| Adventure Lane CIC | Other services | 2024 |
| Argyle PPE Consultants Ltd | Professional & scientific | 2023 |
| Bute Community Land Company | Other services | 2009 |
| Bute Diving Ltd | Agriculture & fishing | 2024 |
| Bute Golf Ltd | Arts & recreation | 2025 |
| DGX Ltd | Professional & scientific | 2022 |
| E Corbett Farming Ltd | Agriculture & fishing | 2023 |
| Excel Clyde Ltd. | Construction | 1985 |
| Gamba's Letting Limited | Real estate | 2014 |
| George Hanson (Trading) Limited | Construction | 2021 |
| H Greene Limited | Administrative & support | 2001 |
| Halcyon Habitats Ltd | Agriculture & fishing | 2025 |
| Happy Tartan Ltd | Wholesale & retail | 2024 |
| Host 55 Ltd | Manufacturing | 2023 |
| House & Linen Company Ltd | Professional & scientific | 2021 |
| Howat's Housekeeping & Property Services Ltd | Administrative & support | 2023 |
| Howat's Laundry Services Ltd | Other services | 2023 |
| Isle of Bute BID Limited | Other services | 2020 |
| Isle of Bute Construction Limited | Construction | 2021 |
| Isle of Bute Jewellery Limited | Wholesale & retail | 1993 |
| John Mackirdy Ltd | Transport & logistics | 2012 |
| KEN Duncan Ltd | Professional & scientific | 2009 |
| Kilchattan Bay Kayaking Ltd | Arts & recreation | 2021 |
| Kinship & Wellbeing C.I.C. | Education | 2026 |
| Lonach Argyle Ltd | Real estate | 2025 |
| Marquees of Bute Ltd | Other services | 2021 |
| Mcmillan Heating & Renewables Limited | Construction | 2025 |
| Mobara Retails Ltd | Wholesale & retail | 2018 |
| Orissor Trust Ltd. | Agriculture & fishing | 2008 |
| Phytogenomics LLP | | 2022 |
| Robb & Associates Ltd | Financial services | 2014 |
| Scalpsie Kennels Ltd | Wholesale & retail | 2024 |
| Sotoberry Ltd | Wholesale & retail | 2025 |
| Tattie Bagel Ltd | Hospitality | 2026 |
| Taverna Bar (Rothesay) Ltd | Hospitality | 2025 |
| Terry's Auto Repairs Ltd. | Manufacturing | 2007 |
| THE Boatyard Limited | Transport & logistics | 1997 |
| THE Sula Collective Ltd | Wholesale & retail | 2026 |
| THE Waiting Room Rothesay Ltd | Hospitality | 2026 |