| Rotherham Enterprise Agency Limited | Real estate | 1982 |
| Rotherham Ethnic Minority Alliance Limited | Other services | 2003 |
| Rotherham Federation of Communities Ltd | Other services | 2006 |
| Rotherham Fireplace Centre Limited | Wholesale & retail | 2005 |
| Rotherham Football Centre Ltd | Arts & recreation | 2015 |
| Rotherham Glass & Glazing Limited | Construction | 1998 |
| Rotherham Golf Club Limited | Arts & recreation | 1929 |
| Rotherham Joinery Limited | Manufacturing | 2002 |
| Rotherham Marble & Granite Ltd | Manufacturing | 2025 |
| Rotherham Memorial Company Limited | Professional & scientific | 2018 |
| Rotherham Metro Limited | Arts & recreation | 2006 |
| Rotherham Mini Digger Hire Ltd | Extraterritorial | 2016 |
| Rotherham Open Arts Renaissance C.I.C. | Arts & recreation | 2006 |
| Rotherham Parents Forum Limited | Other services | 2011 |
| Rotherham Parties Ltd | Professional & scientific | 2024 |
| Rotherham Pet Supplies Ltd | Wholesale & retail | 2024 |
| Rotherham Podiatry Clinic Ltd | Health & social care | 2009 |
| Rotherham Property Company Limited | Real estate | 1964 |
| Rotherham Property Management Limited | Real estate | 2023 |
| Rotherham Reboring Service Limited | Wholesale & retail | 1977 |
| Rotherham Recovery Community Lero CIC | Health & social care | 2026 |
| Rotherham Refinishing Supplies Limited | Wholesale & retail | 2009 |
| Rotherham Respiratory Limited | Education | 2008 |
| Rotherham Rise (Trading) Limited | Health & social care | 2019 |
| Rotherham Roofing & Pointing Ltd | Construction | 2025 |
| Rotherham Rugby Club Limited | Arts & recreation | 2007 |
| Rotherham Titans Rugby Ltd | Arts & recreation | 2020 |
| Rotherham Tower Developments Ltd | Real estate | 2022 |
| Rotherham Town Football Club Ltd | Arts & recreation | 2024 |
| Rotherham Tyre Centre Ltd | Wholesale & retail | 2025 |
| Rotherham United Community Trust | Arts & recreation | 2007 |
| Rotherham United Football Club (Rufc) Limited | Arts & recreation | 2008 |
| Rotherham Vapes Ltd | Wholesale & retail | 2019 |
| Rotherham West Community Connections CIC | Professional & scientific | 2023 |
| Rotherplumb Ltd | Construction | 2025 |
| Rotherstoke Flats Co. Limited | Households | 1981 |
| Rotherwood Installations Limited | Construction | 2011 |
| Roulette London Ltd | Wholesale & retail | 2016 |
| Rouse Haulage Ltd | Transport & logistics | 2018 |
| Route One Training and Development Limited | Professional & scientific | 2002 |
| Rowan Property Rentals Ltd | Real estate | 2024 |
| Rowley Surveying Ltd | Construction | 2024 |
| Rowson Limited | Health & social care | 2002 |
| Roxcan Limited | Education | 2024 |
| ROY Bailey Transport Limited | Transport & logistics | 2002 |
| ROY Hatfield (Holdings) Limited | Financial services | 2016 |
| ROY Hatfield Group Ltd | Extraterritorial | 2018 |
| ROY Hatfield Limited | Water & waste | 1978 |
| Royal Oak Yorkshire Ltd | Hospitality | 2025 |
| Royall Rendering Ltd | Construction | 2023 |
| Rozh Barber Shop Ltd | Other services | 2023 |
| Rozy Limited | Wholesale & retail | 2025 |
| RP Charnock Accountants Ltd | Administrative & support | 2024 |
| RP Charnock Ltd | Professional & scientific | 2015 |
| RP Companies Limited | Professional & scientific | 2024 |
| RP Resourcing & Planning Limited | Information & communication | 2026 |
| RPC Alloys Limited | Professional & scientific | 2010 |
| RPL Drylining Ltd | Construction | 2019 |
| RPR Rail Ltd | Administrative & support | 2015 |
| RPV Contractors Limited | Professional & scientific | 2023 |
| RRF Joinery Services Limited | Construction | 2018 |
| Rrfkb Limited | Construction | 2023 |
| RRW Contracting Services Limited | Construction | 2025 |
| RS Platinum Gym Ltd | Professional & scientific | 2024 |
| RS Property and Lettings Limited | Real estate | 2025 |
| RS Quality Auto Body Repairs Ltd | Wholesale & retail | 2025 |
| RS Tradewave UK Ltd | Wholesale & retail | 2025 |
| RS246 Limited | Professional & scientific | 2012 |
| RSB Barber Ltd | Other services | 2025 |
| RSB Communications Ltd | Information & communication | 2018 |
| RSD Consultancy Ltd | Professional & scientific | 2017 |
| RSK Electrical Limited | Construction | 2019 |
| Rsng Properties Ltd | Real estate | 2021 |
| RSP Constructions Services Limited | Construction | 2017 |
| RSY (Air Conditioning) Limited | Construction | 1984 |
| RT Companies Ltd | Wholesale & retail | 2025 |
| RT Renovations & Maintenance Yorkshire Ltd | Professional & scientific | 2025 |
| RTD Mechanical Limited | Manufacturing | 2023 |
| Rtmc Rigging Limited | Information & communication | 2025 |
| RTS Contracting Limited | Construction | 2020 |
| Rtsl Ltd | Wholesale & retail | 2025 |
| Rubies Limited | Health & social care | 2014 |
| Rudd Property Group Limited | Real estate | 2026 |
| Rudge Properties Limited | Construction | 2003 |
| Rugby Gameday Ltd | Arts & recreation | 2020 |
| RUH Property Holdings Ltd | Real estate | 2026 |
| Rukasa Properties Ltd | Real estate | 2026 |
| Rumenovi Ltd | Hospitality | 2023 |
| Rumq Limited | Information & communication | 2009 |
| RUN4LONG Ltd | Wholesale & retail | 2025 |
| Runcomm Events CIC | Arts & recreation | 2015 |
| Rupert Housing and Support Ltd | Real estate | 2025 |
| Rush House Ltd | Hospitality | 1995 |
| Rushforth Construction Ltd | Construction | 2023 |
| Russ Consulting Limited | Professional & scientific | 2014 |
| Russam Property Holdings Limited | Real estate | 2024 |
| Russell House (South Anston) Management Company Limited | Real estate | 2008 |
| Russell Mechanical Engineering Limited | Professional & scientific | 2022 |
| Ruth's Rules Ltd | Health & social care | 2021 |
| RV Transport Ltd | Transport & logistics | 2013 |