| Urban Sloth Ltd | Hospitality | 2025 |
| Valens Capital Limited | Financial services | 2025 |
| VAPE88 Ltd. | Wholesale & retail | 2005 |
| Vapes Prestwick Ltd | Wholesale & retail | 2026 |
| Vavasour Developments Ltd | Real estate | 2024 |
| Velocity Modular Ltd | Financial services | 2019 |
| Veracity Limited | Manufacturing | 2012 |
| Veracity UK Limited | Manufacturing | 2005 |
| Verdade Consulting Ltd | Professional & scientific | 2022 |
| Vertomi Ltd | Professional & scientific | 2001 |
| Viberta Ltd. | Professional & scientific | 2019 |
| Viberta Properties Ltd. | Real estate | 2019 |
| Vision Leisure Vehicles Ltd | Wholesale & retail | 2023 |
| Viva Garden 168 Ltd | Hospitality | 2023 |
| Viva Garden Prestwick Limited | Hospitality | 2026 |
| VP Sports Limited | Arts & recreation | 2024 |
| W Muir Property Limited | Real estate | 2018 |
| W Wilson Millar Ltd | Professional & scientific | 2014 |
| Walshaw Consulting Ltd | Administrative & support | 2006 |
| Wardwell Services Ltd | Administrative & support | 2004 |
| WAT the Cluck! Ltd | Hospitality | 2024 |
| Waverley Gallery Ltd | Wholesale & retail | 2021 |
| Webb Aviation Services Ltd | Manufacturing | 2024 |
| Wedd-Ed Ltd | Information & communication | 2023 |
| Weirgrove Limited | Health & social care | 2012 |
| Well Systems Technology Ltd | Mining & quarrying | 2011 |
| Wellsystems Management Limited | Administrative & support | 2015 |
| West Coast Catering Limited | Hospitality | 2023 |
| West Coast Hospitality Limited | Hospitality | 2020 |
| West Coast Marketing Limited | Professional & scientific | 2025 |
| West Coast Properties Limited | Real estate | 2025 |
| White Feather Company Ltd | Other services | 2002 |
| Wildflower (Bothwell) Property Ltd | Real estate | 2024 |
| Wildflower Properties (UK) Ltd | Real estate | 2024 |
| William Crawford Landscape Contractors Ltd. | Construction | 2006 |
| Wingnut Services Limited | Administrative & support | 2004 |
| Woodside Farm (Monkton) Ltd | Agriculture & fishing | 2019 |
| Working Class Productions Ltd | Information & communication | 2026 |
| Workstart Solutions Ltd | Financial services | 2026 |
| Worldwide Airways Ltd | Transport & logistics | 2024 |
| Wouldn'T Change a Thing | Education | 2018 |
| WS Energy Trading Services Limited | Administrative & support | 2010 |
| Xera Cyber Limited | Information & communication | 2026 |
| XQ Ltd | Hospitality | 2026 |
| Young Programme Events Limited | Education | 2013 |
| ZAP Products Ltd | Administrative & support | 2013 |
| ZLC Aviation Maintenance Ltd | Manufacturing | 2020 |