| E.L.I.T.E. Supported Employment Agency Ltd | Administrative & support | 1994 |
| Eagle Contractors Ltd | Construction | 2019 |
| EAN Construction Services Ltd | Construction | 2019 |
| Earthmonkey Media Limited | Manufacturing | 2007 |
| East Vale Accounts Ltd | Real estate | 2022 |
| East Vale Residents Company Limited | Households | 1999 |
| Eco-Fire Limited | Public administration | 2023 |
| Ecobunker Limited | Administrative & support | 2014 |
| Ecofit Energy Systems Ltd | Construction | 2011 |
| Ecoglaze U.K. Limited | Construction | 2017 |
| Ecostructive Ltd | Construction | 2017 |
| Eden Rose Aesthetics Ltd | Other services | 2021 |
| Edry Property Limited | Real estate | 2021 |
| Edwards Coaches Limited | Administrative & support | 2000 |
| Edwards Hotels Limited | Hospitality | 2013 |
| Edwoods Golf Ltd | Wholesale & retail | 2021 |
| Effex Academy Ltd | Education | 2024 |
| EFL School Limited | Education | 2010 |
| Egnida Consulting Limited | Other services | 2017 |
| Egnida Energy Solutions Limited | Administrative & support | 2014 |
| Egnida Energy Trading Limited | Professional & scientific | 2014 |
| Egnida Group Limited | Other services | 2017 |
| Egnida Holdings Limited | Other services | 2018 |
| Egnida Innovation Limited | Other services | 2019 |
| EIN Glaswellt Farm Limited | Agriculture & fishing | 2014 |
| Eiphos Regeneration Ltd | Extraterritorial | 2025 |
| EJ Automotive Ltd | Wholesale & retail | 2021 |
| Elan Safety Ltd | Professional & scientific | 2024 |
| Electric HUT Llantrisant Ltd | Wholesale & retail | 2022 |
| Electrical Dynamic Systems Ltd | Construction | 2021 |
| Elements of Community CIC | Health & social care | 2023 |
| Elif Llanharry Ltd | Hospitality | 2025 |
| Elinor Keetch Ltd | Health & social care | 2009 |
| Elite Construction Solutions Limited | Construction | 2016 |
| Elite Fire & Security Ltd | Administrative & support | 2018 |
| Elite Optics Ltd | Wholesale & retail | 2018 |
| Elite Pro Environmental Ltd | Administrative & support | 2023 |
| Elite Property 1 Limited | Real estate | 2021 |
| Elite Vision Facilities Ltd | Administrative & support | 2026 |
| Elizabeth Jones Law Limited | Professional & scientific | 2013 |
| Elynor Backstedt Limited | Arts & recreation | 2019 |
| Embergoose Ltd | Professional & scientific | 2024 |
| Emerald Partnership Ltd | Real estate | 2023 |
| Emily Jayne Properties Ltd | Real estate | 2024 |
| Emily Rock Associates Ltd | Professional & scientific | 2024 |
| Emotive Communications Limited | Manufacturing | 2013 |
| Enchanted Bearwood Ltd | Arts & recreation | 2024 |
| Enchantedim Ltd | Wholesale & retail | 2025 |
| Energy Shed Limited | Administrative & support | 2022 |
| Engmec Solutions Ltd | Manufacturing | 2024 |
| Enoch Developments Limited | Construction | 2021 |
| Ensemble Cambrica Ltd | Arts & recreation | 2004 |
| Enterprise in Education Limited | Education | 1997 |
| EPS High Volt Limited | Manufacturing | 2014 |
| Equine & Commercial Trailer Repairs Ltd | Manufacturing | 2020 |
| Equip Coaching & Training Ltd | Other services | 2020 |
| Euro Performance Limited | Wholesale & retail | 2006 |
| Euroenviro Equipment Ltd | Manufacturing | 2004 |
| European Vocational Training Limited | Education | 1994 |
| Everyday Intelligence Limited | Information & communication | 2025 |
| Excel Home Design Ltd | Construction | 2006 |
| Excellent Landscapes Ltd | Construction | 2020 |
| Explore Accountancy Limited | Professional & scientific | 2025 |
| Explore Psychology Ltd | Education | 2024 |
| Exquis Ltd | Other services | 2019 |
| Extrascope Limited | Information & communication | 2009 |
| F&T Terrix Limited | Wholesale & retail | 2025 |
| F&T Terrix South Ltd | Wholesale & retail | 2025 |
| Facade Property Developments Ltd | Real estate | 2026 |
| Falco Building Solutions Ltd | Construction | 2024 |
| Falshaw Property Ltd | Real estate | 2026 |
| Fareva UK Limited | Professional & scientific | 2009 |
| Farihas Kitchen Limited | Hospitality | 2019 |
| Farmtrack Surveyors Ltd | Professional & scientific | 2022 |
| Fferm Duffryn Limited | Real estate | 2023 |
| Fillcare Limited | Manufacturing | 1981 |
| Finley Carpentry Limited | Construction | 2019 |
| Fire Room Food Ltd | Hospitality | 2024 |
| Firenze Future Ltd | Professional & scientific | 2023 |
| Firstchoice Fitness Limited | Arts & recreation | 2005 |
| Flag Alpha Diving Consultancy Ltd | Construction | 2024 |
| Floodlight (Holdings) Limited | Financial services | 2009 |
| Floodlighting and Electrical Services Limited | Construction | 1982 |
| Floor-De-Lee Limited | Construction | 2023 |
| Florimonte Holdings Ltd | Real estate | 2022 |
| Focuspool Ltd | Information & communication | 2025 |
| Fone FIX & Care Limited | Wholesale & retail | 2023 |
| Foot Prints Counselling and Education Ltd | Education | 2026 |
| Forest Wood Welding and Fabrication Ltd | Construction | 2025 |
| Fortune Woods Holding Ltd | Financial services | 2018 |
| Fortune Woods UK Limited | Manufacturing | 2011 |
| Fosters Family Cafe Ltd | Manufacturing | 2022 |
| FR Carriers Ltd | Transport & logistics | 2026 |
| Francass Specialist Ground and Haulage Works Limited | Construction | 2023 |
| Frank Nuttall Sports Management and Consulting Limited | Arts & recreation | 2001 |
| Franks by J&R Ltd | Hospitality | 2019 |
| Freaklizard Limited | Wholesale & retail | 2018 |
| Freeholders Group Limited | Extraterritorial | 2025 |
| Freewheel Leisure Limited | Wholesale & retail | 2005 |
| Fruitapeel (Juice) Ltd | Manufacturing | 2014 |