| THE Keysie Company Ltd | Manufacturing | 2025 |
| THE Profit Rocket Ltd | Professional & scientific | 2019 |
| THE Smile LAB Glasgow Limited | Health & social care | 2019 |
| THE Souter Recruiter Limited | Administrative & support | 2026 |
| THE Strength Mill Ltd | Arts & recreation | 2025 |
| Thorburn Dean Holdings Ltd | Health & social care | 2026 |
| Thornylee Farms | | 1990 |
| Tibbie's in Selkirk Ltd | Hospitality | 2024 |
| Tidy Consultancy Ltd | Professional & scientific | 2022 |
| TMS Healthcare Limited | Health & social care | 2014 |
| Tonys Tower Ltd | Hospitality | 2025 |
| Toun Cryer Franchising Ltd | Information & communication | 2014 |
| Transform Arts CIC | Other services | 2019 |
| Triathlon Edinburgh Limited | Arts & recreation | 2015 |
| True North 1 Limited | Construction | 2024 |
| TT Dental Ltd | Health & social care | 2023 |
| Tuckwood Consulting Ltd | Professional & scientific | 2011 |
| Turfplus Limited | Administrative & support | 2016 |
| Turning Tides Scotland Ltd | Professional & scientific | 2014 |
| Tweed Valley Cycles Limited | Wholesale & retail | 2015 |
| Tweedmount Homes Ltd | Construction | 2024 |
| Tweedside Financial Services Ltd | Financial services | 2016 |
| Unicorn Trading (UK) Limited | Transport & logistics | 2013 |
| Untangled Yarns Ltd | Wholesale & retail | 2023 |
| Vasilisa Ltd | Real estate | 2025 |
| Victoria Boles Limited | Health & social care | 2026 |
| Viewfield Service Centre Limited | Wholesale & retail | 2014 |
| Vlopes Smiles Ltd | Health & social care | 2023 |
| W a Wilson & Co | | 2002 |
| W. Roger Marsh Dentistry Ltd | Health & social care | 2025 |
| Walter B Barrie & Partners | | 1999 |
| Wasifa Rahman Ltd | Hospitality | 2026 |
| WEE Smiles Ltd | Health & social care | 2022 |
| West Dental Holdings Ltd | Financial services | 2026 |
| West Dental Limited | Health & social care | 2016 |
| West Dental Lochside Ltd | Health & social care | 2026 |
| Whyte Orthodontics Ltd | Health & social care | 2025 |
| Willmot, Mcfadzen and Jackson | | 1989 |
| Woll Golf Course Limited | Hospitality | 2001 |
| WORKP13CE Ltd | Professional & scientific | 2023 |
| Yarrow Finance Ltd. | Professional & scientific | 2017 |
| Yarrow Fishery Limited | Agriculture & fishing | 2007 |
| Z.A Clinical Limited | Health & social care | 2026 |
| Z.A.Z Investments Ltd | Real estate | 2022 |
| ZAZ Dental Ltd | Health & social care | 2024 |