| 1 2 1 Hospitality Limited | Professional & scientific | 2011 |
| 1 Answer Insurance Services Ltd. | Financial services | 2001 |
| 1 Answer Network Limited | Professional & scientific | 2005 |
| 1 Call Safety Training Limited | Professional & scientific | 2022 |
| 1 Prop Co Ltd | Households | 2023 |
| 1111 SJS Ltd | Financial services | 2025 |
| 12 Grassington Road (Eastbourne) Management Limited | Households | 2003 |
| 17 the Avenue Eastbourne Limited | Households | 2007 |
| 1ST Education Limited | Information & communication | 2019 |
| 2 Chatsworth Gardens Management Company Limited | Households | 2007 |
| 24 Church Road TW Management Ltd | Real estate | 2021 |
| 24/7 Storage Solutions Ltd | Transport & logistics | 2018 |
| 247 247 Ltd | Transport & logistics | 2023 |
| 247 Transport Solutions Limited | Transport & logistics | 2009 |
| 24K Nails and Spa Limited | Other services | 2024 |
| 2S Recruitment Ltd | Administrative & support | 2018 |
| 2UK2 Ltd | Wholesale & retail | 2014 |
| 37 HS Steyning Ltd | Real estate | 2021 |
| 4-RIGHT.COM Ltd | Real estate | 2025 |
| 47 SQ MI Ltd | Wholesale & retail | 2025 |
| 67 Enys Road Management Company Limited | Households | 2007 |
| 69 Langney Road Ltd | Other services | 2024 |
| 80:20 Risk Management Limited | Professional & scientific | 2013 |
| 85/87 South Street Eastbourne Limited | Real estate | 2014 |
| 9 Grange Gardens Eastbourne Limited | Real estate | 1999 |
| 9 South Cliff Residents Company Limited | Households | 2010 |
| A Wilder Ltd | Real estate | 2018 |
| A&B Housing Limited | Real estate | 2024 |
| A&B Recruiting Limited | Administrative & support | 2025 |
| A. J. Walton Electrical Ltd | Construction | 2019 |
| Aampbis Limited | Information & communication | 2009 |
| ABL Flooring Ltd | Construction | 2022 |
| Above and Below Plumbing and Heating Ltd | Construction | 2025 |
| Accent Audio Ltd | Arts & recreation | 2019 |
| Accepted INS Ltd | Financial services | 2022 |
| Aced Contracting Ltd | Manufacturing | 2023 |
| Acorn Trees Ltd | Administrative & support | 2017 |
| ACT on IT | Education | 2009 |
| Activexplore Travel Ltd | Administrative & support | 2024 |
| AD Momentum Partners Limited | Professional & scientific | 2018 |
| Adad Co Ltd | Wholesale & retail | 2026 |
| Adam Hossany Ltd | Arts & recreation | 2024 |
| Advanced Seven Days Roof Care Ltd | Construction | 2026 |
| Affinity Fitness Coaching Ltd | Arts & recreation | 2022 |
| Affinityprop Limited | Real estate | 2023 |
| Agena Group Bidco Ltd | Other services | 2019 |
| Agena Group Ltd | Other services | 2019 |
| Agena Group Midco 1 Ltd | Other services | 2019 |
| Agena Group Midco 2 Ltd | Other services | 2019 |
| Agena Solutions Limited | Information & communication | 2008 |
| AGM Roofing Ltd | Construction | 2025 |
| AGS London Capital Ltd | Administrative & support | 2022 |
| Ahamraj Limited | Hospitality | 2019 |
| Airstream Communications Limited | Information & communication | 1988 |
| AJR Property Services Ltd | Real estate | 2013 |
| Ajrr Support Services Ltd | Administrative & support | 2018 |
| Akom Developments Limited | Real estate | 2017 |
| Akro Valve Limited | Manufacturing | 2002 |
| AL the Pieman Limited | Manufacturing | 2015 |
| AL4PHA6SIGMA Ltd | Information & communication | 2019 |
| Alam's Property Ltd | Real estate | 2025 |
| Albion Executive Limited | Transport & logistics | 2018 |
| Alchemy Accounting Ltd | Professional & scientific | 2009 |
| Alchemy Investors Ltd | Financial services | 2025 |
| Alchemy Telco Ltd | Administrative & support | 2016 |
| Alexandretta Ltd | Hospitality | 2019 |
| Alfriston Arts Limited | Wholesale & retail | 2024 |
| ALL Distinct Ltd | Professional & scientific | 2024 |
| ALL Sports Management Limited | Other services | 2012 |
| ALL Star Toys Limited | Wholesale & retail | 2016 |
| ALL Things Festival Limited | Information & communication | 2018 |
| ALL Things Fungi Ltd | Arts & recreation | 2023 |
| Allen Langrick Financial Ltd | Financial services | 2015 |
| Allthefours Limited | Transport & logistics | 2007 |
| Ally Security Ltd | Administrative & support | 2018 |
| Almleka Multimedia Group Ltd | Information & communication | 2024 |
| Alpha Corporate Training Services Limited | Professional & scientific | 2012 |
| Alpha Real Estate Investments Ltd | Financial services | 2020 |
| Alsimplyadhd Ltd | Other services | 2026 |
| Amathis Limited | Professional & scientific | 2006 |
| Amayi Capital Ltd | Financial services | 2021 |
| Amba Trade Ltd | Wholesale & retail | 2025 |
| Amberley (Eastbourne) Limited | Households | 2004 |
| Ambridge HR Ltd. | Administrative & support | 2017 |
| American Seasonal Home Ltd | Wholesale & retail | 2025 |
| Amicus Housing | Real estate | 2024 |
| Amplify Nexus Ltd | Professional & scientific | 2025 |
| AMS Industries Limited | Extraterritorial | 2013 |
| Anaya Case Management & Therapy Services Ltd | Health & social care | 2019 |
| Ancient Grains Ltd | Hospitality | 2020 |
| Ancient Sardinia Ltd | Hospitality | 2025 |
| Andre Boyard Perfumes Limited | Manufacturing | 2002 |
| Andrew Howard Eeles Limited | Education | 2020 |
| Angmering Park Gallops Ltd | Arts & recreation | 2020 |
| Animal Cancer Trust | Administrative & support | 2002 |
| Animal Magic Ltd | Other services | 2024 |
| Anthony Field Limited | Real estate | 2016 |
| Aperture Web Ltd | Information & communication | 2021 |
| Apex Guesting Limited | Administrative & support | 2026 |
| Apex Surveys (North) Ltd | Professional & scientific | 2006 |