| RDM Pitlochry Limited | Hospitality | 2023 |
| Reddscot Limited | Professional & scientific | 2006 |
| Redhill Retreat Ltd | Hospitality | 2024 |
| Reiss Hair Limited | Other services | 2018 |
| Replay Music Festival Ltd | Wholesale & retail | 2025 |
| RG Developments (Solar) Limited | Construction | 2023 |
| RJ Scott Enterprises Limited | Construction | 2025 |
| Robert Gray Interiors (RGI) Ltd | Wholesale & retail | 2023 |
| Robertson's Beauty and Nails Limited | Other services | 2024 |
| RP Properties Ltd | Real estate | 2025 |
| Ruments SAX Ltd | Real estate | 2003 |
| Russell Cheyne Photography Ltd | Information & communication | 2017 |
| S. Saint Electrical Ltd. | Construction | 2011 |
| Schiehallionlodge Ltd | Real estate | 2025 |
| Scot Active Ltd | Information & communication | 2025 |
| Scotia HSE Consultants Limited | Other services | 2025 |
| Scottish Archive Network Limited | Arts & recreation | 1998 |
| Scottish Canoe Association | Other services | 2000 |
| Sean Mclauchlan Electrical Ltd | Construction | 2026 |
| Shoot and Scoot Ltd | Manufacturing | 2022 |
| Signal Solar Energy Limited | Construction | 2021 |
| Signal Warranties Limited | Manufacturing | 2021 |
| SM Forestry Ltd | Agriculture & fishing | 2021 |
| Spa Uniq Limited | Other services | 2014 |
| Spotless Detailing Group Ltd | Wholesale & retail | 2023 |
| Springbank International Limited | Professional & scientific | 2004 |
| Sprout Perthshire Ltd | Wholesale & retail | 2023 |
| Sreenigameert Ltd | Administrative & support | 2024 |
| STA Trading Limited | Extraterritorial | 2016 |
| Stagfire Ecological Surveys Ltd. | Professional & scientific | 2011 |
| Stevie G Roofing Limited | Construction | 2016 |
| Stewart Electrical Services Ltd | Construction | 2025 |
| Sticklebrix Property Ltd | Real estate | 2017 |
| Strachan Projects Ltd | Administrative & support | 2019 |
| Strathstone Construction Ltd | Construction | 2024 |
| Stromberg Shipping Limited | Real estate | 2014 |
| Sylvia Laing Holdings Limited | Hospitality | 2025 |
| Taiga Nexus Ltd | Financial services | 2025 |
| Takemusu Aiki CIC | Education | 2025 |
| Takoma Tours & Events Ltd | Administrative & support | 2024 |
| Talbot Arms Milngavie Ltd | Hospitality | 2025 |
| Taste Talk Ltd | Hospitality | 2020 |
| Tatha Limited | Real estate | 2014 |
| Tatha Retreats Ltd | Hospitality | 2026 |
| TAY Ecology Ltd | Professional & scientific | 2015 |
| THE Birks Cinema Limited | Hospitality | 2012 |
| THE Birks Cinema Trust | Real estate | 2009 |
| THE Boat of Garten Hotel Limited | Hospitality | 2007 |
| THE Bridge of Pitlochry Ltd | Hospitality | 2022 |
| THE Cornerstone at Rannoch Limited | Hospitality | 2021 |
| THE Farmhouse Scotland Ltd | Hospitality | 2021 |
| THE Grandtully Hotel Ltd | Hospitality | 2026 |
| THE Griffin & Calliachar Community Endowment | Professional & scientific | 2021 |
| THE Hairy Coo’S Teecup Ltd | Hospitality | 2026 |
| THE Inn on the TAY Limited | Hospitality | 2009 |
| THE Inner Universe Scotland Ltd. | Administrative & support | 2025 |
| THE Luxury Fitness Group Ltd | Arts & recreation | 2013 |
| THE Northern Light Cruising Company Limited | Administrative & support | 2014 |
| THE Pine Trees Hotel Ltd. | Extraterritorial | 2011 |
| THE Plaice to BE Ltd. | Hospitality | 2014 |
| THE Quiet Edge (Scotland) Ltd | Administrative & support | 2026 |
| THE Rafting Company Limited | Arts & recreation | 2014 |
| THE Seaforth Inn Limited | Hospitality | 2014 |
| THE Tartan Awards Limited | Professional & scientific | 1998 |
| THE Wellwood House 1881 Ltd | Hospitality | 2023 |
| Thomson Garden Care Ltd | Administrative & support | 2026 |
| Thornhill Consulting Services Ltd | Professional & scientific | 2018 |
| Three Mccallum Limited | Administrative & support | 2020 |
| TLC Pitlochry Ltd | Administrative & support | 2019 |
| Tombane Farm Limited | Manufacturing | 2012 |
| Tomcur Properties Limited | Real estate | 2025 |
| Tradicle Limited | Administrative & support | 2023 |
| Trakminer Limited | Professional & scientific | 2003 |
| Tree Maintenance Services Ltd. | Administrative & support | 2017 |
| Treemagineers Ltd. | Professional & scientific | 2007 |
| Treepartner Ltd. | Agriculture & fishing | 2014 |
| Trescorp Limited | Real estate | 1988 |
| Tribu Wellbeing Limited | Health & social care | 2020 |
| Turas Hotels Limited | Financial services | 2007 |
| UK Canyoning Ltd | Education | 2021 |
| Urban Rural Design Ltd | Professional & scientific | 2016 |
| Velumbra Limited | Professional & scientific | 2026 |
| Verdant Healing Limited | Wholesale & retail | 2022 |
| W M Shorthouse Forestry Limited | Agriculture & fishing | 2014 |
| Walk Scotland Limited | Extraterritorial | 2022 |
| Wasted Degrees Holdings Ltd | Real estate | 2023 |
| Westlands Inn Ltd | Hospitality | 2021 |
| Westlands of Perthshire Ltd. | Hospitality | 2015 |
| Wilde Hydeout Ltd | Hospitality | 2026 |
| William Telford Construction Ltd | Construction | 2024 |
| Willpeople Limited | Professional & scientific | 2002 |
| Wilson's of Pitlochry Ltd | Wholesale & retail | 2016 |
| World Famous Foods Limited | Hospitality | 2025 |
| World of ILL Picture Company Ltd | Information & communication | 2026 |
| XL Training (Scotland) Limited | Education | 2003 |
| Your Scotland Guide Ltd | Administrative & support | 2026 |