| 3TO1 Project CIC | Other services | 2019 |
| Ally MAC Joinery Ltd | Construction | 2023 |
| Auto Entry Limited | Construction | 2018 |
| Border Union Railway Company Limited | Transport & logistics | 2011 |
| Brach Construction Limited | Construction | 2011 |
| Cane Hospitality Limited | Hospitality | 2018 |
| Cunningham Haldane & Co Ltd | Professional & scientific | 2003 |
| DCW Property Limited | Real estate | 2024 |
| Garden Rooms Holdings Limited | Other services | 2025 |
| GBX Remediation Ltd | Water & waste | 2024 |
| Glen of Rothes Farm LP | | 2010 |
| Goldfinger Communications Limited | Information & communication | 2012 |
| Graham Mcgrath Limited | Wholesale & retail | 1957 |
| HEA Holdings Ltd | Financial services | 2022 |
| Icom Scottech Limited | Manufacturing | 2000 |
| Jamieson & Gordon Limited | Arts & recreation | 2005 |
| Kinpars Industrial Plastics Limited | Manufacturing | 1963 |
| Kowloon Kitchen Ltd. | Hospitality | 2017 |
| Laser Vision Scotland Ltd | Health & social care | 2013 |
| Lochcarron of Scotland Limited | Manufacturing | 1947 |
| Maiden Mother Crone CIC | Professional & scientific | 2023 |
| Mind Body Spirit Shop Limited | Real estate | 2003 |
| Mosspaul Holdings Ltd | Hospitality | 2019 |
| Murray Blackburn Mackenzie Limited | Information & communication | 2022 |
| Scotdb Limited | Information & communication | 2001 |
| THE Borders Forest Trust | Agriculture & fishing | 1996 |
| UTS 3 Limited | Other services | 2024 |
| Wave Asia Pacific Ltd. | Information & communication | 2019 |
| Whim Peat Limited | Mining & quarrying | 2017 |