| Rvel Edinburgh Veins Limited | Health & social care | 2024 |
| S. H. Tech Solutions Ltd | Information & communication | 2026 |
| Singularity Technology Ltd | Information & communication | 2026 |
| Sisaltech Ltd. | Manufacturing | 2012 |
| SJH Orthopaedics Limited | Health & social care | 2023 |
| SOS: School of Self Ltd | Health & social care | 2023 |
| SOX Property Holdings Ltd | Real estate | 2022 |
| Springfield Energy Limited | Energy | 2015 |
| Stark Property Holdings Limited | Real estate | 2021 |
| Strathallan Hydrogen Limited | Energy | 2023 |
| Sunnybrae Property Ltd | Construction | 2018 |
| SWITCH2DIGITAL Ltd | Professional & scientific | 2025 |
| TBM Property Renovation Limited | Construction | 2015 |
| THE Conifer Breeding Co-Operative Ltd | Agriculture & fishing | 2014 |
| THE House of Support and Listening CIC | Education | 2025 |
| THE Orrock Edit Ltd | Professional & scientific | 2025 |
| THE Reading Compass Ltd | Other services | 2025 |
| THE Skin & Lifestyle Edit. Ltd | Other services | 2023 |
| THE Woof Pack (Midlothian) Ltd | Other services | 2022 |
| Thillai Pandias (Tartan Kissed South Indian Classics) Ltd | Hospitality | 2025 |
| Thillaipandias Ltd | Hospitality | 2025 |
| Tiree Design 59 Ltd | Construction | 2009 |
| TR Emerging Solutions Limited | Professional & scientific | 2024 |
| Tree House Publishing LLP | | 2025 |
| Tsporch Property Ltd | Real estate | 2021 |
| Ujamaa Spice Ltd | Wholesale & retail | 2022 |
| Uniquepelek Ltd | Health & social care | 2016 |
| V.W.A Properties Limited | Real estate | 2025 |
| Valyou Living Limited | Health & social care | 2025 |
| Welcome Club Ltd | Administrative & support | 2025 |
| Wildlife Surgery International Ltd | Professional & scientific | 2017 |
| Woods Taxi Partnership | | 1999 |
| Wright Automotive Ltd | Wholesale & retail | 2012 |
| Wylie Coyote Couriers Ltd | Transport & logistics | 2020 |
| ZAE Healthcare Ltd | Health & social care | 2023 |
| Zephyrus Green Energy Ltd | Energy | 2021 |