| Eastbourne Eco Action Network C.I.C. | Education | 2019 |
| Eastbourne Electrical LLP | | 2001 |
| Eastbourne Energy Ltd | Construction | 2020 |
| Eastbourne Property Shop Lettings Ltd | Real estate | 2018 |
| Eco Contractors & Sons Ltd | Construction | 2015 |
| Education Welfare Services Ltd | Health & social care | 2021 |
| Edwards BDP Limited | Construction | 2014 |
| Elden Accounting Services Ltd | Professional & scientific | 2018 |
| Electrical Solutions (S E) Limited | Construction | 2006 |
| Elegance Builders Ltd | Construction | 2017 |
| EVA & Gunna Dream Ltd | Wholesale & retail | 2025 |
| Eva's Beauty Ltd | Wholesale & retail | 2020 |
| Evasco Ltd | Manufacturing | 2025 |
| F P R Enterprises Limited | Households | 2014 |
| Fearfit Ltd | Wholesale & retail | 2020 |
| Fish Around Limited | Wholesale & retail | 2009 |
| Force 10 Ltd | Administrative & support | 2016 |
| Forge Place Management Company Limited | Households | 2019 |
| Forward Rentals Ltd | Real estate | 2024 |
| Fretley Property Management Ltd | Real estate | 2024 |
| Fretley Scientific Ltd | Professional & scientific | 2024 |
| Gateway Eastbourne Caterers Association (Geca) Ltd | Extraterritorial | 2019 |
| GB Concepts (INT) Limited | Construction | 2011 |
| Goldsmith Building and Maintenance Limited | Administrative & support | 2008 |
| GRB Vending Ltd | Information & communication | 2019 |
| Greenwich Energy Assessors Ltd | Professional & scientific | 2007 |
| GRT Civil Engineering Ltd | Administrative & support | 2011 |
| Hankham Lodge Ltd | Real estate | 2026 |
| Hannah Nails Pevensy Limited | Other services | 2024 |
| Hardwick Road (Eastbourne) Company Limited | Real estate | 1998 |
| Hawkfost Ltd | Construction | 2024 |
| HEALTH4LIFE C.I.C. | Arts & recreation | 2018 |
| Heatcell Limited | Professional & scientific | 2020 |
| Hedges Couriers Ltd | Transport & logistics | 2013 |
| Hellingly Rugby Football Club C.I.C. | Arts & recreation | 2017 |
| Heron Westham Limited | Hospitality | 2024 |
| Hide Studios Ltd | Professional & scientific | 2025 |
| Holistic Security Solutions Ltd | Administrative & support | 2016 |
| I & a Profit Ltd | Real estate | 2026 |
| Immersium Community CIC | Education | 2026 |
| Inspection, Service & Repair Ltd | Manufacturing | 2011 |
| Inspiration Decor Ltd | Other services | 2015 |
| Intelijet Carwash Limited | Wholesale & retail | 2023 |
| Invictanet Limited | Information & communication | 2001 |
| IQH Limited | Construction | 2009 |
| J L Financial Ltd | Professional & scientific | 2024 |
| J.Hayes Electrical Ltd | Construction | 2025 |
| James Charles Property Group Ltd | Real estate | 2025 |
| JB Hodges PAT Testing Ltd | Professional & scientific | 2025 |
| JDK Business Services Limited | Hospitality | 2018 |
| JDK Property Management Limited | Real estate | 2015 |
| JDM Technical Services Ltd | Construction | 2026 |
| JIB Software Ltd | Information & communication | 2022 |
| Jonathan LEE Construction Ltd | Construction | 2023 |
| Judith Massey Content Services Limited | Professional & scientific | 2018 |
| Julian Binks Plumbing & Heating Contractor Ltd. | Extraterritorial | 2018 |
| K D Plastering & Building Ltd | Construction | 2014 |
| Karlsen Associates Limited | Professional & scientific | 2011 |
| Kyanite Solutions Ltd | Wholesale & retail | 2026 |
| LANDLORDS4LANDLORDS Limited | Households | 2025 |
| Leaptwo Limited | Administrative & support | 1984 |
| Leexi Property Limited | Real estate | 2017 |
| Leeyin Property Limited | Real estate | 2017 |
| Life's a Ball Limited | Wholesale & retail | 2016 |
| Liquid AUTHENTIC8 Ltd | Professional & scientific | 2026 |
| Lively Hive Mead Co Ltd | Manufacturing | 2020 |
| Livid Contractors Ltd | Professional & scientific | 2019 |
| Loki T.V. Limited | Information & communication | 2001 |
| Ludovicus Ltd | Information & communication | 2022 |
| Lydd Kart Circuit Limited | Real estate | 2010 |
| Lydd Karting Limited | Arts & recreation | 2010 |
| Lyfelyne Limited | Health & social care | 2025 |
| M W Pyle Roofing Limited | Construction | 2002 |
| Made by ME Ltd | Construction | 2025 |
| Magnetic Flare Studios Ltd | Information & communication | 2025 |
| Magnificat Ltd | Professional & scientific | 2010 |
| Makeover Kitchens Limited | Construction | 2008 |
| Marley Furniture Ltd | Administrative & support | 2019 |
| Matt Carr Design Limited | Administrative & support | 2010 |
| MEN WHO Talk (Wealden) CIC | Health & social care | 2026 |
| Milhams (Eastbourne) Limited | Agriculture & fishing | 2011 |
| Mill Farm Clay Shooting Ground Ltd | Arts & recreation | 2025 |
| Mill Villas Residents Management Company Limited | Real estate | 2014 |
| MNG Electrical Contractors Ltd | Construction | 2025 |
| Monkey Puzzle Close Limited | Real estate | 2015 |
| MSK Martial Arts Limited | Arts & recreation | 2006 |
| MU Digital Assets Ltd | Financial services | 2026 |
| Multiplier Ltd | Professional & scientific | 2018 |
| N & M Cafe Ltd | Hospitality | 2019 |
| Nika Pet Food Ltd | Manufacturing | 2024 |
| NXT Boxing Ltd | Education | 2026 |
| O.E.Plus Ltd | Wholesale & retail | 2019 |
| OG Entertainment Limited | Hospitality | 2025 |
| OG Vending Limited | Hospitality | 2024 |
| Origo Boss Limited | Information & communication | 2007 |
| P & M Electrics Bexhill Ltd | Construction | 2019 |
| Paul S. Baker Ltd. | Professional & scientific | 2007 |
| Pawtopia Dog Rescue CIC | Other services | 2025 |
| PDB Build Ltd | Construction | 2019 |
| Peacocks Executive Care Ltd | Health & social care | 2024 |