| DCS & Controls Limited | Administrative & support | 2011 |
| DDZ Engineering Limited | Professional & scientific | 2017 |
| DE Facto Consultants Ltd | Professional & scientific | 2014 |
| Dedicated EA Limited | Administrative & support | 2017 |
| DEE CEE Electrical Ltd | Energy | 2022 |
| Deira Collective Ltd | Hospitality | 2018 |
| Delta Pharmacy Limited | Financial services | 2025 |
| Dennis Clancy & Sons Limited | Construction | 2003 |
| Dennis Painting Ltd | Administrative & support | 2012 |
| Derbeth Safety Engineering Ltd | Administrative & support | 2001 |
| Derek Wilson Audio Limited | Information & communication | 2016 |
| Designer Angel Ltd | Wholesale & retail | 2022 |
| Deus Marine Ltd | Administrative & support | 2018 |
| Devo Group Ltd | Construction | 2026 |
| Dgpsuk Ltd | Wholesale & retail | 2023 |
| Diack Drilling Ltd | Professional & scientific | 2018 |
| Diana Hislop Research and Consultancy Limited | Professional & scientific | 2014 |
| Discover Scotland Tours Ltd. | Transport & logistics | 2006 |
| DLD Height Works Ltd | Administrative & support | 2020 |
| DMB Engineering UK Ltd | Administrative & support | 2007 |
| DMD Imports Ltd | Wholesale & retail | 2016 |
| DNG Joinery Limited | Construction | 2025 |
| Doonhome Limited | Other services | 2008 |
| DOT Nook Ltd | Information & communication | 2024 |
| DP Property Asset Group Ltd | Real estate | 2026 |
| DRM Corrosion Services Ltd | Administrative & support | 2013 |
| Drummond of Bellfield Limited | Real estate | 2020 |
| Dubber Drilling Services Ltd | Administrative & support | 2018 |
| Ductwork Services Limited | Real estate | 1993 |
| Dumbarton Road Properties Limited | Hospitality | 2007 |
| Durango Subsea Consulting Ltd | Administrative & support | 2006 |
| DW44 Ltd | Real estate | 2020 |
| Dwav Solutions Limited | Information & communication | 2012 |
| E & N Properties LLP | | 2019 |
| E a H Joinery Ltd | Construction | 2012 |
| Easywatt Ltd | Energy | 2026 |
| Ednego Petroleum Solutions Limited | Administrative & support | 2012 |
| Edugolf Limited | Education | 2024 |
| EJ Blair Engineering Ltd. | Manufacturing | 2016 |
| Electric Bakery Ltd | Hospitality | 2012 |
| Eles Electrical Services Ltd | Construction | 2024 |
| Elliot Boyce Ltd | Hospitality | 2023 |
| Elliott Architecture & Design Ltd. | Professional & scientific | 2019 |
| Elmglen Limited | Professional & scientific | 2007 |
| Elstongreen Ltd | Administrative & support | 2016 |
| Elun Hygiene Ltd | Health & social care | 2025 |
| Elxa Offshore Services Ltd | Administrative & support | 2006 |
| Emex (UK) Group Limited | Administrative & support | 2015 |
| Envirosource Consulting Limited | Administrative & support | 2006 |
| Episcope Research Service Ltd | Administrative & support | 2025 |
| Equanimity Drilling Ltd | Mining & quarrying | 2018 |
| Equine Behaviour Affiliation Ltd | Education | 2019 |
| Eripsa Group Ltd | Financial services | 2023 |
| Eripsa Investments Limited | Financial services | 2020 |
| Eripsa Limited | Real estate | 2019 |
| Esteem Care Dumfries and Galloway Limited | Health & social care | 2002 |
| Etek Europe Limited | Other services | 2007 |
| Evolve Media and Branding Hub Ltd | Professional & scientific | 2026 |
| Exordium Solutions Ltd | Information & communication | 2024 |
| Extreme Subsea Limited | Construction | 2017 |
| Failford Limited | Extraterritorial | 2023 |
| Fairway Energy Services Ltd | Construction | 2021 |
| Fast Sports Ltd. | Arts & recreation | 2009 |
| Fentech Engineering Limited | Administrative & support | 2007 |
| Ferguson Electrical Ltd | Construction | 2023 |
| Finnis Property Ltd. | Construction | 2025 |
| Fiona Lawrie Health and Fitness Ltd | Health & social care | 2025 |
| First Lift Engineers Ltd. | Manufacturing | 2004 |
| Firstax Limited | Professional & scientific | 2007 |
| Fixt Services Ltd | Administrative & support | 2024 |
| Fizzi Ltd. | Real estate | 2004 |
| Flakesdiner Limited | Hospitality | 2025 |
| Fletcher and Banks Accountants Ltd | Professional & scientific | 2022 |
| FLI FLO Mobile Ltd | Wholesale & retail | 2022 |
| Flower 2 Fruit Limited | Professional & scientific | 2019 |
| Fluffy Seal Puppies Limited | Administrative & support | 2008 |
| FLY High Early Learning & Childcare Limited | Education | 2018 |
| FNA Enterprise Ltd | Hospitality | 2023 |
| Fnaz Enterprise Ltd | Hospitality | 2025 |
| Foneshub Traders Ltd | Wholesale & retail | 2022 |
| Footstomping Leroy Scott Ltd | Information & communication | 2024 |
| Ford Offshore Services Limited | Administrative & support | 2013 |
| Fortune Star Limited | Hospitality | 2026 |
| Freelance Euro Services (Ccxxii) Limited | Administrative & support | 2001 |
| Freelance Euro Services (Cdlxxiv) Limited | Administrative & support | 2001 |
| Freelance Euro Services (Cxli) Limited | Administrative & support | 2001 |
| Freelance Euro Services (Dccci) Limited | Administrative & support | 2002 |
| Freelance Euro Services (Dcclxx) Limited | Administrative & support | 2002 |
| Freelance Euro Services (Dcxxiv) Limited | Administrative & support | 2002 |
| Freelance Euro Services (Dcxxxi) Limited | Administrative & support | 2002 |
| Freelance Euro Services (Mcccxcv) Limited | Administrative & support | 2004 |
| Freelance Euro Services (Mcccxxxix) Limited | Administrative & support | 2004 |
| Freelance Euro Services (Mcclxix) Limited | Real estate | 2004 |
| Freelance Euro Services (Mcclxxxii) Limited | Administrative & support | 2004 |
| Freelance Euro Services (Mcdlix) Limited | Administrative & support | 2005 |
| Freelance Euro Services (Mcdlv) Limited | Administrative & support | 2005 |
| Freelance Euro Services (Mcdlxxx) Limited | Administrative & support | 2005 |
| Freelance Euro Services (Mcmiv) Limited | Administrative & support | 2006 |
| Freelance Euro Services (Mdccclxi) Limited | Administrative & support | 2006 |
| Freelance Euro Services (Mdcccxcviii) Limited | Administrative & support | 2006 |