| Dunelm Combined Independents Limited | Other services | 2016 |
| Durham Aggregates Limited | Wholesale & retail | 2015 |
| Durham County Engineering Services Limited | Manufacturing | 2011 |
| Durham Plant and Machinery Limited | Manufacturing | 2018 |
| DW Cars Limited | Transport & logistics | 2026 |
| DW Plumbing & Heating (NE) Ltd | Construction | 2024 |
| E a Asset Holdings Limited | Real estate | 2025 |
| E&N Electrical Services Ltd | Construction | 2025 |
| E&R Holdings Ltd | Real estate | 2025 |
| East Coast Fitness PT Ltd | Other services | 2022 |
| East Coast Furniture Limited | Wholesale & retail | 2019 |
| East Coast Furniture Seaham Ltd | Wholesale & retail | 2025 |
| East Durham Artists' Network Ltd | Arts & recreation | 2015 |
| East Durham Community Initiatives Ltd | Hospitality | 1988 |
| East Durham Health & Fitness Community Interest Company | Arts & recreation | 2023 |
| East Shore Mechanical Services Limited | Manufacturing | 2017 |
| Easystoreuk Limited | Wholesale & retail | 2025 |
| Ecatering Ltd. | Extraterritorial | 2022 |
| ECF Seaham Ltd | Arts & recreation | 2021 |
| Edara Ltd | Hospitality | 2025 |
| Edgemark Tax Ltd | Professional & scientific | 2025 |
| Edwards & Bower Limited | Construction | 1990 |
| EL & EL Estates Limited | Real estate | 2025 |
| Eleganz Constructions Limited | Construction | 2016 |
| Elevatecore Services Limited | Information & communication | 2024 |
| Eleven TEN Properties Limited | Real estate | 2025 |
| Elixir Hair Salon Ltd | Other services | 2025 |
| Ellwood Estates Sales & Investments Ltd | Real estate | 2022 |
| Elves Stores Limited | Wholesale & retail | 2021 |
| Empire Arts Ltd | Construction | 2019 |
| Epiphany-Vibe Ltd | Wholesale & retail | 2021 |
| Epoxy Designs Northeast Ltd | Construction | 2025 |
| Eric Southwick & Co Accountants Ltd | Professional & scientific | 2023 |
| ESA Sales Ltd | Other services | 2019 |
| Esschem Europe Limited | Manufacturing | 2000 |
| Eu&Charis Limited | Wholesale & retail | 2024 |
| Everesti Properties Ltd | Real estate | 2025 |
| Evergreen Fencing and Landscaping Ltd | Professional & scientific | 2025 |
| Everneat Cleaning Limited | Administrative & support | 2025 |
| Exclusive Aluminium Ltd | Manufacturing | 2018 |
| Extract Hygiene Services Limited | Administrative & support | 2009 |
| Extract Northeast Ltd | Administrative & support | 2014 |
| FA3TECH Limited | Wholesale & retail | 2024 |
| Falcon Housing Association C.I.C. | Real estate | 2008 |
| Fallowfield Developments Ltd | Construction | 2021 |
| FCT Group Services (UK) Limited | Financial services | 2025 |
| Fenncomm Ltd | Administrative & support | 2016 |
| Ferguson Motor Repairs Limited | Wholesale & retail | 2013 |
| Fighting Fit Physiotherapy Ltd | Health & social care | 2022 |
| Filthy’S Delis Limited | Hospitality | 2026 |
| Firmex Distribution Ltd | Wholesale & retail | 2021 |
| Flamingo Bar & Cafe Limited | Hospitality | 2018 |
| Flexible Energy Consortium (FEC) Limited | Information & communication | 2025 |
| Fonox Ltd | Wholesale & retail | 2025 |
| Food & Fresh Limited | Hospitality | 2026 |
| Footcare UK Limited | Wholesale & retail | 1996 |
| Foster Morton Healthcare Limited | Wholesale & retail | 2024 |
| Fourseen Investments Limited | Professional & scientific | 2023 |
| FR365 Ltd | Public administration | 2022 |
| Fragmented Customs and Restos Ltd | Wholesale & retail | 2023 |
| Fraser and Lewis Investments Limited | Real estate | 2021 |
| Fraser and Lewis Ltd | Other services | 2017 |
| Free the Way | Health & social care | 2004 |
| Fresh Start With Maria Ltd | Wholesale & retail | 2023 |
| Futr Investments Limited | Professional & scientific | 2023 |
| Future Corporate Technologies Limited | Information & communication | 2020 |
| Fyzal Limited | Wholesale & retail | 2013 |
| G and R Brickwork Ltd | Construction | 2015 |
| G B Stewart Limited | Professional & scientific | 2007 |
| G M Packaging (UK) Limited | Wholesale & retail | 2001 |
| G&E Littlewood Limited | Hospitality | 2022 |
| G2G Mobile Solutions Limited | Wholesale & retail | 2023 |
| Gardening Centre Ltd | Agriculture & fishing | 2021 |
| Gary Black Carpet & Flooring Limited | Wholesale & retail | 2022 |
| GAS Boiler Installations North East Limited | Construction | 2020 |
| Gavin Reader Limited | Arts & recreation | 1992 |
| GBK Real Estate Ltd | Real estate | 2024 |
| GC Capital Holdings Limited | Financial services | 2025 |
| GCB Enterprise Ltd | Wholesale & retail | 2026 |
| GD Sweeps Ltd | Administrative & support | 2024 |
| General Paint UK Ltd | Extraterritorial | 2015 |
| George & Sons Murton Limited | Wholesale & retail | 2014 |
| George Fish Bar (Murton) Ltd | Hospitality | 2017 |
| George's (Seaham) Ltd | Hospitality | 2023 |
| Georges Fish Bar Murton Limited | Hospitality | 2025 |
| Gibbons (UK) Holdings Limited | Professional & scientific | 2008 |
| Gibbons (UK) Property Limited | Real estate | 2008 |
| Gibbons International Limited | Wholesale & retail | 1979 |
| Gills FRY FRY Limited | Wholesale & retail | 2007 |
| Gjdigital Ltd | Information & communication | 2024 |
| Glade Howe Holdings Ltd | Real estate | 2015 |
| Glasshouse Publications Ltd | Arts & recreation | 2019 |
| Glebe Centre Enterprises Limited | Hospitality | 2006 |
| Glen Feechan Ltd | Information & communication | 2011 |
| Glkn Ltd | Hospitality | 2018 |
| Gmps Properties Limited | Real estate | 2023 |
| Gmzs Seaham Limited | Wholesale & retail | 2023 |
| Gold Gate Developments Ltd | Real estate | 2026 |
| Gold Star Cleaners Ltd | Administrative & support | 2024 |
| Golden Heart Care Solution Ltd | Health & social care | 2025 |