| True Life Productions CIC | Arts & recreation | 2024 |
| Trulight Limited | Extraterritorial | 2000 |
| Trusted Tax Partners Limited | Professional & scientific | 2026 |
| Tsce Consulting Limited | Professional & scientific | 2013 |
| TTP Venture Fund L.P. | | 1998 |
| Tudor Court (Kneesworth) Management Company Limited | Professional & scientific | 1990 |
| Tunbridge Consulting Limited | Professional & scientific | 2011 |
| Tunsglade Limited | Real estate | 1997 |
| Tunstal Property Developments Limited | Real estate | 1962 |
| Turvey Development Consultants Limited | Construction | 2003 |
| Tutis Construction Ltd | Real estate | 2018 |
| Tutis Properties Limited | Real estate | 2012 |
| TWG Hold Ltd | Real estate | 2025 |
| Twin Parks Limited | Real estate | 2013 |
| Twin Roofing Ltd | Construction | 2022 |
| Two Giant Leaps Limited | Professional & scientific | 2010 |
| Two Nineties Ltd | Wholesale & retail | 2023 |
| Twyford Seeds Limited | Extraterritorial | 1936 |
| Txgt Ltd | Wholesale & retail | 2021 |
| Tyrolese (40) Limited | Real estate | 1985 |
| Tyrrells Equine Clinic Limited | Professional & scientific | 2007 |
| U H Films Limited | Information & communication | 2010 |
| Ubangi Timber Limited | Wholesale & retail | 1994 |
| Ubuy Computers Limited | Information & communication | 2021 |
| UK CX International Holding Group PLC | Administrative & support | 2020 |
| UK Glaze Ltd | Construction | 2025 |
| Ultra Soil Solutions Ltd | Administrative & support | 2010 |
| Unbuttoned Limited | Hospitality | 2016 |
| Unchained Labs Royston Ltd. | Professional & scientific | 2001 |
| Unchained Labs UK Malvern Ltd | Administrative & support | 2018 |
| Uncover Design Limited | Professional & scientific | 2008 |
| Unfathomable Audio Limited | Information & communication | 2022 |
| Unicorn Silver Limited | Wholesale & retail | 2007 |
| Unique Vehicle Detailing Ltd | Wholesale & retail | 2023 |
| United E Property Limited | Real estate | 2022 |
| Universal Hydrogen LP | | 2011 |
| University Games UK Limited | Administrative & support | 1993 |
| Upcher Holdings 2024 Ltd | Real estate | 2023 |
| Uphall Cambridge Limited | Financial services | 2014 |
| Uprt Ltd | Transport & logistics | 2020 |
| Urban Bar Limited | Wholesale & retail | 2003 |
| Ursae Limited | Arts & recreation | 2013 |
| USA Chicken and Pizza Royston Ltd | Hospitality | 2023 |
| USA Royston Ltd | Hospitality | 2026 |
| Ux&Ui LAB Ltd | Information & communication | 2019 |
| V P C Limited | Real estate | 2011 |
| Valtice Consultants Limited | Transport & logistics | 2016 |
| Vanguard Fire & Life Safety Solutions Ltd | Construction | 2026 |
| Vantage Risk Group Limited | Professional & scientific | 2026 |
| Varsity Building Consultancy Ltd | Professional & scientific | 2023 |
| Varsity Consulting Ltd | Professional & scientific | 2017 |
| VB Trophies Limited | Other services | 2025 |
| VDR Build Ltd | Construction | 2018 |
| Vegan ALL Sorts LLP | | 2019 |
| Venom Limited | Wholesale & retail | 1999 |
| Verglasen Consulting Limited | Professional & scientific | 2024 |
| Verne House Management Limited | Households | 1979 |
| Vernon Hales Consultancy Limited | Education | 2021 |
| Vertical Recordings Limited | Information & communication | 2001 |
| Veryopinionatedpeople Ltd | Information & communication | 2023 |
| Veryworth Limited | Professional & scientific | 1985 |
| VHL Aerospace Ltd | Manufacturing | 2025 |
| Victor Halls Farms Limited | Agriculture & fishing | 1958 |
| Victoria Beechey Consultancy Limited | Professional & scientific | 2014 |
| Victoria Crescent 1972 Limited | Extraterritorial | 2014 |
| Victoria Green Lane Management Company Limited | Households | 2025 |
| Victory Court Management Limited | Households | 1992 |
| Village Care and Assitance Ltd | Health & social care | 2025 |
| Vine Educational Consulting Ltd | Education | 2025 |
| Vinery Road Films Limited | Information & communication | 2019 |
| VIP Day Centre Royston Limited | Health & social care | 2024 |
| Vision Leaders Limited | Professional & scientific | 2024 |
| Vita in Motu Limited | Real estate | 2025 |
| Vitra Pharmaceuticals Ltd | Manufacturing | 1993 |
| Vizula Limited | Information & communication | 2019 |
| Voidtrack Ltd | Professional & scientific | 2023 |
| Volac International Limited | Manufacturing | 1991 |
| Volac Wilmar Feed Ingredients Holdings Limited | Manufacturing | 2015 |
| Volac Wilmar Feed Ingredients Limited | Manufacturing | 2015 |
| Vortex Biotech Holdings Ltd | Professional & scientific | 2022 |
| Vortex Liquid Biopsy Solutions Limited | Professional & scientific | 2020 |
| Vr-Tek Ltd | Information & communication | 2007 |
| Vranity Ltd | Information & communication | 2023 |
| Vsai Limited | Information & communication | 2021 |
| W & R Parrish (Farms) Limited | Agriculture & fishing | 1976 |
| W J Mead (Builder) Limited | Construction | 2006 |
| W J Mead Lettings Ltd | Real estate | 2017 |
| W M Roberts Limited | Construction | 2009 |
| W Q Limited Partnership | | 2008 |
| W R Pape Limited | Manufacturing | 2009 |
| W.B.Hutley(Farms)Limited | Extraterritorial | 1958 |
| W.C.A. Limited | Professional & scientific | 2014 |
| W.T.Doggett & Co Limited | Agriculture & fishing | 1957 |
| W8 Freehold Limited | Real estate | 2011 |
| Wallis Building 22 Limited | Administrative & support | 2004 |
| Walters and Walters Limited | Manufacturing | 1955 |
| Wanda Connected Health Systems Limited | Health & social care | 2020 |
| Warmans 1859 Limited | Real estate | 2021 |
| Warmans Property Management Limited | Households | 2009 |
| Warmhomes Limited | Real estate | 1999 |