| Ashwell Property Limited | Construction | 2021 |
| Assd Limited | Wholesale & retail | 2024 |
| ATJ Extraction Ltd | Construction | 2025 |
| Atsi Enterprises Limited | Hospitality | 2021 |
| Audio Creative Design Ltd | Information & communication | 2017 |
| Auto Body Repairs Expert Ltd | Wholesale & retail | 2025 |
| Autocare Solutions Limited | Wholesale & retail | 2024 |
| Avanti Projects Ltd. | Real estate | 2014 |
| AVD Build Ltd | Construction | 2018 |
| Avison Autos Limited | Wholesale & retail | 2012 |
| Ayaz Fish Bar Ltd | Hospitality | 2018 |
| Ayebee Limited | Information & communication | 2012 |
| Ayoba Ltd | Professional & scientific | 2012 |
| B & D Glazing Ltd | Construction | 2025 |
| B Booth Consulting Ltd | Professional & scientific | 2020 |
| B-Noble Healthcare Limited | Wholesale & retail | 2024 |
| B-Therm Ltd | Construction | 2018 |
| B-Therm Renewables Ltd | Construction | 2023 |
| B.Hampton & Sons Vehicle Recovery & Transportation Ltd | Transport & logistics | 2025 |
| B17 Enterprises Ltd | Wholesale & retail | 2019 |
| B4 Holdings Limited | Real estate | 2024 |
| BAD Racing Limited | Arts & recreation | 2021 |
| Balaam Brothers Fencing Ltd | Extraterritorial | 2011 |
| Balaam Brothers Ltd | Construction | 2011 |
| Banks Mills Limited | Real estate | 1955 |
| Banks Truck Centre Limited | Extraterritorial | 1981 |
| Barber IT Project Management Ltd | Information & communication | 2011 |
| Bardeen Ltd | Manufacturing | 2025 |
| Barkway Estates Ltd | Real estate | 2020 |
| Barn OWL Holdings Ltd | Real estate | 2023 |
| Barrack Construction Ltd | Construction | 2022 |
| Barry Peat Plastering & Building Services Limited | Construction | 2014 |
| Bartram (DM) Limited | Construction | 2014 |
| Bartram Land Limited | Construction | 2011 |
| Bartram Lettings Limited | Real estate | 2022 |
| Bartram Manufacturing Limited | Manufacturing | 2007 |
| Bartram Properties Limited | Real estate | 2006 |
| Bartram Timber Frame Limited | Manufacturing | 2014 |
| Bartram Timber Merchants Limited | Professional & scientific | 2012 |
| Bate Farriery Ltd | Agriculture & fishing | 2019 |
| Baystrait Properties Limited | Hospitality | 1966 |
| Beaky’S 'Narrow-Minded' Voyages Ltd | Transport & logistics | 2026 |
| Bean Theory Limited | Manufacturing | 2023 |
| Bear PAW Bakery Ltd | Manufacturing | 2019 |
| Beau Lodge Stud Ltd | Wholesale & retail | 2021 |
| Beauty Spot (Sandy) Limited | Other services | 2017 |
| Bectec Vehicle Repairs Ltd | Wholesale & retail | 2019 |
| Bedford Harriers Athletics Club | Arts & recreation | 2018 |
| Bedford Harriers Events Limited | Arts & recreation | 2018 |
| Bedford Microlight Airsports Limited | Arts & recreation | 2014 |
| Bedfordshire Event Catering Ltd | Hospitality | 2022 |
| BEE Bubble Tea Ltd | Hospitality | 2024 |
| Bell Concrete Ltd | Manufacturing | 2025 |
| Bell Groundworks Ltd | Administrative & support | 2014 |
| Bemermill Limited | Households | 1989 |
| Bene Services Limited | Transport & logistics | 2019 |
| BES Builds Ltd | Construction | 2020 |
| Besafe Limited | Transport & logistics | 2005 |
| Best Dorondel CON Ltd | Construction | 2023 |
| Bgltech Ltd | Construction | 2017 |
| Biggles FM | Information & communication | 2008 |
| Bilzatech Limited | Administrative & support | 1998 |
| BIT Tech IT Solutions Ltd | Information & communication | 2016 |
| BJL Begg Project Consultancy Ltd | Information & communication | 2024 |
| Blackdog Haulage Ltd | Transport & logistics | 2023 |
| Blazing Saddles Coaching Ltd | Education | 2019 |
| Blue Lark Marketing Ltd | Information & communication | 2026 |
| Blue Light Trauma Services Ltd. | Health & social care | 2013 |
| Blue Lightning Solutions Ltd | Professional & scientific | 2019 |
| Blue Skies Ptnrs Holdings Limited | Financial services | 2019 |
| Bluequartz Consulting Ltd | Information & communication | 2025 |
| BNW Injection Moulding Limited | Manufacturing | 2025 |
| Bobi Media Ltd | Information & communication | 2012 |
| BON IT Consultancy Limited | Information & communication | 1999 |
| BON IT Limited | Information & communication | 2011 |
| Bonita Cosmetics Ltd | Other services | 2019 |
| Bophead Limited | Information & communication | 2008 |
| Bowery & Bond Limited | Information & communication | 2012 |
| Boxy TV Ltd | Information & communication | 2015 |
| Boyton Hire Services Limited | Professional & scientific | 1974 |
| BRAIN4AVIATION Consulting Limited | Professional & scientific | 2006 |
| Branchy Limited | Information & communication | 2016 |
| Brandon Parker Ltd | Real estate | 2025 |
| Brasted 1962 Limited | Extraterritorial | 2014 |
| Breathe Digital Support Limited | Manufacturing | 2019 |
| Bregan Mainsflow Limited | Construction | 2017 |
| Bregan Services Limited | Construction | 2016 |
| Brew and Renew Limited | Hospitality | 2025 |
| Brickhill Property Limited | Real estate | 2019 |
| Bricks & Blox Limited | Construction | 2005 |
| Brightstar Hand Car Wash Limited | Wholesale & retail | 2018 |
| Brijpay Ltd | Information & communication | 2020 |
| Brilliant Studios Ltd | Information & communication | 2025 |
| BRJ Holdings Limited | Administrative & support | 1981 |
| Brown FD Solutions Ltd | Professional & scientific | 2017 |
| Bulldog Guitars Limited | Extraterritorial | 2014 |
| Bunceit Ltd | Information & communication | 2026 |
| Burclip Limited | Real estate | 1993 |
| Buzz Associates Limited | Professional & scientific | 1999 |
| Byrne Electrical Ltd | Construction | 2022 |