| DSM Roofing Contractors Limited | Construction | 2012 |
| DSP4U Ltd | Construction | 2023 |
| Dtclai Limited | Information & communication | 2025 |
| Dudley Auto Detailing Limited | Administrative & support | 2025 |
| Duna Tapari Momo House Ltd | Hospitality | 2025 |
| Dyers (London) Limited | Manufacturing | 2012 |
| Dyslexia Learning and Development Limited | Education | 2016 |
| E&A Duocare Ltd | Manufacturing | 2025 |
| E&M Brothers Ltd | Construction | 2015 |
| EA Gold Company and Refineries Ltd | Mining & quarrying | 2023 |
| EA Jewellers Limited | Wholesale & retail | 2026 |
| Eahlo Ltd | Information & communication | 2026 |
| Ealdwode Limited | Professional & scientific | 2022 |
| East Broadcast & Media Services Ltd | Information & communication | 2022 |
| Eastwind Sales and Marketing Ltd | Administrative & support | 2022 |
| Easy Measure Limited | Information & communication | 2020 |
| Easygstrefunds Ltd | Information & communication | 2004 |
| EAT Pizza Ltd | Hospitality | 2024 |
| EB Mortgages Ltd | Financial services | 2026 |
| Ecit Accountants Limited | Professional & scientific | 1986 |
| Ecit Palmerston Limited | Other services | 2019 |
| Ecit Services Limited | Other services | 2019 |
| Edennn Ltd | Transport & logistics | 2024 |
| Educology Consulting Limited | Extraterritorial | 2016 |
| Educology Consulting Services Limited | Information & communication | 2016 |
| Educology Limited | Extraterritorial | 2016 |
| EGE Restaurant Limited | Hospitality | 2025 |
| EGO Nails & Spa Limited | Other services | 2025 |
| Elda Developments Limited | Real estate | 1997 |
| Electron Test Equipment Limited | Manufacturing | 2020 |
| Elisha Boateng Limited | Other services | 2025 |
| Elixir PVT Limited | Other services | 2010 |
| Elmh Properties Limited | Real estate | 2026 |
| Elyras of London Limited | Manufacturing | 2020 |
| Emi&Home Ltd | Real estate | 2025 |
| Empathy Smiles Ltd | Health & social care | 2025 |
| Empire BIS Ltd | Real estate | 2022 |
| Emylanto Tenderhands Limited | Hospitality | 2024 |
| Endoadeno Voices for Change Limited | Health & social care | 2025 |
| Ends BGS Limited | Professional & scientific | 2025 |
| Energy of Things Ltd | Professional & scientific | 2025 |
| Enfinito Global Trading Company Limited | Wholesale & retail | 2023 |
| ENL Courier Ltd | Transport & logistics | 2022 |
| Ensemble Tempus Fugit | Arts & recreation | 2017 |
| Envoyager Ltd | Extraterritorial | 2011 |
| Epic Timeless Productions Ltd | Administrative & support | 2025 |
| Equanimeous Ltd | Real estate | 2024 |
| Equita Developments Ltd | Construction | 2021 |
| Equita Investments Ltd | Real estate | 2016 |
| ESB UK Properties Limited | Real estate | 2020 |
| ESP Developments Limited | Real estate | 2013 |
| Essencego Ltd | Wholesale & retail | 2025 |
| Essential Clean Ltd | Administrative & support | 2015 |
| Essentials 24 Ltd | Wholesale & retail | 2022 |
| Ethical Property Holdings Limited | Real estate | 2016 |
| Europe Florence Limited | Extraterritorial | 2017 |
| Evasons Tape & Jointers Ltd | Construction | 2017 |
| Event-X Ltd | Administrative & support | 2000 |
| Everyday Matters Ltd | Professional & scientific | 2022 |
| Everything Available Limited | Health & social care | 2017 |
| Ewart Development Limited | Other services | 2012 |
| Exite Developments Ltd | Real estate | 2016 |
| Expressive Sounds CIC | Arts & recreation | 2023 |
| Extinguisher Warehouse Ltd | Wholesale & retail | 2012 |
| Extra Mile Plumbing Limited | Construction | 2011 |
| Eyck Consulting Ltd | Information & communication | 2023 |
| Eyebox Boutique Limited | Professional & scientific | 2015 |
| Eywa Ltd | Health & social care | 2023 |
| F.H.Q Trading Co Limited | Construction | 2020 |
| Facta Life Limited | Extraterritorial | 2010 |
| Fadak Farm Ltd | Agriculture & fishing | 2023 |
| Fairview Community Housing Association | Real estate | 2017 |
| Fairway App Limited | Information & communication | 2026 |
| Fais Consulting Limited | Professional & scientific | 2011 |
| Falke (U.K.) Limited | Wholesale & retail | 1989 |
| Family Mediation Services Ltd | Health & social care | 2018 |
| FAN Consultants Ltd | Health & social care | 2010 |
| Farzanaas Limited | Health & social care | 2026 |
| Fatiecare Limited | Health & social care | 2025 |
| Felicity Murray Ltd | Health & social care | 2021 |
| Fenor Limited | Construction | 2025 |
| Fentona Limited | Professional & scientific | 1996 |
| Ferleeshell Ltd | Manufacturing | 2023 |
| Fern Court London Limited | Real estate | 2015 |
| Fernleaf Limited | Construction | 1981 |
| Fiasco Guides Publishing Ltd | Information & communication | 2002 |
| Filentia Ltd | Information & communication | 2018 |
| Finch Support Services Ltd | Administrative & support | 2015 |
| Finley Court Foxley Lane (RTM) Limited | Real estate | 2024 |
| First Ball Cricket Academy Ltd | Other services | 2020 |
| Fiscal Direct Limited | Professional & scientific | 2023 |
| Fisher Mcdonald Ltd | Professional & scientific | 2020 |
| Fitzrovia Lettings Limited | Real estate | 2017 |
| Flagship Construction Limited | Construction | 2025 |
| Flawless Touch Limited | Wholesale & retail | 2026 |
| Flemmings Limited | Administrative & support | 2017 |
| Flocare Services Ltd | Health & social care | 2015 |
| Flogo Products Ltd | Professional & scientific | 2022 |
| Fluff Shack Ltd | Other services | 2024 |
| Flyers London Ltd | Other services | 2025 |