| Elizabeth House Management Company (Waterlooville) Limited | Households | 2004 |
| Elkoni Search Limited | Administrative & support | 2023 |
| Ellar UK Ltd | Real estate | 2016 |
| Ellens Care Limited | Health & social care | 2014 |
| Elleva Consulting Limited | Information & communication | 2023 |
| Elliott Dugan Holdings Limited | Professional & scientific | 2020 |
| ELM & LRM Property Ltd | Real estate | 2025 |
| Elmdene International Limited | Manufacturing | 1963 |
| Elmdene Investments Limited | Professional & scientific | 1988 |
| Elmdene Limited | Manufacturing | 2005 |
| Elonxi Ltd | Manufacturing | 2013 |
| Elroi Healthcare Limited | Health & social care | 2024 |
| Elseandor Limited | Information & communication | 2016 |
| Elta Trading Ltd | Wholesale & retail | 2023 |
| Emad Limited | Hospitality | 2018 |
| Emallex Engineering Solutions UK Ltd | Professional & scientific | 2015 |
| Eman Spice Limited | Hospitality | 2022 |
| Emari Group Limited | Professional & scientific | 2017 |
| Embee Consultancy Ltd | Information & communication | 2022 |
| Ember and Iron Ltd | Wholesale & retail | 2025 |
| Embrace Associates Ltd | Administrative & support | 2023 |
| Emch Properties Ltd | Real estate | 2016 |
| Emerald SIX Limited | Wholesale & retail | 2020 |
| Emeraldec Ltd | Construction | 2025 |
| Emerge Enterprise Limited | Real estate | 2016 |
| Emergency Plumber Hampshire Ltd | Other services | 2017 |
| Emilys Holdings Ltd | Real estate | 2015 |
| Emkay Retail Ltd | Wholesale & retail | 2023 |
| Emma Staley Photography Ltd | Professional & scientific | 2026 |
| Emmabrook Court Limited | Households | 1978 |
| Emmerson Estates Ltd | Real estate | 2018 |
| Emmerson Transport Ltd | Transport & logistics | 2012 |
| Emmytex Company Ltd | Health & social care | 2026 |
| Empire 888 Ltd | Hospitality | 2024 |
| Empire Assets and Services Ltd | Real estate | 2024 |
| Empire Commercial Contractors Ltd | Construction | 2025 |
| Empire Electrical Services Ltd | Construction | 2016 |
| Empire Partitions Ltd | Construction | 2021 |
| Empower Global | Education | 1995 |
| Empower ME Recruitment Ltd | Health & social care | 2019 |
| Empowered Global Ltd | Education | 2022 |
| Empoweredoasiscaregroup Ltd | Public administration | 2026 |
| Empowerment Housing Project CIC | Hospitality | 2025 |
| Emsworth Properties Ltd | Real estate | 2016 |
| EMZ World Official Ltd | Wholesale & retail | 2026 |
| Enable Ability | Health & social care | 1978 |
| Enamorada Global Logistics Ltd | Transport & logistics | 2026 |
| Encore Hair Studio Ltd | Other services | 2024 |
| Encounter Nature Limited | Administrative & support | 2023 |
| Ency Associates Ltd | Professional & scientific | 2023 |
| Endurance Racing Legends Ltd | Other services | 2017 |
| Enegen Power Systems Limited | Information & communication | 2012 |
| Energise IT Company Ltd | Information & communication | 2022 |
| Energy CDM Ltd | Professional & scientific | 2020 |
| Energy Cooling Limited | Energy | 2012 |
| Energy Saving and Environment Ltd | Transport & logistics | 2015 |
| Energy Saving Enterprise Limited | Other services | 2020 |
| Engage Digital (Saas) Ltd | Information & communication | 2019 |
| Engineered Marine Systems Ltd | Professional & scientific | 2017 |
| Engineering and Management Projects Ltd | Professional & scientific | 2014 |
| England and Wales Blind Golf | Arts & recreation | 1997 |
| Enhance Decor Limited | Construction | 2001 |
| Enhanced Floors Limited | Administrative & support | 2011 |
| Enjay Electrical Limited | Energy | 2007 |
| Enki Projects Ltd | Construction | 2024 |
| ENL Group Limited | Financial services | 2000 |
| ENL Limited | Manufacturing | 1958 |
| ENL Performance Limited | Manufacturing | 2025 |
| Enmac Building Contractors Limited | Construction | 2011 |
| Ennis & Co Group Limited | Administrative & support | 2021 |
| Ennis & Co Ltd | Administrative & support | 2011 |
| Ensign (GB) Limited | Education | 2004 |
| Ensign Commerce Limited | Real estate | 2017 |
| Ensign Enterprises Limited | Extraterritorial | 2012 |
| Ensign Management Limited | Real estate | 2015 |
| ENT Plus Limited | Health & social care | 2013 |
| Enterprise MOT Limited | Wholesale & retail | 2021 |
| Entrepreneur Meet Ltd | Administrative & support | 2023 |
| Entrepreneurs Unite Social Enterprise Limited | Professional & scientific | 2015 |
| ENV Surveys Limited | Professional & scientific | 2002 |
| Environment Films Ltd | Information & communication | 2003 |
| Environmental Projects Southern Limited | Construction | 2010 |
| Enviropro Limited | Extraterritorial | 1995 |
| Envisage Technology Limited | Other services | 1999 |
| Eoph Limited | Education | 2016 |
| Epaa (2016) Limited | Wholesale & retail | 2016 |
| EPB Flooring Ltd | Construction | 2013 |
| EPC Easy Limited | Administrative & support | 2014 |
| EPC Energy Wise Ltd | Professional & scientific | 2026 |
| Epgrw Limited | Real estate | 2024 |
| Epiphany Developments Limited | Wholesale & retail | 2015 |
| Epmc (UK) Limited | Extraterritorial | 2009 |
| Epsilon Associates Limited | Real estate | 2018 |
| Equation Management Limited | Professional & scientific | 2009 |
| Equilibrium Careers Ltd | Education | 2025 |
| Equity Cleaning Services Limited | Administrative & support | 1977 |
| Equon 8 Ltd | Manufacturing | 2016 |
| ERB Projects Limited | Other services | 2020 |
| Erccs Management Ltd | Professional & scientific | 2024 |
| Erev Ltd | Administrative & support | 2012 |