| Empire Club Limited | Hospitality | 2021 |
| Empire Flow Services Ltd | Construction | 2025 |
| Empire Paint and Body Ltd | Wholesale & retail | 2016 |
| Empiric Partners LLP | | 2017 |
| Employer Branding Made Easy Limited | Education | 2020 |
| Empowered Marketing UK Ltd | Professional & scientific | 2025 |
| Empreendimentos RD Limited | Wholesale & retail | 2025 |
| Empyrean Source Ltd | Information & communication | 2026 |
| Empyrion Information Governance Ltd | Information & communication | 2026 |
| Enact Arts CIC | Education | 2024 |
| ENB Holdings Ltd | Financial services | 2025 |
| END2END Property Services Limited | Real estate | 2026 |
| Endeavour Property Group Limited | Extraterritorial | 2021 |
| Endeavour Southern Limited | Administrative & support | 2014 |
| Endgame Limited | Real estate | 2025 |
| Endon Lighting Limited | Wholesale & retail | 1998 |
| Endspec Limited | Wholesale & retail | 2022 |
| Enduosports Ltd | Manufacturing | 2019 |
| Endure Global Ltd | Information & communication | 2025 |
| Energianova Ventures (UK) Ltd | Other services | 2025 |
| Energizer Hub Ltd | Wholesale & retail | 2025 |
| Energy BU Ltd | Energy | 2014 |
| Energy Design Holdings Limited | Financial services | 2020 |
| Energy G Ltd | Energy | 2014 |
| Energy Helper App Ltd | Information & communication | 2026 |
| Energy L Ltd | Energy | 2014 |
| Energycount Limited | Construction | 2007 |
| Energylevelz Limited | Construction | 2025 |
| Enerlog Ltd | Wholesale & retail | 2021 |
| Enew Ltd | Manufacturing | 2019 |
| Engage Development Programmes Ltd | Professional & scientific | 2007 |
| Engineering Partners (Southampton) LLP | | 2008 |
| Enginuity | Education | 1988 |
| Enginuity Skills Limited | Education | 2019 |
| English Country Schools Limited | Education | 2006 |
| English Court Management Limited | Households | 2000 |
| Enkai Consulting Ltd | Information & communication | 2017 |
| Enkai Properties Ltd | Real estate | 2021 |
| Enkai Trade Ltd | Wholesale & retail | 2022 |
| Enliven Digital Solutions Ltd | Information & communication | 2020 |
| Enlumber Ltd | Wholesale & retail | 2026 |
| Ennerdale Bridge Ltd | Professional & scientific | 2017 |
| Ennovic Technology Limited | Wholesale & retail | 2022 |
| Ensemble Consultancy Management Limited | Professional & scientific | 2024 |
| ENSI14 Limited | Hospitality | 2021 |
| Ensor Estates Property Ltd | Real estate | 2017 |
| Entador Systems Limited | Extraterritorial | 2018 |
| Entech (Poole) Limited | Professional & scientific | 1994 |
| Enterprise Express Limited | Transport & logistics | 1995 |
| Enterprise Intelligence Ltd | Information & communication | 2006 |
| Enterprise RFF Limited | Wholesale & retail | 2025 |
| Entserv Information Security UK Ltd | Information & communication | 2023 |
| Envio Studio Ltd | Professional & scientific | 2023 |
| Envionics Ltd | Professional & scientific | 2016 |
| Envirodiamond Limited | Manufacturing | 2020 |
| Environment and Energy Limited | Professional & scientific | 2007 |
| Environmental Chemistry Testing Limited | Professional & scientific | 2006 |
| Envista Branding Ltd | Wholesale & retail | 2006 |
| Envista Group Ltd | Professional & scientific | 2016 |
| Envista Ltd | Professional & scientific | 2016 |
| Envista Media Ltd | Professional & scientific | 2017 |
| Envolve Property Buyers Ltd | Real estate | 2025 |
| EO Careers Ltd | Administrative & support | 2025 |
| EON Properties Limited | Real estate | 2017 |
| EPC 24 Limited | Administrative & support | 2024 |
| Epic Endurance CIC | Other services | 2024 |
| Epic Road Rides Ltd | Information & communication | 2021 |
| EPN Media Ltd | Information & communication | 2021 |
| Eprivate Ltd | Wholesale & retail | 2025 |
| Eqodata Limited | Information & communication | 2019 |
| Equalearn Ltd. | Professional & scientific | 2021 |
| Equals Five Ltd | Other services | 2022 |
| Equanimity Property Ltd | Real estate | 2023 |
| Equation Accounting Ltd | Professional & scientific | 2008 |
| Equator Group Limited | Administrative & support | 2018 |
| Equestrian Super Cards Limited | Wholesale & retail | 2022 |
| Equidria Limited | Professional & scientific | 2023 |
| Equilibrium Management Services Ltd | Professional & scientific | 2025 |
| Equip-Able Limited | Manufacturing | 2007 |
| Equity Analytics Limited | Financial services | 2015 |
| Equity Results Limited | Professional & scientific | 2018 |
| Equity Track Investments Limited | Financial services | 2012 |
| Ercilla Solutions Limited | Professional & scientific | 2007 |
| Erciyess Limited | Hospitality | 2018 |
| ERF Consultants Ltd | Professional & scientific | 2025 |
| Erigena Ltd | Information & communication | 2007 |
| Erik Čižmár Ltd | Extraterritorial | 2024 |
| Erika Bodgan Limited | Health & social care | 2017 |
| Erlc Services Ltd | Wholesale & retail | 2015 |
| Ermine Consulting Ltd | Professional & scientific | 2018 |
| Ernest Knifton,Limited | Real estate | 1923 |
| Ernesto Castro Ltd | Other services | 2025 |
| Erosivity Limited | Other services | 2025 |
| Errington Stanley Mortgages Ltd | Financial services | 2017 |
| Erudite Partners Ltd | Professional & scientific | 2022 |
| Erudity Ltd | Information & communication | 2026 |
| Eryx Auto Styling Ltd | Wholesale & retail | 2024 |
| ES Marine Limited | Manufacturing | 2013 |
| ES Vedra Limited | Administrative & support | 2017 |
| Es.Composite Ltd | Professional & scientific | 2026 |