| Empower Trading Limited | Wholesale & retail | 2023 |
| Empsan Tekstil Limited | Wholesale & retail | 2025 |
| Emqn C.I.C. | Professional & scientific | 2019 |
| Emrj Entertainment Ltd | Information & communication | 2022 |
| Emsi 2000 Ltd | Transport & logistics | 2020 |
| EMU Pictures Limited | Information & communication | 2014 |
| Emune Ltd | Real estate | 2024 |
| EMZ Properties Ltd | Administrative & support | 2024 |
| Enchanted Follicles Ltd | Other services | 2023 |
| Endeavour Quay House Limited | Real estate | 2016 |
| Endeavour Strategic Developments LLP | | 2013 |
| Endeavour Strategic Limited | Hospitality | 2012 |
| Endeavour Worldwide Limited | Transport & logistics | 2010 |
| Endeavourlet Limited | Real estate | 2013 |
| Endopulse UK Limited | Education | 2025 |
| Endurance Risk Consulting Ltd | Professional & scientific | 2024 |
| Enecon Corporation Ltd | Wholesale & retail | 2025 |
| Energey Limited | Information & communication | 2021 |
| Energy Decarbonisation Solutions Ltd | Professional & scientific | 2026 |
| Enermed UK Ltd | Wholesale & retail | 2023 |
| Engine SOS Ltd | Wholesale & retail | 2025 |
| Engraving & Blocking Centre Limited | Manufacturing | 2025 |
| Enhanced Potential Limited | Health & social care | 2003 |
| Enterprise Properties (NO. 1) Limited | Professional & scientific | 2010 |
| Enterprise Properties (NO. 2) Limited | Professional & scientific | 2010 |
| Enterprise RPA Limited | Information & communication | 2018 |
| Enterprise Sales Group Limited | Financial services | 2018 |
| Enterprise Sales Personnel Limited | Administrative & support | 2005 |
| ENTERPRISE360 Services Limited | Information & communication | 2024 |
| Enterprising Minds Limited | Professional & scientific | 2014 |
| Entertainment Television Limited | Information & communication | 2001 |
| Entreeautomotive Ltd | Wholesale & retail | 2022 |
| Entrepreneur Breed Networks Limited | Financial services | 2017 |
| Entroverse Capital UK Ltd | Financial services | 2025 |
| Environmental Nominees Limited | Extraterritorial | 2006 |
| Environmental Services Design Limited | Professional & scientific | 2009 |
| Environmental Surveying & Remediation Ltd | Professional & scientific | 2002 |
| Envision Energy Solutions Ltd | Extraterritorial | 2015 |
| Envision Solutions Ltd | Construction | 2005 |
| Envoy Assist LLP | | 2023 |
| Enzo's Homes (Abbey Woods) Limited | Construction | 2021 |
| EOB Estates Ltd | Real estate | 2023 |
| EOM Holdings Limited | Real estate | 2019 |
| Eovoa Limited | Information & communication | 2026 |
| EPC National Residential Limited | Professional & scientific | 2019 |
| Epic Bargains and Deals Ltd | Information & communication | 2024 |
| Epic Bristol Limited | Information & communication | 2016 |
| Epic Energy Limited | Professional & scientific | 2018 |
| Epipheny Ltd | Administrative & support | 2024 |
| Epoch Company Management Ltd | Administrative & support | 2025 |
| Epsilon 3 Ltd | Extraterritorial | 2011 |
| Equalize Ltd | Wholesale & retail | 2020 |
| Equinox Film/Tv Production Limited | Information & communication | 2003 |
| Equitycircle Ltd | Real estate | 2023 |
| Erez Creative Limited | Professional & scientific | 2025 |
| Ergo Group Ltd | Wholesale & retail | 1996 |
| Ermine Investments UK Ltd | Extraterritorial | 2021 |
| Eruv Consultants UK Ltd | Construction | 2018 |
| ES Assets Limited | Real estate | 2024 |
| ES Video Ltd | Administrative & support | 2025 |
| ES6 Collective Ltd | Wholesale & retail | 2023 |
| Esbee Estates Ltd | Real estate | 2016 |
| Esbee Investments Ltd | Real estate | 2017 |
| Esbridge Ltd | Real estate | 2026 |
| Escapade Cocktail Events Ltd | Hospitality | 2022 |
| Esco Estates Limited | Real estate | 2024 |
| Escrow Hub Ltd | Transport & logistics | 2025 |
| Eseless Limited | Real estate | 2011 |
| Esengo Community CIC | Health & social care | 2025 |
| ESP Consulting and Management Services LLP | | 2011 |
| Esplanade Properties Ltd | Real estate | 2026 |
| Esprit Commerce Limited | Wholesale & retail | 2025 |
| ESS (MCR) Ltd | Hospitality | 2022 |
| Essa Properties Limited | Real estate | 2015 |
| Essa Trading Ltd | Real estate | 2011 |
| Essell Property Group Ltd | Real estate | 2023 |
| Essen Trading Ltd | Wholesale & retail | 2023 |
| Essrim Ltd | Real estate | 2021 |
| EST Transport & Logistic Solutions UK Ltd | Transport & logistics | 2024 |
| Estate Nominees Limited | Professional & scientific | 2005 |
| Estates (M.B.) Limited | Real estate | 2006 |
| Estates United Ltd | Real estate | 2026 |
| Este Maison Ltd | Wholesale & retail | 2024 |
| Este Medical Group (Manchester) Ltd | Health & social care | 2020 |
| Esteebridge Ltd | Extraterritorial | 2003 |
| Estera Estates Ltd | Real estate | 2024 |
| Esterio Ltd | Real estate | 2020 |
| Esther Crown Holdings Ltd | Financial services | 2023 |
| Esther Estates Ltd | Real estate | 2024 |
| Estonefield Ltd | Real estate | 2014 |
| Estry Elkinson Promotions Limited | Professional & scientific | 2002 |
| ET TU Brucey Ltd | Financial services | 2024 |
| Eternal Assets Ltd | Real estate | 2025 |
| Eternal Concierge Ltd | Professional & scientific | 2021 |
| Ethel Crawford Ltd | Wholesale & retail | 2017 |
| Ethovolt Ltd | Information & communication | 2026 |
| Etihad Consulting Ltd | Professional & scientific | 2023 |
| Etna IT Solutions Ltd | Information & communication | 2025 |
| Eton Academy Ltd | Education | 2016 |
| Etsyk Limited | Real estate | 2023 |