| Scott & Valentine | | 1990 |
| Scottish Parachute Club | Transport & logistics | 1994 |
| Seggieden Garden | | 1983 |
| Sgps Trustee Limited | Financial services | 2024 |
| Shian Farms | | 1991 |
| Shirvan Consultants Limited | Real estate | 2002 |
| Spirit of Wood Limited | Wholesale & retail | 2003 |
| Spud Mulligan's Ltd. | Hospitality | 2004 |
| ST Fillans Store Limited | Wholesale & retail | 2012 |
| Stage-Coach International Services Limited | Professional & scientific | 1988 |
| Stagecoach BUS Holdings Limited | Financial services | 1997 |
| Stagecoach Group Limited | Transport & logistics | 1986 |
| Stagecoach Holdings Limited | Professional & scientific | 2000 |
| Stagecoach Pension Trustee Company Ltd. | Financial services | 2001 |
| Stagecoach Transport Holdings Limited | Professional & scientific | 1998 |
| Star Hotel Auchterarder Ltd. | Hospitality | 2002 |
| Steps of Cally Farm | | 1987 |
| Stewart Farms Limited | Agriculture & fishing | 1979 |
| Stirling Mushrooms | | 1997 |
| Strata Group Holdings (Scotland) Limited | Financial services | 1999 |
| Streetfield Property Company | Real estate | 1962 |
| Struan Motors Limited | Wholesale & retail | 1979 |
| Surjas Limited | Wholesale & retail | 2006 |
| Tarduff Farm | | 1990 |
| Tarewa Ltd | Construction | 2018 |
| Tasty FRY Limited | Hospitality | 2003 |
| TAY and Torridon Estates Limited | Professional & scientific | 1963 |
| Taymouth Courtyard Shop Ltd | Wholesale & retail | 2005 |
| Tayside Forestry Services Limited | Agriculture & fishing | 2003 |
| Temseaco Limited | Professional & scientific | 2005 |
| Tgfs (the Good Food Shop) Limited | Wholesale & retail | 2003 |
| THE Ardlarich and Killichonan Farming Company | | 1989 |
| THE Ballathie Fishings Timeshare Limited | Administrative & support | 2003 |
| THE Balwhyme Farming Partnership | | 1994 |
| THE Blackbank & Rait Farming Partnership (Partnership NO.2) | | 2001 |
| THE Cairn Partnership Limited | Real estate | 2005 |
| THE Compass Christian Centre Limited | Arts & recreation | 1968 |
| THE Dunblane Motor Company Limited | Wholesale & retail | 1938 |
| THE Fish & Chip Co Limited | Hospitality | 2002 |
| THE Green Welly Stop Limited | Wholesale & retail | 1998 |
| THE Guidelines International Network | Professional & scientific | 2003 |
| THE Highland Pony Society | Other services | 1994 |
| THE Hydropathic Hotel Pitlochry Limited | Hospitality | 2001 |
| THE Lair Farming Partnership | | 1989 |
| THE Motorhome Company (Perth) Limited | Wholesale & retail | 2015 |
| THE Netherholm Partnership | | 2001 |
| THE New Duncroisk Farm Partnership | | 2001 |
| THE New School, Butterstone | | 1999 |
| THE Sherriff Thomson Partnership | | 1997 |
| THE Shetland Pony Stud-Book Society | Agriculture & fishing | 1991 |
| THE Willy Wallace Hostel Limited | Hospitality | 1999 |
| Thomas Thomson (Blairgowrie) Limited | Agriculture & fishing | 1937 |
| Threave Home Hardware Limited | Wholesale & retail | 2000 |
| Tighnablair Farming Partnership | | 1988 |
| Tighnablair Farming Partnership | | 1997 |
| Timerek Limited | Administrative & support | 2000 |
| TLR Restorations Limited | Wholesale & retail | 2021 |
| Tombreck Farm | | 1993 |
| Tornado Wire Limited | Manufacturing | 1978 |
| Trade Solutions (Scotland) Limited | Manufacturing | 2000 |
| Transcotland Limited | Administrative & support | 1989 |
| Trean Farm Partnership | | 1985 |
| Tulloch Timber (Nairn) Limited | Manufacturing | 1993 |
| Typescape Design LLP | | 2022 |
| Unicorn Travel, Tours & Paddle Ltd. | Administrative & support | 2007 |
| Upper Muirhall Farming Company | | 1991 |
| Valentines of Crieff Limited | Wholesale & retail | 2001 |
| Viewbank Farms NO 2 | | 1997 |
| VL Tech Pro Ltd | Wholesale & retail | 2020 |
| W Douglas Contractors Limited | Administrative & support | 2007 |
| W Glen Limited | Agriculture & fishing | 2005 |
| W P Bruce (Peas) Limited | Agriculture & fishing | 2001 |
| W. M. Coupar Limited | Wholesale & retail | 1983 |
| W.P.Bruce Limited | Agriculture & fishing | 1961 |
| WB Dodds (Building Contractors) Limited | Construction | 2002 |
| Web Smart Media Limited | Information & communication | 2009 |
| Webxel.Com Limited | Information & communication | 2000 |
| Wellhill Farming Partnership | | 1988 |
| Wellhill Farming Partnership (NO. 2) | | 1992 |
| Wendy Homes Ltd | Real estate | 2022 |
| West Haugh Farm Limited | Extraterritorial | 1948 |
| Westburn Investments Ltd | Administrative & support | 2016 |
| Wester Campsie Farming 1996 | | 1997 |
| Wester Flawcraig Farms | | 1981 |
| Westertown Farm | | 1991 |
| Westertown of Blacklunans Farm | | 1988 |
| WI Smith | | 1984 |
| William KAY Ltd. | Manufacturing | 2003 |
| Woodhill and Ballintuim Farm | | 1988 |
| Woodland Management Services (Scotland) Ltd | Agriculture & fishing | 2021 |
| Write on Signs Limited | Manufacturing | 2005 |
| YOU Spa Ltd | Other services | 2022 |