| 82 Design Workshop Limited | Professional & scientific | 2021 |
| A Westley Construction Ltd | Construction | 2006 |
| A. P. Papworth Ltd | Construction | 2014 |
| A.K. Marshall GAS Specialists Ltd | Construction | 2002 |
| Alfa Drop Box Ltd | Manufacturing | 2016 |
| Alfa Tail Lifts Limited | Manufacturing | 2001 |
| Alfa Vehicle Conversions Limited | Manufacturing | 2013 |
| Avendis Enterprises Ltd. | Information & communication | 2001 |
| Banks Byrne Limited | Manufacturing | 1996 |
| Bar 147 Limited | Extraterritorial | 2011 |
| Barracuda Systems Limited | Information & communication | 2018 |
| BE Your Best Ltd | Administrative & support | 2004 |
| Beautifully Wooden Ltd | Manufacturing | 2020 |
| BJB Electrical Contracts Ltd | Construction | 2005 |
| Brandi Davey Professional Services Provider Limited | Professional & scientific | 2017 |
| Bunny Racing Ltd | Arts & recreation | 2007 |
| Cairns Didge Property UK Ltd | Real estate | 2004 |
| Cairns Didge UK Limited | Wholesale & retail | 2001 |
| Camba Properties Limited | Real estate | 2018 |
| Cambridge Construction Services Ltd | Construction | 2003 |
| Cambridge Formwork Services Ltd | Construction | 2018 |
| Cambridge Medchem Consulting Limited | Professional & scientific | 2006 |
| Cambridge Mobility Ltd | Wholesale & retail | 2018 |
| Cann I Help (UK) Ltd | Wholesale & retail | 2024 |
| Capital Estate Management Ltd | Real estate | 2002 |
| City Cycle Hire Ltd. | Administrative & support | 2002 |
| CJT SS Ltd | Professional & scientific | 2014 |
| Climate Control (UK) Limited | Administrative & support | 2003 |
| Codon Court Management Limited | Households | 2017 |
| Coel Business Group Ltd | Administrative & support | 2021 |
| Coel Plumbing and Mechanical Services Ltd | Construction | 2017 |
| Compton Hardwick Ltd | Professional & scientific | 2022 |
| Crofter International Limited | Administrative & support | 2020 |
| D-Surf Ltd | Professional & scientific | 2013 |
| Deepcarbon Ltd | Professional & scientific | 2009 |
| Divine Wealth Management Limited | Financial services | 2004 |
| DMC Leisure UK Limited | Extraterritorial | 2003 |
| Domestic Bliss Appointments Limited | Other services | 2009 |
| Drivers Farms Limited | Agriculture & fishing | 1961 |
| Echion Technologies Ltd | Professional & scientific | 2017 |
| Electec Cambridge Ltd | Construction | 2021 |
| Elsenhamhall Limited | Real estate | 2016 |
| Evad Ltd | Information & communication | 1998 |
| Evergreen Web Systems Ltd | Professional & scientific | 2013 |
| Evolvant Technologies Limited | Information & communication | 2021 |
| FIT5 Ltd | Manufacturing | 2011 |
| Fitzwilliam International Consultants Limited | Public administration | 2017 |
| Footprints Cafe Ltd | Other services | 2018 |
| Gleam Cleaning Services (Cambridge) Ltd | Administrative & support | 2014 |
| Gordian Knot Limited | Financial services | 1993 |
| Helical Sustain Limited | Professional & scientific | 2005 |
| Host Bio-Energy UK Limited | Other services | 2014 |
| Invictus Intelligence Ltd | Information & communication | 2024 |
| Ionix Group Limited | Construction | 2024 |
| K.A.L. Fixings UK Limited | Construction | 2004 |
| Magnatech LED (UK) Ltd | Wholesale & retail | 2005 |
| Mantaray Abseil Limited | Construction | 2002 |
| Martin Venn Media Ltd. | Arts & recreation | 2011 |
| Materials Inference Limited | Professional & scientific | 2017 |
| Medcan (UK) Import Ltd | Professional & scientific | 2024 |
| Medcan (UK) Wholesale Ltd | Wholesale & retail | 2024 |
| Mensroom (Cambridge) Ltd | Other services | 2007 |
| OH! Films Limited | Information & communication | 2010 |
| Onecan Ltd | Wholesale & retail | 2023 |
| Paxt Limited | Wholesale & retail | 2000 |
| Philip Boswell Limited | Construction | 1986 |
| Prime Plumbing (Cambridge) Ltd | Construction | 2007 |
| Pulpex Limited | Manufacturing | 2019 |
| Quantrill Computer Limited | Information & communication | 1990 |
| Qui-Gon Limited | Health & social care | 2022 |
| Ramco Group Holdings Ltd | Real estate | 2023 |
| Ramco SEC Limited | Information & communication | 2024 |
| Razamatazzmagic Ltd | Arts & recreation | 2016 |
| Repose Healthcare Ltd | Professional & scientific | 2025 |
| Reuben Read Limited | Administrative & support | 2016 |
| Roza Business Services and Management Limited | Professional & scientific | 2016 |
| Sawston Joinery Limited | Manufacturing | 2001 |
| Sheerline Plastering Limited | Construction | 2018 |
| Slims Barbershop Ltd | Other services | 2025 |
| Snkfg Limited | Professional & scientific | 2018 |
| Spice Star Ltd | Hospitality | 2025 |
| Springwell IT Ltd | Information & communication | 2021 |
| T Mendham Building Ltd | Construction | 2001 |
| Tcjo Investments LLP | | 2025 |
| THE Cambridge Flooring Company Limited | Construction | 2004 |
| THE Young Actors Company Ltd | Arts & recreation | 2003 |
| Tomlinson Steel Limited | Manufacturing | 2003 |
| Total Tools Ltd | Administrative & support | 2013 |
| Tovo Property Ltd | Real estate | 2025 |
| Trugreen Solutions Ltd | Water & waste | 2022 |
| Will Barton Electrical Services Ltd. | Construction | 2010 |
| Xeinadin Cambridge Limited | Professional & scientific | 2002 |
| Zero SUM Ltd | Information & communication | 2005 |