| ...WHY NOT? Inspiring Management Consultancy Ltd | Professional & scientific | 2021 |
| 1 Fones Limited | Wholesale & retail | 2026 |
| 32 Sugar & Spice Ltd | Hospitality | 2025 |
| 3D Models Scotland Limited | Professional & scientific | 2013 |
| 4-37 West Architects Ltd | Professional & scientific | 2023 |
| A Anderson Design Ltd | Administrative & support | 2006 |
| A B Munro Technical Services Limited | Administrative & support | 2006 |
| A House 2 Home Limited | Real estate | 2014 |
| A.J. Maclean Ltd | Financial services | 2018 |
| AAD Aviation Engineering Limited | Manufacturing | 2016 |
| AAK Logistics Limited | Administrative & support | 2014 |
| ABD Loler Specialists Limited | Administrative & support | 2017 |
| Aberplan Limited | Administrative & support | 2010 |
| Able Health Care Scotland Ltd | Health & social care | 2015 |
| Academy of the Inner Mind Ltd | Education | 2023 |
| Acasa Care Limited | Health & social care | 2010 |
| ACR Epos Systems Limited | Wholesale & retail | 2015 |
| Acrefern Limited | Real estate | 2005 |
| Active Care (Ayrshire) Limited | Health & social care | 2015 |
| Active Playground Management Ltd | Administrative & support | 2004 |
| Adamton Apps Ltd | Information & communication | 2023 |
| Admire Digital Limited | Administrative & support | 2013 |
| Advanced Aerial Media Ltd | Professional & scientific | 2015 |
| Aero Fastener Co. Ltd. | Transport & logistics | 1997 |
| AG Concepts Limited | Extraterritorial | 2014 |
| Ahead Property Group Limited | Real estate | 2025 |
| Air China Cargo Co. Ltd | Transport & logistics | 2026 |
| Aircraft Engineers Limited | Manufacturing | 2001 |
| Airport Driving Range Company Limited | Real estate | 1981 |
| Airport Transfers Ltd. | Extraterritorial | 2005 |
| Aitken Ventures Limited | Hospitality | 2026 |
| AJS Cafe Ltd | Hospitality | 2025 |
| AK Offshore Technology Limited | Administrative & support | 2017 |
| Alex White Consulting Limited | Professional & scientific | 2024 |
| Alianna Consulting Limited | Administrative & support | 2005 |
| ALL About Barrhead Limited | Other services | 2016 |
| ALL Generations Ltd | Financial services | 2016 |
| Allander Homecare Ltd | Health & social care | 2009 |
| Allenwest Group Limited | Manufacturing | 2010 |
| Allenwest Limited | Manufacturing | 2026 |
| Allenwest Wallacetown Limited | Manufacturing | 1999 |
| Alloway Estates Limited | Real estate | 2013 |
| Almond Blossom Ltd. | Wholesale & retail | 2004 |
| Alysida Property Investments Ltd | Real estate | 2019 |
| Amdem Design Ltd | Administrative & support | 2013 |
| Andmar Property Limited | Real estate | 1962 |
| Andrew Kent Software Ltd. | Information & communication | 2010 |
| Anfield Systems Limited | Administrative & support | 2007 |
| Antaeus Energy Ltd | Real estate | 2009 |
| Anxiety Stress Life Awareness CIC | Health & social care | 2024 |
| AP Business Support Limited | Administrative & support | 2019 |
| Apex Preservation Solutions Ltd | Construction | 2025 |
| Applink Ltd | Information & communication | 2022 |
| Apprenticeship Bootcamp (Scotland) Limited | Education | 2024 |
| Apprenticeship Bootcamp Ltd | Education | 2021 |
| Ardayre Bridge Consulting Limited | Professional & scientific | 2025 |
| Arden Capital Ltd | Professional & scientific | 2012 |
| Ardmore Consultants Ltd | Financial services | 2012 |
| Ardrossan Storage Company Limited | Extraterritorial | 2022 |
| ARG Management Consulting Ltd | Other services | 2017 |
| Arloandjude Limited | Wholesale & retail | 2019 |
| ARM Homes Limited | Construction | 2016 |
| Armour Home Improvements Ltd | Construction | 2020 |
| Armstrong HR Ltd | Administrative & support | 2015 |
| Artisan Coatings Ltd | Construction | 2018 |
| Aryanna Ltd | Hospitality | 2023 |
| Asbestos Analytical Services Limited | Administrative & support | 2011 |
| Ashdelle Design Consultancy Limited | Administrative & support | 2006 |
| Ashleigh (Scotland) Limited | Construction | 2001 |
| Ashley Property Group Ltd | Real estate | 2018 |
| Atom Create (Scotland) Limited | Professional & scientific | 2007 |
| Aurah Medspa Ltd | Other services | 2025 |
| Autocad Detailing Services Limited | Professional & scientific | 2006 |
| Avid Scot Consulting Ltd | Professional & scientific | 2025 |
| AVT Prestwick Limited | Wholesale & retail | 2022 |
| Awesome Project Management Limited | Administrative & support | 2016 |
| AYR & District Flower Show | Arts & recreation | 2017 |
| AYR Borne Ltd | Wholesale & retail | 2026 |
| Ayrshire & Arran Golf Partnership Ltd | Hospitality | 2014 |
| Ayrshire Agricultural Association | Agriculture & fishing | 2006 |
| Ayrshire Cycle Services Ltd | Wholesale & retail | 2023 |
| Ayrshire Discount Furniture Limited | Wholesale & retail | 2007 |
| Ayrshire Fencing & Gardening Limited | Administrative & support | 2026 |
| Ayrshire Golf Tours Limited | Administrative & support | 2006 |
| Ayrshire K9 Play Park Ltd | Other services | 2015 |
| Ayrshire Plastering Services Limited | Construction | 2010 |
| Ayrshire Steels (Stockholders) Limited | Construction | 2008 |
| Ayrshire Steels Limited | Construction | 1987 |
| Ayrshire Training Services ATS Limited | Professional & scientific | 2022 |
| B Shanks Joinery and Building Services Limited | Construction | 2014 |
| B.Read Engineers Limited | Administrative & support | 2013 |
| B4ASIA Ltd | Professional & scientific | 2013 |
| Balmoral Homecare Ltd | Health & social care | 2005 |
| Barlure Farmers Limited | Agriculture & fishing | 2008 |
| Barral UK Neural Ltd | Health & social care | 2011 |
| BC Motorhomes & Leisure Ltd | Wholesale & retail | 2019 |
| BCM Technical Services Limited | Administrative & support | 1996 |
| Beau Beau Boutique Limited | Wholesale & retail | 2016 |
| Bek---Audio Limited | Information & communication | 2010 |
| Belmont Business Development Solutions Ltd | Administrative & support | 2009 |