| 123EVPV Ltd | Construction | 2022 |
| 5PICY HUT Ltd | Hospitality | 2026 |
| A & T Macpheat Ltd | Construction | 2025 |
| A. R. L. Software Limited | Information & communication | 2006 |
| ACL Joinery Limited | Construction | 2021 |
| Active Autos UK Ltd | Manufacturing | 2020 |
| Adca (Scotland) Limited | Professional & scientific | 2011 |
| Ahad's Cafe Ltd | Hospitality | 2023 |
| Alpha Car Wash (Dumfries) Limited | Administrative & support | 2025 |
| Alpha PUBS786 Ltd | Hospitality | 2024 |
| Alpha Security (Dumfries) Ltd | Administrative & support | 2022 |
| Alpine Improvements Ltd | Construction | 2026 |
| AM Wind Services Limited | Professional & scientific | 2025 |
| Asandsons Rise Ltd | Hospitality | 2025 |
| Auchengruith Estates Ltd | Hospitality | 2024 |
| Autotec Electrical Ltd | Wholesale & retail | 2023 |
| Beaver Logs Limited | Agriculture & fishing | 2022 |
| Bejms Ltd | Real estate | 2020 |
| Belcher (Holdings) Limited | Extraterritorial | 2008 |
| Belhaven Trout Company Limited | Agriculture & fishing | 2011 |
| Benvenuto Limited | Hospitality | 2018 |
| Browns Manufacturing Limited | Manufacturing | 1987 |
| Cameron Simpson Plastering Limited | Construction | 2018 |
| Carters Cleaning Company Limited | Construction | 2024 |
| CK Property Consultants Ltd | Real estate | 2019 |
| Closeburn Castle Fishings Limited | Agriculture & fishing | 2018 |
| Clydehsi Forensics and Technology Limited | Manufacturing | 2025 |
| Courthill Leisure Ltd | Hospitality | 2017 |
| David Campbell Decorators Limited | Construction | 2021 |
| Drumbuie Renewables Limited | Agriculture & fishing | 2012 |
| Dumfries Estate (Bankend) Limited | Real estate | 2004 |
| Dumfries Skin Clinic Ltd | Other services | 2023 |
| DW & MW Solutions Ltd | Information & communication | 2014 |
| Emma L Gilchrist Limited | Wholesale & retail | 2025 |
| EMS Towage Limited | Professional & scientific | 2024 |
| Espresso Smith Ltd | Wholesale & retail | 2023 |
| Face Aesthetics HSD Limited | Health & social care | 2024 |
| Fenton Barns (Scotland) Limited | Wholesale & retail | 1999 |
| Galloway Giveaways Ltd | Arts & recreation | 2024 |
| GF Facilities Limited | Transport & logistics | 2014 |
| GF Group Limited | Professional & scientific | 2024 |
| GF1 Investments (Holdings) Limited | Professional & scientific | 2018 |
| GF1 Investments Limited | Administrative & support | 2016 |
| GF2 Investments Limited | Professional & scientific | 2019 |
| Glenmuckloch Community Energy Park Limited | Administrative & support | 2014 |
| Glenmuckloch Energy Park | Administrative & support | 2014 |
| Hall's of Scotland Limited | Wholesale & retail | 1981 |
| Happy Hooves Sanquhar C.I.C. | Agriculture & fishing | 2020 |
| Harveys Healthcare Limited | Health & social care | 2008 |
| Institute of Local Television | Information & communication | 1998 |
| J J Campbell Contracts Ltd | Construction | 2015 |
| Jill-Spango Ltd | Real estate | 2020 |
| Kathryn Watt Clinical Psychology Limited | Health & social care | 2024 |
| Kirkconnel Parish Heritage Society | Information & communication | 1999 |
| Kirkhope Farms Ltd | Financial services | 2024 |
| Kmcr Developments Ltd | Real estate | 2020 |
| Kmcr Property Ltd | Construction | 2024 |
| Knockenhair Hospitality Ltd | Hospitality | 2026 |
| LEE Campbell Contracts Ltd | Construction | 2015 |
| Link Mechanical Services Limited | Manufacturing | 2022 |
| LL Plastic Ltd | Wholesale & retail | 2018 |
| Locharbriggs Coldstore Limited | Wholesale & retail | 2005 |
| Logan’S CH Ltd | Hospitality | 2026 |
| Logs 2 U Ltd | Wholesale & retail | 2015 |
| Macpheats of Mennock Ltd | Households | 2024 |
| Memento Mary Limited | Wholesale & retail | 2014 |
| Morag-Spango Ltd | Real estate | 2020 |
| Nith Valley Leaf Trust | Other services | 2009 |
| Nithsdale Incorporated Limited | Hospitality | 2014 |
| Nvlt Trading Ltd | Wholesale & retail | 2023 |
| Premier Convenience Ltd | Wholesale & retail | 2017 |
| Prestige Plumbing and Heating Services Ltd | Construction | 2022 |
| PW Foods Limited | Wholesale & retail | 2006 |
| RAY Black Consulting Limited | Professional & scientific | 2019 |
| RB Electrical and Plumbing Limited | Construction | 2025 |
| RHL Industrial Ltd | Real estate | 2025 |
| Rollem Mechanics Ltd | Wholesale & retail | 2025 |
| Rowancraig Renewables Ltd | Energy | 2024 |
| S & J Weir Ltd | Hospitality | 2019 |
| Sanderson Operations Ltd | Construction | 2025 |
| Sanquhar DG4 Limited | Hospitality | 2018 |
| Stanley Wright Ltd | Real estate | 2011 |
| Sunflower EA Limited | Real estate | 2026 |
| Susan-Spango Ltd | Real estate | 2020 |
| THE Crawick Multiverse Trust | Administrative & support | 2008 |
| THE Hotbox Trailer LLP | | 2026 |
| THE Nethercairn Farm Partnership | | 1998 |
| THE Rubicon Partnership Limited | Administrative & support | 2015 |
| THE Unit Branch Ltd | Hospitality | 2025 |
| Townhead Tyres Limited | Wholesale & retail | 2023 |
| Upper Wellwood Limited | Real estate | 2003 |
| Watchman Renewables Ltd | Energy | 2024 |
| William Duncan Roofing Limited | Construction | 2018 |
| ZG Burgh Properties Ltd | Real estate | 2025 |