| DRD Architectural & Interior Design Ltd | Professional & scientific | 2015 |
| Drealm Media Ltd | Information & communication | 2021 |
| Dream Desserts Retford Limited | Hospitality | 2024 |
| Dream Property Group Ltd | Real estate | 2020 |
| Drewery & Wheeldon Property Consultants LLP | | 2023 |
| Drone Defence Services Ltd | Manufacturing | 2017 |
| Drops of Juniper Limited | Manufacturing | 2018 |
| Drury Hunt Limited | Agriculture & fishing | 2010 |
| DST Automotive Ltd | Wholesale & retail | 2016 |
| Duane Hedley Properties Ltd | Real estate | 2020 |
| Duck on a Wall Ltd | Hospitality | 2024 |
| Dukeries Domestics Limited | Administrative & support | 2018 |
| Dukeries Healthcare Limited | Health & social care | 1997 |
| Dukeries Living Limited | Construction | 2023 |
| Dukeries Retreat Limited | Hospitality | 2017 |
| Durham GAS Services Limited | Construction | 2011 |
| Durham Grove Management Company Ltd | Real estate | 2026 |
| Dust & Ventilation Services Ltd | Water & waste | 2007 |
| Dvelop IT Ltd | Information & communication | 2011 |
| DW Developments (Retford) Limited | Real estate | 2016 |
| DW Joinery and Building Services Ltd | Construction | 2022 |
| Dwps (UK) Limited | Real estate | 2011 |
| E P Group Limited | Financial services | 2018 |
| E S E Landscapes Ltd | Wholesale & retail | 2010 |
| E&L Property Solutions Ltd | Real estate | 2024 |
| Earl Shilton Trials Club Ltd | Arts & recreation | 2015 |
| East Midlands Surfacing Limited | Construction | 2022 |
| Easterly Holdings Ltd | Real estate | 2018 |
| Eastfield Meadow Limited | Construction | 2021 |
| Eclipse Aerial Systems Ltd | Construction | 2016 |
| Eclipse Projects & Design Limited | Construction | 2000 |
| Eco Prime Recycling Limited | Water & waste | 2021 |
| Ecotec Engineering Limited | Construction | 2008 |
| Edgewise Edgestrip Limited | Manufacturing | 2009 |
| Education and Skills Training & Development Limited | Education | 2015 |
| Edwin Ashworth (1989) Limited | Professional & scientific | 1968 |
| Edwin Ashworth Marine Limited | Professional & scientific | 1957 |
| Edwin Ashworth Marine Supplies Limited | Professional & scientific | 1983 |
| EEJ I.T. Limited | Information & communication | 2010 |
| Effective Agile Limited | Professional & scientific | 2022 |
| Efun Coker Ltd | Professional & scientific | 2019 |
| EFW Organisation Ltd | Water & waste | 2020 |
| EJ Event Medical Limited | Health & social care | 2021 |
| Elbvs Ltd | Professional & scientific | 2016 |
| Electgrand Ltd | Hospitality | 2024 |
| Elemnnt Ltd | Professional & scientific | 2016 |
| Elite Brow and Skin Studio Ltd | Other services | 2022 |
| Elite KOI Limited | Wholesale & retail | 2016 |
| Elite Reinforcements Limited | Construction | 2022 |
| Elite Waterproofing Ltd | Construction | 2011 |
| Elliott Smith Holdings Limited | Financial services | 2023 |
| Elms Hotels Limited | Hospitality | 2019 |
| Elysian Gems UK Ltd | Wholesale & retail | 2024 |
| Em's Dog Grooming Parlour Limited | Administrative & support | 2025 |
| Embracing Emotions CIC | Education | 2024 |
| Emerald Facilities Ltd | Administrative & support | 2020 |
| Emma Lawler Ltd | Professional & scientific | 2025 |
| Empire Fire and Safety Limited | Public administration | 2006 |
| Emporium Wholesale Limited | Transport & logistics | 2023 |
| END 2 END Communications Ltd | Information & communication | 2026 |
| Eneve UK Ltd. | Administrative & support | 2011 |
| Enjoybrowns Ltd | Professional & scientific | 2020 |
| Enviro Solar Solutions Limited | Construction | 2026 |
| EPA Holdings (International) Limited | Manufacturing | 2023 |
| EPA Manufacturing Limited | Manufacturing | 2010 |
| Eqidos Limited | Financial services | 2025 |
| ER Cafe Ltd | Hospitality | 2018 |
| Es-Rentals Ltd | Real estate | 2024 |
| Eser Property Ltd | Real estate | 2025 |
| Espano Ltd | Construction | 2022 |
| ET Voila Limited | Manufacturing | 2021 |
| ETE Plant Services Limited | Manufacturing | 2017 |
| Eternal Ceremonies Limited | Other services | 2018 |
| Euro Repair & Sales (UK) Ltd | Professional & scientific | 2019 |
| Europan Limited | Transport & logistics | 1988 |
| Eurorail Crash Barriers 2000 Limited | Manufacturing | 2000 |
| Event Industry News Limited | Information & communication | 2010 |
| Eventing One Limited | Arts & recreation | 2025 |
| Events Power Solutions Ltd | Construction | 2021 |
| Evolution Community Sports Coaching CIC | Arts & recreation | 2021 |
| Evolve Restoration Limited | Administrative & support | 2025 |
| EX GEN Hydrogen Limited | Financial services | 2021 |
| Excel Furniture Agencies Ltd | Wholesale & retail | 2003 |
| Exclusive JDM Ltd | Manufacturing | 2020 |
| Extreme Performance Limited | Manufacturing | 1999 |
| Exxe Automotive Limited | Wholesale & retail | 2025 |
| Eymen Electrics Ltd | Manufacturing | 2020 |
| F C Tomlinson & SON Limited | Transport & logistics | 1988 |
| F L Green & Sons Limited | Agriculture & fishing | 2009 |
| Facial Perfection Lincoln Ltd | Other services | 2023 |
| Facility Management Services (Lincoln) Limited | Construction | 2014 |
| Fagan Safety Solutions Ltd | Other services | 2022 |
| Fagan Scaffolding Ltd | Construction | 2019 |
| Fairfield Control Systems Limited | Professional & scientific | 1994 |
| Fairfield Engineering Solutions Limited | Professional & scientific | 2019 |
| Fairfield Financial Services Limited | Financial services | 2021 |
| Fairway Carpet Centre Limited | Wholesale & retail | 2003 |
| Faith Rose Properties Limited | Real estate | 2026 |
| Family Psychology and Therapy Services Ltd | Health & social care | 2021 |
| FAN the Flames Marketing & Design Limited | Professional & scientific | 2021 |