| Urbatopia Ltd | Construction | 2017 |
| Usman Sultan Ltd | Wholesale & retail | 2025 |
| Utably Ltd | Information & communication | 2025 |
| UTC Portsmouth | Education | 2014 |
| Utilidex Ltd. | Information & communication | 2011 |
| Utility Delta Business Services Limited | Construction | 2021 |
| Utility Project Partners Limited | Professional & scientific | 2026 |
| Utopia Asset Management Ltd | Financial services | 2015 |
| Utopia Technologies Limited | Information & communication | 2017 |
| Uu-U Design Limited | Arts & recreation | 2014 |
| Uume Limited | Information & communication | 2020 |
| V & E Services (South) Limited | Professional & scientific | 2023 |
| V & N Associates Ltd | Real estate | 2016 |
| V N Mitrev Limited | Health & social care | 2011 |
| V&D Living Spaces Ltd | Real estate | 2026 |
| V-Race Limited | Construction | 2005 |
| V.& S. (NO.6) Limited | Professional & scientific | 1987 |
| V.Roman Ltd | Wholesale & retail | 2019 |
| V1 Aero Engineering Ltd | Manufacturing | 2019 |
| V3 Compliance Solutions Limited | Construction | 2004 |
| V3 Contract Services Limited | Construction | 2016 |
| V3 Recruitment Limited | Administrative & support | 2008 |
| V8 Gym Limited | Arts & recreation | 2025 |
| Valcor Haulage Ltd | Transport & logistics | 2023 |
| Vale Southern Construction Limited | Construction | 1994 |
| Vale Southern Holdings Ltd | Professional & scientific | 2005 |
| Vale Southern Limited | Construction | 2025 |
| Valente Hair Limited | Other services | 2016 |
| Valeo Capital UK Ltd | Professional & scientific | 2020 |
| Vales Group Limited | Financial services | 2018 |
| Valimirauta Limited | Construction | 2016 |
| Valleybrook Ltd | Construction | 2019 |
| Valpone Engineering Ltd | Professional & scientific | 2020 |
| Valtrix Systems Limited | Professional & scientific | 2025 |
| Valytica Ltd | Information & communication | 2020 |
| Van View Ltd | Manufacturing | 2025 |
| Vanamo Ltd | Wholesale & retail | 2024 |
| Vang Consultants Ltd | Information & communication | 2021 |
| Vanguard Construction Group Ltd | Construction | 2026 |
| Vanguard Electrical Compliance Limited | Professional & scientific | 2026 |
| Vanilla Gorilla Art Limited | Other services | 2021 |
| Vanilla Moon Ltd | Other services | 2009 |
| Vantari Holdings Ltd | Financial services | 2025 |
| Vantari Swan Ltd | Construction | 2026 |
| Vantari Woodgate Ltd | Construction | 2026 |
| VAP Medical Ltd | Health & social care | 2021 |
| Vape Shop (Southsea) Ltd | Wholesale & retail | 2009 |
| Vapertrails Portsmouth Limited | Wholesale & retail | 2015 |
| Vard Construction Ltd | Construction | 2021 |
| Varga Dominique Limited | Transport & logistics | 2019 |
| Varis 01 Ltd | Hospitality | 2025 |
| Vasagam Ltd | Wholesale & retail | 2025 |
| Vasco Healthcare Limited | Health & social care | 2017 |
| Vaughan Design and Development Limited | Construction | 2019 |
| Vavi Properties Limited | Real estate | 2025 |
| Vayo Logistics Ltd | Transport & logistics | 2017 |
| VB UK Holdings Limited | Real estate | 2017 |
| VBN Properties Limited | Real estate | 2016 |
| VCS Physiotherapy Ltd | Health & social care | 2025 |
| VDK Garage Ltd | Wholesale & retail | 2026 |
| Vectcare Ltd | Health & social care | 2024 |
| Veesupportservices 247 Ltd | Administrative & support | 2023 |
| Veesupportservices Ltd | Administrative & support | 2020 |
| Vega VMN Limited | Real estate | 2022 |
| Veggiepets.Com Limited | Wholesale & retail | 2018 |
| Vel-S Enterprise Limited | Transport & logistics | 2015 |
| Velocetec Holdings Ltd | Financial services | 2022 |
| Velocetec Ltd | Professional & scientific | 2018 |
| Velociai Ltd | Information & communication | 2025 |
| Velvet Queen Ltd | Wholesale & retail | 2016 |
| Vendors and Buyers Ltd | Real estate | 2018 |
| Venetian Estates Limited | Households | 2013 |
| Venew Limited | Professional & scientific | 2014 |
| Venom Championship Wrestling Limited | Arts & recreation | 2023 |
| VENTIL8ED Limited | Construction | 2019 |
| Ventura Vending Ltd | Wholesale & retail | 2025 |
| Venture Homes Southern Ltd | Construction | 2025 |
| Verde Capital Limited | Extraterritorial | 2022 |
| Verdora Consulting Limited | Professional & scientific | 2017 |
| Veritas Laboratory Services Limited | Professional & scientific | 2022 |
| Verlox Ltd | Information & communication | 2026 |
| Vernon House Freehold Company Limited | Real estate | 2002 |
| Veronica Properties Ltd | Real estate | 2024 |
| Verrecchia Limited | Hospitality | 2013 |
| Versi Asset Management Limited | Real estate | 2022 |
| Vertex Workforce Ltd | Administrative & support | 2025 |
| Verticalsoft Limited | Information & communication | 2006 |
| Verve HR Limited | Other services | 2017 |
| Vespasian Security Limited | Administrative & support | 2006 |
| Vestal Developments Limited | Construction | 2005 |
| Vesti.Ai Ltd | Information & communication | 2018 |
| Veyra 22 Limited | Real estate | 2026 |
| VIA Business Consulting Ltd | Professional & scientific | 2018 |
| Viable Digital Limited | Information & communication | 2021 |
| Vibrant Cart UK Ltd | Wholesale & retail | 2025 |
| Vibrant Data Systems Limited | Information & communication | 1996 |
| Viceroy Court (Petersfield) Limited | Other services | 2014 |
| Vickers Trading Ltd | Extraterritorial | 2022 |
| Victoria Claridge Limited | Professional & scientific | 2015 |
| Victoria House Management (Portsmouth) Limited | Households | 1987 |