| Alba Hospitality Limited | Hospitality | 1991 |
| Anderson Farmers Limited | Agriculture & fishing | 2002 |
| Arkle Recruitment Limited | Administrative & support | 2007 |
| BCM Steeplejacks Limited | Construction | 2007 |
| Border Concrete Products Limited | Manufacturing | 1952 |
| Border Roofing Ltd. | Construction | 2001 |
| Border Union Limited | Real estate | 2001 |
| Borders Quality Beef & Lamb Ltd | Agriculture & fishing | 2004 |
| Borders Quality Beef Ltd | Agriculture & fishing | 2004 |
| Bryce of Kelso Limited | Construction | 2011 |
| Cheers Group Limited | Hospitality | 2006 |
| Clark Farming Ltd | Administrative & support | 2011 |
| Clearvue Accountancy Ltd | Professional & scientific | 2025 |
| Cotfield Farmers | | 1997 |
| D. & D. W. D. Thomson | Agriculture & fishing | 1954 |
| D. Robertson & Sons (POT 80) Ltd | Agriculture & fishing | 2010 |
| Dobbie & Michie Limited | Construction | 2003 |
| Dryburgh Mains Farmers | | 1990 |
| Emtelle EOS Holdings Limited | Manufacturing | 2021 |
| Emtelle UK Limited | Manufacturing | 1982 |
| Eric Gillie Limited | Transport & logistics | 1974 |
| Farm of Fairnington | | 1986 |
| Ferguson & Aitkin Limited | Construction | 2002 |
| Fleming Farms Limited | Agriculture & fishing | 2012 |
| Floors Energy Limited | Manufacturing | 2008 |
| Fraser & Renwick Limited | Construction | 2003 |
| Frogden Farm and Company | | 1990 |
| Frontdesk.Co.Uk Limited | Administrative & support | 2003 |
| G M & C J Usher | | 1987 |
| G. Mcgimpsey & Co. (Demainholm) | | 1994 |
| Glebehead Farming Company | | 1987 |
| Gledswood and Redpath Farm | | 1992 |
| Goffs UK Ltd | Other services | 1976 |
| Goodson Farming Ltd | Agriculture & fishing | 2011 |
| Gray and Company Contracts Limited | Administrative & support | 2002 |
| GVG Project Engineering Limited | Professional & scientific | 1997 |
| Howahill Farming Company Limited | Agriculture & fishing | 2003 |
| Inside Media Group (Scotland) Limited | Transport & logistics | 2023 |
| J & S Anderson (West Boonraw) & Company | | 1988 |
| J. Mcallan & Sons Limited | Construction | 2003 |
| J.Hepburn & Company (Northhouse) | | 1987 |
| James Swinton and Company Limited | Construction | 1975 |
| JED Bar Supplies Limited | Wholesale & retail | 1984 |
| John Nairn Joiners Limited | Construction | 2006 |
| JRW Investments Limited | Real estate | 2007 |
| Kelso Races Limited | Other services | 1928 |
| Kenneth Forbes (Holdings) Limited | Professional & scientific | 1983 |
| Landmaps Limited | Administrative & support | 1997 |
| Linden LEA Landscaping & Tree Services Limited | Agriculture & fishing | 2003 |
| Livestock Marketing (Borders) Limited | Agriculture & fishing | 1995 |
| Lloyds Tractors Limited | Extraterritorial | 1981 |
| Lochtower Limited | Agriculture & fishing | 1997 |
| Lothian Estates | Real estate | 1988 |
| M. & J. Ballantyne Limited | Construction | 1964 |
| Martinshouse Design and Marketing Limited | Professional & scientific | 1981 |
| Melrose RFC Limited | Extraterritorial | 2006 |
| Messieurs Macdonald Partners (Holydean) | | 1997 |
| MHS Studio Limited | Households | 2022 |
| Milestone Garden & Leisure Limited | Wholesale & retail | 2005 |
| Millar Farming Holdings Limited | Financial services | 2023 |
| Millar Farming Limited | Agriculture & fishing | 2023 |
| P Collins (Fenwick) & Co. | | 1997 |
| P.J. and L. Dixon | | 1994 |
| Plexus Corp (UK) Limited | Manufacturing | 1993 |
| Plexus Corp. Limited | Professional & scientific | 2000 |
| Plexus Technology Group Limited | Extraterritorial | 1995 |
| Queens Head Hotel Limited (the) | Hospitality | 1986 |
| R & a Wilson Limited | Agriculture & fishing | 2002 |
| Redpath Farms | | 1994 |
| Sandbed Launderette & DRY Cleaners Limited | Other services | 2002 |
| Sandystones Ltd | Agriculture & fishing | 2004 |
| Scotcra Ltd. | Professional & scientific | 2006 |
| Scotson Farms Limited | Agriculture & fishing | 1970 |
| South Uist Estates Charitable Trust | Administrative & support | 2001 |
| ST. Mary's School, Melrose | Education | 1945 |
| Sunlaws Development Company Limited | Hospitality | 1989 |
| Teviotdale Plumbing & Heating Limited | Construction | 2016 |
| THE A.J.Birnie Partnership | | 1990 |
| THE Abbotsford Trading Company Limited | Wholesale & retail | 2007 |
| THE Abbotsford Trust | Administrative & support | 2007 |
| THE Armitage Fendyhall NO. 1 Partnership | | 1996 |
| THE Armitage Fendyhall NO. 2 Partnership | | 1996 |
| THE Dodds Hunthill Partnership | | 1996 |
| THE Firm of Glebehead Farming Company | | 1996 |
| THE Jackson Scraesburgh Partnership | | 1996 |
| THE Linthill Partnership | | 1993 |
| THE Linton Bankhead Farming Partnership | | 2001 |
| Think Fitness Limited | Arts & recreation | 2001 |
| WGW Property Limited | Real estate | 2007 |
| Whitchester Christian Centre | Hospitality | 1985 |
| ZA Management Holdings Ltd | Real estate | 2019 |