| Dream Nails (Rugeley) Limited | Other services | 2014 |
| DRF Design & Build Ltd | Construction | 2021 |
| DRF Homes Ltd | Extraterritorial | 2021 |
| Drift Holdings Limited | Financial services | 2019 |
| Drinks HUT (UK) Limited | Wholesale & retail | 2026 |
| DRL Holdings Limited | Real estate | 2021 |
| DS Wealth Consultancy Limited | Professional & scientific | 2025 |
| DT Property Improvements Limited | Other services | 2021 |
| DTE Eco Limited | Agriculture & fishing | 2017 |
| Duality Journals Ltd | Extraterritorial | 2025 |
| Duke Furniture Ltd | Professional & scientific | 2012 |
| DUN Agency Ltd | Professional & scientific | 2026 |
| Dynamic Consulting Europe Limited | Professional & scientific | 2003 |
| Dyus Ltd | Wholesale & retail | 2023 |
| E & G Alves Construction Limited | Construction | 2014 |
| E J Services (Midlands) Limited | Construction | 2010 |
| E&O Property Investments Ltd | Financial services | 2023 |
| E-Veac Ltd | Wholesale & retail | 2016 |
| E. B. Construction (Cannock) Limited | Construction | 2016 |
| Eagles Building & Conservation Ltd. | Construction | 2015 |
| Earthcon Civils Ltd | Construction | 2019 |
| East Midland Centre Auto-Cycle Union Limited | Arts & recreation | 1983 |
| Eastfield Limited | Wholesale & retail | 2022 |
| Eastfield Properties (Staffordshire) Limited | Real estate | 2025 |
| Eazie Limited | Professional & scientific | 2009 |
| Ebaby Limited | Wholesale & retail | 2017 |
| Ebim Facilities Limited | Construction | 2022 |
| Echo Sierra Charlie Ltd. | Professional & scientific | 2023 |
| ECI Chelmsford Ltd | Real estate | 2022 |
| Eden BIO Organics R&D Limited | Professional & scientific | 2022 |
| Eden Bio-Organics Limited | Wholesale & retail | 2008 |
| Eden Hair (CG) Limited | Other services | 2025 |
| Edexpro Ltd | Education | 2020 |
| Edible Landscape Ltd | Professional & scientific | 2026 |
| Educational Technology Equipment Limited | Manufacturing | 2004 |
| Educational Technology Equipment Manufacturing Limited | Manufacturing | 2005 |
| Edumis Ltd | Information & communication | 2024 |
| Edwin Ferneyhough Vehicle Sales Limited | Wholesale & retail | 2019 |
| Effelbie Limited | Professional & scientific | 2024 |
| Efftech Limited | Professional & scientific | 2009 |
| Egon Hansen Architects Limited | Professional & scientific | 2023 |
| EI Groundwork Contractors Ltd | Construction | 2014 |
| EI Holdings Ltd | Financial services | 2022 |
| EI Plant Ltd | Construction | 2022 |
| EJC Contracting Limited | Agriculture & fishing | 2022 |
| Elara Pharmaservices Limited | Professional & scientific | 2012 |
| Electrican Ltd | Construction | 2009 |
| Elegant Home Installations Ltd | Construction | 2025 |
| Elevate Care Consultancy Limited | Professional & scientific | 2019 |
| Elevate Group of Companies Ltd | Financial services | 2023 |
| Elevate Hire & Sales Limited | Administrative & support | 2023 |
| Elevate Scaffolding Services Ltd | Construction | 2018 |
| Elevation Business Holdings Limited | Financial services | 2021 |
| Elfint Ltd | Professional & scientific | 2025 |
| Elite Plant Training (Staffordshire) Ltd | Education | 2018 |
| Elizabeth Eloise Hair & Beauty Ltd | Other services | 2018 |
| Elmarini Ltd | Professional & scientific | 2025 |
| Elwell Transport (Repairs) Limited | Wholesale & retail | 1975 |
| Embarba Paragon Lifts Limited | Manufacturing | 1995 |
| Embromatic Print Ltd | Manufacturing | 2018 |
| Emery and Burton Limited | Wholesale & retail | 1978 |
| Empowering U Publications Limited | Information & communication | 2003 |
| Empr Investments Limited | Real estate | 2020 |
| Empyrean CNC Limited | Manufacturing | 2023 |
| Encore (Midlands) Limited | Manufacturing | 1995 |
| Endless Path Limited | Construction | 2024 |
| Enei and Partners Ltd | Transport & logistics | 2024 |
| Energy and Environment Strategies and Solutions Ltd | Professional & scientific | 2021 |
| Enlighten Connections Ltd | Education | 2021 |
| Envitec Biogas Service UK Limited | Manufacturing | 2010 |
| Envitec Biogas UK Limited | Construction | 2007 |
| Epic (Integrity Assured) Ltd | Other services | 2011 |
| Epic Frames Ltd | Other services | 2015 |
| EPS Property Limited | Real estate | 2019 |
| EPS2 Limited | Real estate | 2024 |
| Equinimity Wellbeing CIC | Other services | 2023 |
| ES26 Ltd | Hospitality | 2018 |
| Esprit Spa Recruitment Ltd | Administrative & support | 2016 |
| Estate Signs & Print Limited | Manufacturing | 1996 |
| ET Biogas Ventures Limited | Energy | 2013 |
| ETA Process Plant Limited | Professional & scientific | 2018 |
| Ethical ME Limited | Wholesale & retail | 2019 |
| Eunl Limited | Transport & logistics | 2024 |
| Euphoria Hair & Beauty Ltd | Other services | 2024 |
| Eurotec Distribution Limited | Wholesale & retail | 1982 |
| Eurovillas (Spain) Limited | Professional & scientific | 2001 |
| EVC Music Publications Ltd | Information & communication | 2016 |
| Event Bar Hire Ltd | Administrative & support | 2021 |
| Event Furniture Limited | Manufacturing | 2005 |
| Event Hire London Limited | Administrative & support | 2011 |
| Event Refurb and Hire Ltd | Administrative & support | 2011 |
| Event Security Ltd | Administrative & support | 2026 |
| Eventex Hire Ltd | Manufacturing | 2010 |
| Evergreen Blooms Limited | Wholesale & retail | 2024 |
| Evergreen Intermediate Limited | Wholesale & retail | 2020 |
| Everlec Limited | Wholesale & retail | 2025 |
| Evolve Planning and Design Ltd | Professional & scientific | 2014 |
| Evonco Ltd | Real estate | 2019 |
| Excellerator Limited | Professional & scientific | 2019 |
| Excloosive Limited | Water & waste | 2001 |