| 4 MW (Scotland) Ltd | Manufacturing | 2022 |
| 53 Delivery Ltd | Wholesale & retail | 2020 |
| A.M.A. Hair Design Ltd. | Other services | 2005 |
| Acarsaid Hotel Ltd | Hospitality | 2013 |
| Activity Scotland Ltd | Professional & scientific | 2012 |
| After Hours Studios Ltd | Wholesale & retail | 2025 |
| AKG Homes Ltd. | Construction | 2013 |
| Alexander Macleod Limited | Professional & scientific | 2016 |
| Alltmor Retreat Ltd | Real estate | 2025 |
| ALR Technologies Limited | Construction | 2014 |
| AM Bùth Leighis Ltd | Wholesale & retail | 2024 |
| Andy Sweet Ltd | Professional & scientific | 2015 |
| Apbuilders Ltd | Construction | 2026 |
| APZ Creative Ltd | Information & communication | 2022 |
| Arbw Holdings Limited | Professional & scientific | 2010 |
| Artcliff Network Design Ltd | Information & communication | 2016 |
| Atholl Country Life Museum | Arts & recreation | 2004 |
| Atholl Hydro Limited | Energy | 2011 |
| Atholl Premier Limited | Wholesale & retail | 2026 |
| Atholl Properties Ltd | Real estate | 2022 |
| Atholl Prospect Limited | Professional & scientific | 1998 |
| Atholl Road Design Ltd | Information & communication | 2012 |
| Badvo Distillery Limited | Manufacturing | 2017 |
| Ballinluig Hospitality Limited | Hospitality | 2019 |
| Ballinluig Services Limited | Wholesale & retail | 2017 |
| Ballintaggart Events Limited | Hospitality | 2026 |
| Ballintaggart Farm Limited | Hospitality | 2026 |
| Ballintaggart Holdings Limited | Financial services | 2025 |
| Ballintaggart Retail Limited | Wholesale & retail | 2025 |
| Bealach Ltd | Real estate | 2025 |
| Beinn a Ghlo Ltd | Real estate | 2026 |
| BEN Y Vrackie Ltd | Real estate | 2025 |
| Bertie Browns Ltd | Wholesale & retail | 2025 |
| Bespoke Rafting Ltd | Arts & recreation | 2025 |
| BI BEO Ltd | Information & communication | 2011 |
| Bibs Digital Ltd | Professional & scientific | 2024 |
| Birkwood Scotland Ltd | Construction | 2026 |
| Blair Atholl and Struan Initiative | Other services | 2002 |
| Blair Atholl Village Hall | Arts & recreation | 2016 |
| Blair Castle Estate Limited | Agriculture & fishing | 1995 |
| Bmok Property Ltd | Real estate | 2025 |
| Bonskeid Music Ltd. | Information & communication | 1978 |
| Braes Enterprises Ltd | Hospitality | 2014 |
| Bridge of Tilt Company Limited | Administrative & support | 1964 |
| Brightside Planning Ltd | Other services | 2013 |
| Buttonboss Lodge Ltd | Hospitality | 2025 |
| Cable Solutions (Pitlochry) Ltd | Construction | 2025 |
| Cairngorm Hotel Limited | Hospitality | 1992 |
| Call of the Wild Distillers Ltd. | Wholesale & retail | 2019 |
| Casa Mons Ltd | Real estate | 2025 |
| Castle Hotel Strathpeffer Ltd | Hospitality | 2018 |
| Caterhaugh Limited | Information & communication | 2026 |
| Celtic Shopping Ltd | Wholesale & retail | 2015 |
| Clamarkal Limited | Administrative & support | 2013 |
| Climate Café CIC | Professional & scientific | 2021 |
| CMT Hospitality Ltd | Hospitality | 2024 |
| Cnoc an Ro-Aire Properties Limited | Real estate | 2001 |
| Codagen Ltd | Professional & scientific | 2025 |
| Coilavoulin and Ardgie Farm (1996) | | 1996 |
| Coorie in the Corner Limited | Extraterritorial | 2017 |
| Coryglas Limited | Agriculture & fishing | 2016 |
| Craig Ferns Inspection Services Limited | Professional & scientific | 2026 |
| Croftdouglas Vacation & Leisure Services Ltd | Hospitality | 2020 |
| Cuil an Daraich Guest House Limited | Hospitality | 2025 |
| Darach Albannach Limited | Hospitality | 2020 |
| Darkside Software Ltd. | Information & communication | 2013 |
| Derculich (Strathtay) Limited | Agriculture & fishing | 1997 |
| DMC Homes Ltd | Real estate | 2026 |
| Dowally Caravan Park Ltd | Hospitality | 2023 |
| Drummond Lawn Tennis Club Limited | Arts & recreation | 2024 |
| Dunalastair Estate Ltd | Agriculture & fishing | 2023 |
| Dunan Estate LLP | | 2025 |
| Dunkeld Mortgage House Ltd | Financial services | 2021 |
| Elizabeth Yule Transport Ltd | Transport & logistics | 2023 |
| Ensignia Print Solutions Limited | Manufacturing | 2009 |
| Erdol Properties Limited | Hospitality | 2022 |
| Escape Route Limited | Wholesale & retail | 2015 |
| Essential Scotland Limited | Professional & scientific | 1996 |
| ET Food Perthshire Limited | Hospitality | 2024 |
| Etojad Limited | Professional & scientific | 2018 |
| Eureka Stays Ltd | Hospitality | 2023 |
| Evolving Ventures Limited | Administrative & support | 2023 |
| Ewan Mcadam Joinery Ltd | Construction | 2021 |
| F Calder Contracting Ltd | Extraterritorial | 2024 |
| Fair Feast CIC | Manufacturing | 2024 |
| Feast Bites Ltd | Hospitality | 2025 |
| Fern Cottage Ltd | Hospitality | 2026 |
| Fisheries Innovation & Sustainability | Agriculture & fishing | 2014 |
| Fishstrip Limited | Extraterritorial | 2016 |
| Forster & Forster Solutions Ltd. | Transport & logistics | 2016 |
| Fyne Yacht Services Limited | Manufacturing | 2024 |
| G. Stiven Contract Management Ltd | Professional & scientific | 2022 |
| Garry House Associates Ltd | Education | 2017 |
| Ginger Organic Ltd | Real estate | 2026 |
| Glasgow Wines & Spirits Ltd | Wholesale & retail | 2021 |
| Glen Glack Limited | Hospitality | 2022 |
| Global Hotels Ltd | Hospitality | 2018 |
| Gomart Ltd | Wholesale & retail | 2025 |
| Goodwin Golias Ltd | Hospitality | 2025 |
| Grandtully Bridge Galleries Limited | Manufacturing | 2004 |