited | Construction | 2014 |
| SUE Newman Limited | Information & communication | 2009 |
| Sun Kissed Tanning Salons Ltd | Other services | 2021 |
| Sun Kissed Tanning Wincanton Ltd | Other services | 2023 |
| Supersonic Science CIC | Education | 2016 |
| Swann Recruitment Limited | Administrative & support | 2003 |
| Swans Trust (Shaftesbury) | Other services | 1997 |
| Swift Rocket Films Ltd | Information & communication | 2020 |
| Sylvafield Limited | Information & communication | 2014 |
| T J C Building & Groundworks Ltd | Construction | 2021 |
| Tailored Metal Roofing Ltd | Construction | 2025 |
| Taking Action for Sexual Health CIC | Health & social care | 2020 |
| Talbot (Plumbing & Heating) Limited | Construction | 2009 |
| Talisman London Limited | Wholesale & retail | 2000 |
| Terrier Consulting Limited | Other services | 2018 |
| Thanes Marine Ltd | Manufacturing | 2021 |
| THE Brave and Determined Company Limited | Wholesale & retail | 2022 |
| THE Central Prefix Register Limited | Information & communication | 1997 |
| THE Flange Project Limited | Professional & scientific | 2015 |
| THE Gillingham & Shaftesbury Agricultural Society | Arts & recreation | 2003 |
| THE Institute of Clinical Research | Education | 2000 |
| THE Physical Performance Coach Limited | Education | 2020 |
| THE Physical Performance Hub Ltd | Health & social care | 2023 |
| THE Pink House Mustique Ltd | Wholesale & retail | 2011 |
| THE Springhead Trust Limited | Education | 2005 |
| THE Tallons Management Limited | Households | 2016 |