| Drömhus (Kent) Ltd | Construction | 2021 |
| Dscompliance Limited | Wholesale & retail | 2025 |
| Dstream Holdings Limited | Professional & scientific | 2014 |
| DTD Consult Ltd | Professional & scientific | 2023 |
| Duplatec (UK) Limited | Professional & scientific | 2002 |
| E T Property Developments LLP | | 2020 |
| E.M. Montgomery Limited | Agriculture & fishing | 1988 |
| Early Start Languages Limited | Information & communication | 1999 |
| East Kent Chamber Ltd | Administrative & support | 2012 |
| East Kent Scaffolding Ltd | Construction | 2019 |
| Eastman Solutions Limited | Professional & scientific | 2021 |
| Eastry Pharmacy Limited | Professional & scientific | 2001 |
| Eastwell Ironworks Limited | Manufacturing | 2009 |
| EAT & Sea Limited | Hospitality | 2026 |
| EBR Ltd | Construction | 2022 |
| Ed's Stuff Limited | Wholesale & retail | 2021 |
| Edwards Dining Rooms Limited | Real estate | 1927 |
| Effie Mclean Limited | Wholesale & retail | 2017 |
| Electrical Lighting Solutions Limited | Administrative & support | 2010 |
| Eligochem Limited | Professional & scientific | 2012 |
| Elite Car Sales Ltd | Wholesale & retail | 2014 |
| Elizabeth Langdon Ltd | Wholesale & retail | 2025 |
| Ellis Contract Services Ltd | Agriculture & fishing | 2017 |
| Elysian Trust Financial Ltd | Financial services | 2025 |
| Embassy Global Ltd | Transport & logistics | 2023 |
| Emerge Therapeutic Fostering Ltd | Other services | 2020 |
| Empiridat Ltd | Professional & scientific | 2018 |
| Emstopa Limited | Professional & scientific | 2017 |
| Engage Therapeutic Fostering Ltd | Other services | 2020 |
| EOS Civil Engineering Limited | Construction | 2011 |
| Ephesus (London) Limited | Professional & scientific | 2015 |
| Equestrian HQ Community Interest Company | Education | 2012 |
| Escapement Broadstairs Ltd | Arts & recreation | 2019 |
| Essence Green Ltd | Real estate | 2025 |
| Euro Pacific Corporation Ltd | Construction | 2018 |
| Evenor Consulting Ltd | Professional & scientific | 2013 |
| Event Binoculars Limited | Other services | 2018 |
| Evolution Cornish Properties Limited | Real estate | 2019 |
| Evolution Productions (Plays) Ltd | Arts & recreation | 2013 |
| Evolution Productions Holdings Limited | Financial services | 2020 |
| Evolve Group Training Ltd | Arts & recreation | 2024 |
| Evolve Therapeutic Fostering Ltd | Other services | 2020 |
| Evolve Therapeutic Residential Services Limited | Health & social care | 2023 |
| Evom Academy Limited | Education | 2025 |
| Evom Move Limited | Arts & recreation | 2026 |
| Evom Pilates Limited | Arts & recreation | 2025 |
| Evom Retreats Limited | Arts & recreation | 2025 |
| Exposed Solutions Limited | Information & communication | 2004 |
| Exroid International Limited | Health & social care | 2021 |
| Exroid Ltd | Health & social care | 2016 |
| Exroid Technology Limited | Manufacturing | 2012 |
| Eythorne Building Contractors Limited | Construction | 2005 |
| F & J Events Ltd | Hospitality | 2022 |
| Fathom Solutions Limited | Professional & scientific | 2012 |
| Ferbonik Limited | Wholesale & retail | 2017 |
| FGA Consulting Ltd | Professional & scientific | 2020 |
| Fibonacci Ltd. | Information & communication | 1998 |
| Finally Agency Ltd | Professional & scientific | 2021 |
| First Choice Chinese Ltd | Hospitality | 2025 |
| Fischer Brown Limited | Administrative & support | 2013 |
| Fitinparts Limited | Wholesale & retail | 2020 |
| Fitness Connection (Sandwich) Limited | Arts & recreation | 2003 |
| Five Stars Kent Limited | Hospitality | 2021 |
| Folkestone Food Market Limited | Wholesale & retail | 2020 |
| Forest Homes (Kent) Limited | Construction | 1996 |
| Form Advertising Limited | Other services | 2005 |
| Fort Kent Ltd | Transport & logistics | 2024 |
| Fostival Ltd | Arts & recreation | 2025 |
| Four Candles Films Limited | Information & communication | 2025 |
| Fourth Green Developments Ltd | Construction | 2012 |
| Foxborough Operational Support Ltd | Administrative & support | 2026 |
| Frimley Associates Limited | Professional & scientific | 2026 |
| Frontline Film Advisors Limited | Professional & scientific | 2025 |
| Fundraising Services (UK) Ltd | Wholesale & retail | 2008 |
| G&E Events Ltd | Arts & recreation | 2025 |
| G5D Ltd | Construction | 2020 |
| Garco Pubs and Bars Limited | Hospitality | 2008 |
| Gary Worthington Limited | Construction | 2012 |
| Gatehouse Pharma Services Ltd | Professional & scientific | 2018 |
| Gazen Salts Nature Reserve | Agriculture & fishing | 2006 |
| Gemini Lotto Fundraising Ltd | Professional & scientific | 2014 |
| Genea Biomedx UK Limited | Professional & scientific | 2012 |
| Geode Tribes C.I.C. | Health & social care | 2025 |
| Geoid Ltd | Administrative & support | 2007 |
| GIA Driving Ltd | Education | 2018 |
| Giraffe Enterprises Limited | Wholesale & retail | 2005 |
| GJW Nonclinical Ltd | Professional & scientific | 2026 |
| Glenfield Goring Limited | Administrative & support | 2021 |
| Godmersham Property Services Limited | Manufacturing | 2010 |
| Gordon Engraving (Commercial) Limited | Manufacturing | 1968 |
| Gorilla-Crete Limited | Extraterritorial | 2016 |
| Graecum Services Limited | Professional & scientific | 2020 |
| Granular Therapeutics Limited | Professional & scientific | 2017 |
| Green Bulb Energy Ltd | Energy | 2024 |
| Green Marquis E&R Ltd | Construction | 2022 |
| Green Oak Farm Limited | Agriculture & fishing | 1995 |
| Greyland Universal LLP | | 2010 |
| Ground Transport Services Group Limited | Transport & logistics | 2018 |
| Grow With Physio Ltd | Other services | 2023 |
| Growup Farms Limited | Agriculture & fishing | 2013 |