| Eaves Rail Ltd | Construction | 2014 |
| Eazy Movers Logistics and Freight Limited | Transport & logistics | 2023 |
| EC Cycles Limited | Wholesale & retail | 2016 |
| ECD EOT Limited | Financial services | 2022 |
| Ecoach Ltd. | Education | 2017 |
| Ecoc Trading Co., Ltd | Wholesale & retail | 2024 |
| Ecoperi Ltd | Manufacturing | 2026 |
| Ecorevive Computers Ltd | Manufacturing | 2025 |
| EDGE2EDGEROOFING&MAINTENANCE Ltd | Construction | 2025 |
| Edu-Dat Ltd | Education | 2024 |
| Effective Holdings Ltd | Financial services | 2025 |
| Effective Human Resource Management Limited | Administrative & support | 2011 |
| Effective PAY Solutions Limited | Professional & scientific | 2003 |
| Effective Payroll Solutions Ltd | Administrative & support | 2016 |
| Eggy Ltd | Transport & logistics | 2025 |
| Eira-Wen Limited | Hospitality | 2026 |
| EL Reporting Limited | Professional & scientific | 2023 |
| EL Sports Ltd | Arts & recreation | 2023 |
| Electro-Main Services Ltd | Construction | 2016 |
| Electrotech Electrical Engineering Limited | Construction | 2019 |
| Elevation Solutions Ltd | Professional & scientific | 2026 |
| ELH & Partners Limited | Professional & scientific | 2012 |
| Elisabeth Bucknall Properties Limited | Real estate | 2024 |
| Elite Development and Letting Limited | Real estate | 2010 |
| Elite Mart Holdings Ltd | Wholesale & retail | 2025 |
| Elite Underground Engineering Solutions Limited | Construction | 2023 |
| Elley Properties Ltd | Households | 2024 |
| ELM Business Analysis Limited | Information & communication | 2025 |
| ELM Property Rental Limited | Real estate | 2017 |
| Elmwood Saundersfoot (Flats Management) Limited | Real estate | 1978 |
| Elston Barber Ltd | Other services | 2025 |
| Elyn Boutique Ltd | Arts & recreation | 2020 |
| Elysian South Wales Ltd | Other services | 2021 |
| EM Proper-Tŷ Buyers Ltd | Real estate | 2019 |
| Emma Louise Luxe Ltd | Professional & scientific | 2025 |
| Emma Maddock Properties Ltd | Real estate | 2022 |
| Empathy Support Ltd | Health & social care | 2018 |
| Emtage Rail Ltd | Construction | 2023 |
| Encompass Care (Wales) Limited | Health & social care | 2005 |
| Endeavour Property Developers Ltd | Construction | 2022 |
| Endless Road Garage Limited | Wholesale & retail | 2018 |
| Energy Elements Ltd | Wholesale & retail | 2025 |
| Enhance Clinics UK Limited | Wholesale & retail | 2026 |
| Enica Ltd | Professional & scientific | 2006 |
| Enigma Interiors Ltd | Construction | 2016 |
| Ennyah Digital Technology Shenzhencollc Ltd | Wholesale & retail | 2026 |
| Enterprise College Wales Limited | Education | 1995 |
| ENTERPRISE85 Ltd | Transport & logistics | 2022 |
| Envirobuild Property Solutions Ltd | Construction | 2014 |
| Environmental Compliance Limited | Professional & scientific | 2001 |
| EP Technology Co., Limited | Professional & scientific | 2014 |
| EPR Experts Ltd | Manufacturing | 2015 |
| Eqcriwio Ltd | Administrative & support | 2025 |
| Equity SIX Ltd | Real estate | 2024 |
| ERG Construct Ltd | Construction | 2021 |
| Erica Henderson Locum Services Ltd | Health & social care | 2024 |
| Erthold Ltd | Information & communication | 2018 |
| Erynn James Aesthetics Ltd | Other services | 2023 |
| Estatecatering Limited | Hospitality | 2022 |
| Esterling Developments Limited | Construction | 2022 |
| Euahone Ltd | Wholesale & retail | 2025 |
| Euphrates Barber Limited | Other services | 2026 |
| Evagreen Consulting Limited | Professional & scientific | 2012 |
| Evanis Ltd | Construction | 2025 |
| Evans Family Investments Ltd | Real estate | 2024 |
| Eventclean Limited | Arts & recreation | 2014 |
| Evertherm Limited | Construction | 2026 |
| Evion Auto Solutions (2018) Limited | Wholesale & retail | 2018 |
| Evolve Motor Company Ltd | Wholesale & retail | 2017 |
| Evopoint Limited | Information & communication | 2026 |
| Ewis Engineering Consultants Ltd | Wholesale & retail | 2025 |
| Ewood International Ltd | Wholesale & retail | 2025 |
| Excell Services - AO Ltd | Construction | 2025 |
| Exhara Luma CIC | Professional & scientific | 2025 |
| Express Design & Print Limited | Manufacturing | 2006 |
| Exterior Cleans Ltd | Administrative & support | 2025 |
| EYE Contact EYE Care Limited | Health & social care | 2008 |
| Eyewear Resources Ltd | Wholesale & retail | 2020 |
| Eyrie Down Limited | Administrative & support | 2012 |
| Fabby Dabby Ltd | Wholesale & retail | 2013 |
| Face Studios Limited | Information & communication | 2020 |
| Facelift Property Ltd | Real estate | 2025 |
| Faceyrailservices Limited | Construction | 2019 |
| Failure Creative Ltd | Professional & scientific | 2019 |
| Fairway Estates Limited | Construction | 2014 |
| Fairytale Carriages Limited | Transport & logistics | 2016 |
| Faith Rail Limited | Construction | 2020 |
| Falcon Carpentry & Building Limited | Construction | 2020 |
| Falcon SEC 15 Ltd | Administrative & support | 2017 |
| FAN Life Apparel Ltd | Wholesale & retail | 2024 |
| Fancy Box Ltd | Wholesale & retail | 2024 |
| Fangqia Limited | Wholesale & retail | 2023 |
| Faraday Watt Ltd | Construction | 2025 |
| Farway Industrial Co., Ltd | Professional & scientific | 2014 |
| FAT Head Productions Ltd | Information & communication | 2024 |
| Fayora Ltd | Wholesale & retail | 2025 |
| FB Training and Media Ltd | Information & communication | 2025 |
| Federation of Recorded Music Societies Limited | Other services | 1961 |
| Feet in Focus (Wales) Limited | Health & social care | 2012 |
| Feldestiny Resources Limited | Health & social care | 2025 |