| Upstream Partners International Limited | Professional & scientific | 2025 |
| Upton Electrics Ltd | Construction | 2010 |
| Upton Home Hardware Limited | Wholesale & retail | 1996 |
| Uptown Mini Ltd | Wholesale & retail | 2006 |
| Uptoyou Ltd | Information & communication | 2025 |
| Upward Tech IO Ltd | Wholesale & retail | 2025 |
| UR Badokki Estore Ltd | Wholesale & retail | 2025 |
| Urban & Country Homes Ltd | Extraterritorial | 2011 |
| Urban Edge Modular Ltd | Construction | 2025 |
| Urban Gear Ltd | Information & communication | 2019 |
| Urban Indi Limited | Hospitality | 2026 |
| Urban Living Estates Ltd | Hospitality | 2025 |
| Urban Nest Stays Limited | Real estate | 2024 |
| Urban Nomad Camden Limited | Real estate | 2025 |
| Urban Nomad Camden NO.2 Limited | Hospitality | 2026 |
| Urban Nomad Operations Camden Limited | Hospitality | 2025 |
| Urban Smart Ltd | Information & communication | 2016 |
| Urbane World Limited | Wholesale & retail | 2017 |
| Urights for Africa Ltd | Professional & scientific | 2025 |
| Urimd Limited | Wholesale & retail | 2025 |
| Urraca Property Limited | Real estate | 2024 |
| Usal Brighton Ltd | Other services | 2024 |
| USE Signal Marketing Ltd | Wholesale & retail | 2025 |
| USH One Ltd | Real estate | 2024 |
| Usmleorbit Ltd | Wholesale & retail | 2025 |
| Usuda Ltd | Information & communication | 2013 |
| Usurph Limited | Education | 2026 |
| UTD & Cole Limited | Financial services | 2015 |
| Utility Advice Bureau Limited | Administrative & support | 2006 |
| Utility Advice Bureau.Co.Uk Ltd | Professional & scientific | 2008 |
| UVE Media Solutions Ltd | Professional & scientific | 2025 |
| Uvora Ltd | Wholesale & retail | 2026 |
| UX Architects Ltd | Professional & scientific | 2019 |
| UX Media Limited | Extraterritorial | 2007 |
| Uxen Limited | Information & communication | 2015 |
| UZAIRAHMED4864 Ltd | Wholesale & retail | 2020 |
| V & T Nails & Beauty Ltd | Other services | 2023 |
| V C Dominey Electrical Limited | Extraterritorial | 2003 |
| V Cybersec Ltd | Information & communication | 2022 |
| V J Bransom Limited | Administrative & support | 2015 |
| V L (Imports) Ltd | Wholesale & retail | 2014 |
| V L Parkstone Ltd | Wholesale & retail | 2014 |
| V Physio Phyle Ltd | Professional & scientific | 2020 |
| V Safety Limited | Wholesale & retail | 2016 |
| V&L Market Ltd | Wholesale & retail | 2025 |
| V&M Living Ltd | Health & social care | 2024 |
| V-Build Construction Ltd | Construction | 2017 |
| V.C. Dominey Electrical Services Limited | Construction | 1993 |
| V360 Marketing Ltd | Information & communication | 2024 |
| V88 Holdings Ltd | Financial services | 2025 |
| V88 Limited | Information & communication | 2022 |
| VA Branding Limited | Administrative & support | 2023 |
| VA Parkstone Limited | Wholesale & retail | 2014 |
| VA VA Vegan Limited | Hospitality | 2017 |
| Vac-Line Ltd | Extraterritorial | 2023 |
| Vadim Solutions Ltd | Wholesale & retail | 2026 |
| Vagovitae Ltd | Professional & scientific | 2025 |
| Vagrants, Vagabonds, and Villains Ltd | Professional & scientific | 2018 |
| Valcarni Ltd | Real estate | 2017 |
| Vale of Glamorgan Brewery Limited | Manufacturing | 2004 |
| Valegate Limited | Real estate | 2011 |
| Valenteo Limited | Health & social care | 2020 |
| Valentine Court Management Limited | Households | 1997 |
| Valerii Veselov Ltd | Transport & logistics | 2025 |
| Valiant Homes Group Limited | Real estate | 2025 |
| Valley Motors Limited | Real estate | 1986 |
| Valleyplus Limited | Other services | 2026 |
| Vallway Ltd | Hospitality | 2026 |
| Valona Ltd | Hospitality | 2021 |
| Valorwatch Security Solutions Ltd | Administrative & support | 2025 |
| Valstera Property Limited | Real estate | 2021 |
| Value Insight Limited | Information & communication | 2017 |
| Value Products Limited | Wholesale & retail | 1995 |
| Valuetransform Ltd | Professional & scientific | 2025 |
| Valuxe Ltd | Wholesale & retail | 2025 |
| Vana Shop Ltd | Wholesale & retail | 2026 |
| Vandelay Solutions Ltd | Agriculture & fishing | 2014 |
| Vanguard Kpital Ltd | Real estate | 2025 |
| Vanguard Nutraceuticals Limited | Wholesale & retail | 2024 |
| Vanguard Revenue Consultants Limited | Professional & scientific | 2026 |
| Vanilla H&B Ltd | Other services | 2025 |
| Vanilla Hair and Beauty Ltd | Other services | 2005 |
| Vant Direct Ltd | Wholesale & retail | 2026 |
| Vantage Composites Limited | Manufacturing | 2012 |
| Vantage Foods Limited | Manufacturing | 1994 |
| VANTAGE4 Limited | Professional & scientific | 2025 |
| Vantagex Global Limited | Wholesale & retail | 2025 |
| Vantaswan Ltd | Construction | 2025 |
| VAP Digital Business Ltd | Wholesale & retail | 2025 |
| VAP3HUB Ltd | Wholesale & retail | 2025 |
| Vapepav Ltd | Wholesale & retail | 2016 |
| Vaperstar Poole Ltd | Wholesale & retail | 2018 |
| Varenyam Limited | Wholesale & retail | 2021 |
| Varish Properties Investment Ltd | Real estate | 2024 |
| Varo Construction Ltd | Professional & scientific | 2014 |
| Varo Consulting Ltd | Professional & scientific | 2012 |
| Vasu Circle Ltd | Financial services | 2023 |
| Vaughan Management Solutions Limited | Professional & scientific | 2003 |
| Vault Coaching Ltd | Other services | 2020 |
| Vault Properties Ltd | Real estate | 2025 |