| Deepdale Trees Holdings Limited | Agriculture & fishing | 2020 |
| Deepdale Trees Limited | Agriculture & fishing | 1995 |
| Deli Fresh Food Farm Ltd | Wholesale & retail | 2025 |
| Dermatologix Ltd | Manufacturing | 2009 |
| Desire Blue Limited | Wholesale & retail | 2025 |
| Desktop Logic Limited | Extraterritorial | 2011 |
| Dgli Group Ltd | Arts & recreation | 2025 |
| Diagnostic KIT Services Limited | Professional & scientific | 2022 |
| Dirok Ltd | Hospitality | 2020 |
| Dmcqe Ltd | Administrative & support | 2020 |
| Dockside Productions Ltd | Arts & recreation | 2024 |
| Doggy Dreamland Daycare Ltd | Other services | 2025 |
| Doghouse Pictures Limited | Information & communication | 2018 |
| Dokeru Trans Ltd | Transport & logistics | 2014 |
| Dominic English Limited | Information & communication | 2009 |
| Donna Tweedale Styling Limited | Administrative & support | 2017 |
| Dorte Jeffers Ltd | Professional & scientific | 2018 |
| Doug Dawson Creative Limited | Professional & scientific | 2014 |
| Dovecot Property Services Limited | Real estate | 2009 |
| DPM Catering Limited | Hospitality | 2016 |
| Drama Matters Limited | Arts & recreation | 2023 |
| Driveline Systems Limited | Professional & scientific | 1995 |
| DS Building Services (Beds) Limited | Construction | 2022 |
| DT Trading Group Ltd | Wholesale & retail | 2026 |
| Duckie & George Limited | Information & communication | 2016 |
| Duggan Limited | Professional & scientific | 2024 |
| Dulyn Limited | Health & social care | 2023 |
| Duta Services Limited | Transport & logistics | 2020 |
| Dutton Engineering (Woodside) Limited | Manufacturing | 1983 |
| E-Carparts Limited | Wholesale & retail | 2003 |
| EBF IT Ltd | Information & communication | 2020 |
| Echo Aviation Consultancy Ltd | Professional & scientific | 2021 |
| Econergy Limited | Construction | 2017 |
| Econstat Ltd | Professional & scientific | 2011 |
| Ecostar Plan Ltd | Professional & scientific | 2024 |
| ECV Consultancy Limited | Professional & scientific | 1993 |
| Edendreams Ltd | Administrative & support | 2018 |
| Eirma Ltd | Real estate | 2007 |
| EK Food Ltd | Hospitality | 2020 |
| Electrokypol Limited | Manufacturing | 2024 |
| Elemental Stone Limited | Manufacturing | 2025 |
| Elite Motorists Centre (Sandy) Limited | Wholesale & retail | 1996 |
| Elite Roofing (Sandy) Limited | Construction | 2006 |
| Ellektryk Install Limited | Construction | 2026 |
| Elliot Draper Design Ltd | Professional & scientific | 2025 |
| Embedded Software Systems Limited | Information & communication | 1995 |
| EMS R&D Solutions Ltd | Professional & scientific | 2015 |
| Enterprise Contracts Limited | Manufacturing | 2017 |
| Envelope Systems Ltd | Manufacturing | 1944 |
| Erlebach Engineering Ltd | Manufacturing | 2004 |
| Ernestine Edwards Ltd | Information & communication | 2022 |
| Eroja by Abbie Ltd | Wholesale & retail | 2025 |
| Espirehealth Limited | Wholesale & retail | 2009 |
| Esquire & Nine Ltd | Information & communication | 2010 |
| Evans Invest Ltd | Wholesale & retail | 2021 |
| Evateo Limited | Transport & logistics | 2023 |
| Evelin & Brothers Ltd | Wholesale & retail | 2019 |
| Evergreen Local Limited | Wholesale & retail | 2025 |
| Everton Estate Company (NO1) Ltd | Extraterritorial | 2022 |
| Everton Estate Company (NO2) Ltd | Extraterritorial | 2022 |
| Every Body Outdoors CIC | Education | 2023 |
| Everyday IS Mother's Day Ltd | Wholesale & retail | 2025 |
| Express Coffee (Huntingdon) Ltd | Hospitality | 2017 |
| Express Coffee Company Ltd | Hospitality | 2016 |
| Exquisite Escapes Ltd | Wholesale & retail | 2023 |
| Eyeworth Renewable Energy Limited | Energy | 2011 |
| F Mark Limited | Professional & scientific | 2013 |
| Family Care Partnership Limited | Health & social care | 2023 |
| Fandango Meeja Limited | Information & communication | 2014 |
| Fareble Care Limited | Health & social care | 2024 |
| FAT HEN Farm Limited | Agriculture & fishing | 2021 |
| Fettis Films Limited | Information & communication | 1996 |
| Feversham Freerange Eggs Limited | Agriculture & fishing | 2009 |
| Fhgw Limited | Administrative & support | 2013 |
| Fibre Network Limited | Information & communication | 2017 |
| Fiction & Feeling Limited | Arts & recreation | 2016 |
| Fine CUT Media Limited | Information & communication | 2015 |
| Finish X Ltd | Manufacturing | 2018 |
| Fire Retardant Textile Solutions Ltd | Manufacturing | 2017 |
| Fish Soup Productions Limited | Information & communication | 2017 |
| Fitbox Bedfordshire Ltd | Arts & recreation | 2024 |
| FIV5 Tanning and Barbering Ltd | Other services | 2025 |
| Five Counties Legal Services Limited | Professional & scientific | 2014 |
| Flare AIR2AIR Ltd | Energy | 2026 |
| Fleur DE Muse Limited | Wholesale & retail | 2024 |
| Flick Group Ltd | Information & communication | 2025 |
| Flooring Works Ltd. | Construction | 2012 |
| Flxo Ltd | Information & communication | 2022 |
| Flying Restorations Ltd. | Manufacturing | 2012 |
| Flynn's Floors Ltd | Construction | 2025 |
| FOR MEN to Talk Community Interest Company | Administrative & support | 2020 |
| Fordham Consulting Limited | Professional & scientific | 2003 |
| Four Point Five Group Ltd | Information & communication | 2023 |
| Fractals Media Limited | Information & communication | 2021 |
| Franklins Farm Ltd | Wholesale & retail | 2024 |
| Fred Christmas and Company | Agriculture & fishing | 1957 |
| French for Business Ltd | Professional & scientific | 2009 |
| Fresh From Cornwall Limited | Manufacturing | 1996 |
| Fruit Press Limited | Information & communication | 2026 |
| Fund Filer Limited | Information & communication | 2009 |