| Corner House Retail Limited | Wholesale & retail | 2007 |
| Correti Ltd | Real estate | 2021 |
| Country Buttercups Ltd | Health & social care | 2011 |
| Country Commercial Properties Limited | Construction | 1998 |
| Courts Management Ltd | Construction | 2022 |
| CP Catering Ltd | Hospitality | 2025 |
| Crab & Lobster RYE Ltd | Other services | 2017 |
| Crest Advisory Services Ltd | Professional & scientific | 2023 |
| CRJ Consulting Ltd | Professional & scientific | 2024 |
| Crooked Loft Ltd | Hospitality | 2024 |
| Crowhurst Orthopaedics Limited | Health & social care | 2019 |
| CRW Electrics Ltd | Construction | 2025 |
| Cubik Ltd | Construction | 2022 |
| Custhuri Limited | Wholesale & retail | 2005 |
| CUT the Crap Graphic Design & Photography Ltd | Professional & scientific | 2026 |
| D and P Glass Solutions Ltd | Construction | 2026 |
| D J Pidge Limited | Health & social care | 2012 |
| D.Baker Building and Maintenance Services Ltd | Construction | 2019 |
| D.J.I Consultants Ltd | Financial services | 2012 |
| DA1 Limited | Real estate | 2024 |
| Dado Atelier Ltd | Wholesale & retail | 2021 |
| Danielles Tanning Ltd | Other services | 2026 |
| Davis & Oaks Ltd | Manufacturing | 2023 |
| Dawson & Associates Limited | Professional & scientific | 2014 |
| Dawson Sajik Ltd | Real estate | 2022 |
| DBC RYE Ltd | Hospitality | 2025 |
| Dean & Donaldson Limited | Professional & scientific | 2022 |
| Dearden Ltd | Real estate | 1998 |
| Deenslide Limited | Professional & scientific | 1998 |
| Desert and Dune Consulting Ltd | Professional & scientific | 2025 |
| Design and Build Engineering Limited | Professional & scientific | 2024 |
| Design Studio (Hampshire) Ltd | Professional & scientific | 2010 |
| Detail Developments Sussex Limited | Construction | 2015 |
| DEVIL1 Entertainment Ltd | Administrative & support | 2012 |
| DGM Aromatics Limited | Health & social care | 2016 |
| Dialogue PR Ltd | Professional & scientific | 2010 |
| Directchannel Ltd | Wholesale & retail | 2005 |
| Dixter Taxi Limited | Transport & logistics | 2024 |
| DMI Joinery Ltd | Construction | 2024 |
| Dogan's Fruit & VEG Ltd | Wholesale & retail | 2019 |
| Dolmen Building Conservation & Woodland Ltd | Professional & scientific | 2012 |
| Dowden Capital Management Ltd | Financial services | 2024 |
| DPM Vehicle Maintenance Ltd | Wholesale & retail | 2021 |
| DR Chopra Ltd | Health & social care | 2022 |
| DRL Groundworks Ltd | Construction | 2019 |
| Dropmatics Ltd | Information & communication | 2024 |
| DSC Cricket Limited | Wholesale & retail | 2020 |
| Dstinct Gaming Limited | Arts & recreation | 2020 |
| DTP Consultancy Limited | Professional & scientific | 2017 |
| Dubstar Ltd | Information & communication | 2012 |
| Duet Foundation | Arts & recreation | 2013 |
| Dynamic Conferences & Events Limited | Administrative & support | 2005 |
| E B Sculpture Ltd | Manufacturing | 2009 |
| E.C. Baily & SON (Warlingham) Limited | Real estate | 1951 |
| East Street Ventures Limited | Hospitality | 2007 |
| East Sussex Electrical Ltd | Construction | 2018 |
| East Sussex GAS Limited | Construction | 2016 |
| East Sussex PUB Company Limited | Hospitality | 2010 |
| Eastbourne Wholesale Meat Company Limited | Wholesale & retail | 1988 |
| Easy Living Property Services Limited | Construction | 2003 |
| Ebrofrost UK Limited | Manufacturing | 2015 |
| Electric Palace Studios Limited | Hospitality | 2022 |
| Electrical Utillity Building & Maintenance Services Limited | Construction | 2019 |
| Eleven 11 Holdings Ltd | Wholesale & retail | 2025 |
| Ellen Merchant Ltd | Manufacturing | 2021 |
| Ellis Bros (Funeral Services) Limited | Other services | 2005 |
| Ellis Brothers (Ironmongers) Limited | Wholesale & retail | 1951 |
| Elmley Enterprises Limited | Hospitality | 2001 |
| ELS Installation Solutions Ltd | Construction | 2022 |
| Ementum Ltd | Professional & scientific | 2003 |
| Emma Mccarthy Consultancy Services Ltd | Information & communication | 2013 |
| Empire Worldwide Ltd | Wholesale & retail | 2024 |
| Engineroom (UK) Limited | Information & communication | 2014 |
| English for YOU Limited | Education | 2026 |
| Eurologistix Limited | Administrative & support | 2000 |
| Euronet IT Services Ltd | Information & communication | 2010 |
| Ever-So-Lean Limited | Professional & scientific | 2026 |
| Evernia Ltd | Administrative & support | 2023 |
| EW Production Services Limited | Administrative & support | 2012 |
| EWP Audiovisual Systems Ltd | Information & communication | 2024 |
| EWP Holdings Limited | Financial services | 2024 |
| Exile Communications Ltd | Information & communication | 2009 |
| Experience Sussex Limited | Hospitality | 2025 |
| Extraordinary CIC | Hospitality | 2025 |
| Extraordinary Huts Limited | Hospitality | 2019 |
| Fairlane Limited | Construction | 1973 |
| Fairway Advice Ltd | Professional & scientific | 2012 |
| Faraday House Residents Limited | Real estate | 2004 |
| Farmax Limited | Construction | 1993 |
| Farmhouse Physiotherapy Ltd | Health & social care | 2010 |
| Farrar Vale Limited | Information & communication | 2024 |
| Fbwindpower Ltd | Manufacturing | 2018 |
| FD Recruit (South East) Limited | Administrative & support | 2017 |
| Fenner Holdings Limited | Wholesale & retail | 2002 |
| Fernbank Investments UK Limited | Financial services | 2016 |
| Ferry Road Ltd | Hospitality | 2019 |
| Fewoto Limited | Wholesale & retail | 2025 |
| Figoni ET Falaschi - Paris Limited | Manufacturing | 2025 |
| Financial Service Limited | Financial services | 2002 |
| First Ai.D Automation Ltd | Information & communication | 2026 |