| UK Hand and Wrist Ltd | Health & social care | 2023 |
| UK Sales Agent Ltd | Information & communication | 2025 |
| UK Tax Advisers Limited | Professional & scientific | 1998 |
| Unfailing Grace Ltd | Information & communication | 2013 |
| Unicom (2015) Limited | Wholesale & retail | 2015 |
| Unicorn Holiday Lets Ltd | Hospitality | 2024 |
| Uniq Technology Ltd | Information & communication | 2004 |
| Unique Nails (Romsey) Limited | Other services | 2013 |
| Universal Marine Medical Limited | Wholesale & retail | 2008 |
| University of Winchester Academy Trust | Education | 2015 |
| Unlimited Projects Ltd | Administrative & support | 2021 |
| Upper Ashfield Management Company Limited | Real estate | 2012 |
| Upper Glass Limited | Professional & scientific | 2012 |
| Urbanityx Ltd | Construction | 2014 |
| UTILITIES4LESS (Southampton) Limited | Energy | 2016 |
| Utmost Photography Ltd | Professional & scientific | 2021 |
| V&A Healthcare Capital Ltd | Extraterritorial | 2025 |
| V&A Integrated Medical Care Ltd | Health & social care | 2025 |
| V&R Fabrications Limited | Manufacturing | 2014 |
| V8 Finance Consulting Ltd | Real estate | 2012 |
| Valour Construction Ltd | Construction | 2023 |
| Vapelicious Ltd | Wholesale & retail | 2019 |
| VDK Medical Services Limited | Health & social care | 2019 |
| Vehicle Solutions (Southern) Limited | Wholesale & retail | 2009 |
| Veltrucks Limited | Manufacturing | 2003 |
| Verity Medical Limited | Manufacturing | 1998 |
| Vermin Control Ltd | Other services | 2019 |
| Vermin Ltd | Other services | 2019 |
| Vertus HR Limited | Administrative & support | 2016 |
| Verve Accounting Group Limited | Professional & scientific | 2018 |
| VFH Choir | Professional & scientific | 2014 |
| Vibrant Housing Services CIC | Health & social care | 2024 |
| Vicsol Foods Limited | Hospitality | 2022 |
| Victoria Rose Dramatherapy Limited | Extraterritorial | 2019 |
| Victoria School of English Limited | Education | 1976 |
| Victory Holdings Limited | Professional & scientific | 1988 |
| Victory Marine Engineering Ltd | Manufacturing | 2012 |
| Victory Marine Services Limited | Manufacturing | 2000 |
| Victory Storage Ltd | Administrative & support | 2017 |
| Victory Yard Limited | Real estate | 1987 |
| Vida Amorosa Ltd | Real estate | 2007 |
| Vida Physio Limited | Health & social care | 2024 |
| Vidhi & Alok Sharma Associates Limited | Health & social care | 2013 |
| Vigil AI Ltd | Information & communication | 2017 |
| Villa Club Limited | Administrative & support | 2006 |
| Village VEG Box Co Limited | Wholesale & retail | 2019 |
| Villiers Property Services Limited | Professional & scientific | 2013 |
| Vipservices.Info Ltd | Information & communication | 2011 |
| Viridifolium Limited | Construction | 2025 |
| Virtual Vibes Limited | Professional & scientific | 2021 |
| Vision Architects Limited | Professional & scientific | 2010 |
| Viswanathan Consulting Ltd | Health & social care | 2016 |
| Vitabode Limited | Wholesale & retail | 2025 |
| Vital Chiropractic Clinic Limited | Health & social care | 2022 |
| Vitria Technology Limited | Information & communication | 1999 |
| VNK Engineering Ltd | Professional & scientific | 2018 |
| Volkstech Romsey Ltd | Wholesale & retail | 2024 |
| Volume Hair Design (at 59) Limited | Other services | 2004 |
| VON ARX Property Holdings Ltd | Real estate | 2023 |
| VON ARX Spine & Physio Limited | Health & social care | 2009 |
| Vrail Ltd | Information & communication | 2024 |
| Wags With Willow Ltd | Other services | 2023 |
| Wales & West Decorators Limited | Construction | 2006 |
| Walks & Whimsy Ltd | Hospitality | 2025 |
| Wallace & Frost Estates Ltd | Real estate | 2022 |
| Wansabai Thai Massage Ltd | Other services | 2021 |
| Wardan Limited | Health & social care | 2022 |
| Warner Accountancy & Consultancy Ltd | Professional & scientific | 2024 |
| Warren's Ice Cream Limited | Hospitality | 2019 |
| Warsash Early Years Centre Ltd | Education | 2024 |
| Waterman Welding Limited | Construction | 2024 |
| Waters Estates Limited | Financial services | 2022 |
| WE ARE Viva Ltd | Professional & scientific | 2015 |
| Weatherall Consulting Ltd | Financial services | 2015 |
| Webber Holdings Limited | Financial services | 2015 |
| Webeast Ltd | Information & communication | 2012 |
| Webuyeverycar.Uk Limited | Wholesale & retail | 2025 |
| Well Rested Sleep Coach Limited | Other services | 2022 |
| Well Yachts Limited | Other services | 2025 |
| Welling Partnership LLP | | 2019 |
| Wellow Business Services Limited | Wholesale & retail | 1998 |
| Wellow Legal Limited | Professional & scientific | 2020 |
| Wellow Management Services Limited | Professional & scientific | 1998 |
| Wellow Storage Limited | Administrative & support | 2016 |
| WES & Co Ltd | Wholesale & retail | 2014 |
| Wessex Accountancy Limited | Professional & scientific | 2018 |
| Wessex Cabinet Works Limited | Manufacturing | 2013 |
| Wessex Clinical Trials Ltd | Professional & scientific | 2025 |
| Wessex Commercials (Holdings) Limited | Financial services | 2014 |
| Wessex Commercials Limited | Wholesale & retail | 2009 |
| Wessex Critical Care Training Group | Education | 2018 |
| Wessex Endocrine Solutions Limited | Health & social care | 2018 |
| Wessex Financial Limited | Professional & scientific | 2021 |
| Wessex Motorhomes Limited | Administrative & support | 2020 |
| Wessex Nichols Colorectal Limited | Health & social care | 2011 |
| Wessex Planning Ltd | Professional & scientific | 2016 |
| Wessex Protection Services (South) Limited | Administrative & support | 2019 |
| Wessex Resins & Adhesives Holdings Limited | Manufacturing | 2015 |
| Wessex Resins & Adhesives Limited | Manufacturing | 1982 |
| Wessex Skin & Laser Clinic Limited | Health & social care | 2017 |