| DH Heating Services Limited | Construction | 2004 |
| Dickens Inns Limited | Information & communication | 1997 |
| Dollerie Farm | | 1992 |
| Dolly's Beauty Limited | Other services | 2013 |
| Donald. K Mcgillivray | | 1991 |
| Drum of Kincairney Farmers | | 1992 |
| Drummond Properties Limited | Real estate | 2002 |
| Dunblane Kids Club | Health & social care | 1997 |
| Duncroisk Estates Limited | Hospitality | 2022 |
| Duncroisk Farming Partnership | | 1989 |
| Dunearn Property Management Ltd. | Construction | 2011 |
| Dunvorist Investments Limited | Other services | 2007 |
| Dylan Gibson Illustration Ltd | Arts & recreation | 2007 |
| Earth-Rising Ltd | Professional & scientific | 2020 |
| East Tulchan Farms | | 1994 |
| Easter Coshieville Farms | | 1977 |
| Easter Flawcraig Farms | | 1981 |
| Easter Mause Farms | | 1991 |
| Easter Tully Bannocher | | 1987 |
| Element Engineering UK Limited | Professional & scientific | 2004 |
| Element Wind Limited | Energy | 2007 |
| Embden Investments (6) Limited | Real estate | 2007 |
| Entice Creations Limited | Wholesale & retail | 1987 |
| Everyco Limited | Construction | 1997 |
| Explore Scotland Limited | Professional & scientific | 2004 |
| Fife Scottish Omnibuses Limited | Transport & logistics | 1948 |
| Filmedup Ltd | Information & communication | 2006 |
| Finchpine Limited | Real estate | 1994 |
| Findowie Farms | | 1999 |
| Findynate Farm Partnership | | 2000 |
| Fingask Events Limited | Hospitality | 2002 |
| Firm of Wester Ballindalloch and Kingarth Farms | | 1992 |
| Five Mile Limited | Health & social care | 1997 |
| Flower Power Lighting Limited | Wholesale & retail | 2011 |
| Forgings (UK) Ltd | Manufacturing | 2002 |
| Fossoway Fish Farming Limited Partnership | | 1987 |
| Fraser of Spittal | | 1986 |
| Freeland Services Limited | Construction | 1983 |
| Furnell Consultant Psychologist Limited | Other services | 2007 |
| G Carstairs Limited | Construction | 2007 |
| G.S. Brown Construction Limited | Construction | 1970 |
| Gardens Galore Limited | Administrative & support | 2004 |
| Gellybanks Farm | | 1992 |
| Geoffrey Bruce Limited | Agriculture & fishing | 2004 |
| Gerry Morgan Plumbing and Heating Limited | Construction | 2006 |
| Glascorrie Farming Co. | | 2005 |
| Glenbran Farmers | | 2000 |
| Glendelvine Game Farm Limited Partnership | | 1988 |
| Gleneagles Hotels Limited | Hospitality | 1986 |
| Gleneagles Resort Developments Limited | Administrative & support | 2001 |
| Glenearn Farms | | 1990 |
| Glenfarg Recycling Limited | Professional & scientific | 2003 |
| Gordon Motors (Crieff) Limited | Wholesale & retail | 2007 |
| Goukton Farm | | 2000 |
| Gourdie Farms | | 1994 |
| Gowrie and East Nether Benchil Farms | | 1992 |
| Gowrie Farm | | 1994 |
| Graham Wilson & Sons (Farms) Limited | Agriculture & fishing | 1980 |
| Grange Mechanical Services Ltd | Manufacturing | 2024 |
| Greenhill Farm Partnership | | 1997 |
| Grewar Farming (Careston) Ltd | Agriculture & fishing | 2021 |
| GRS North Ltd. | Real estate | 2018 |
| GUS Macdonald Electrical Ltd. | Construction | 2005 |
| H.M. Pattullo & Co. | Agriculture & fishing | 1955 |
| Halley Limited | Agriculture & fishing | 2000 |
| Haugh of Tulliemet Farms | | 1997 |
| Haygrove (Scotland) Partnership | | 1999 |
| Heartland Cares Collective Ltd | Health & social care | 2025 |
| Highland Country Buses Limited | Transport & logistics | 1995 |
| Highland Perthshire Communities Land Trust | Real estate | 2002 |
| Highland Perthshire Communities Partnership | Other services | 1997 |
| Highland Spring Limited | Manufacturing | 1979 |
| Hope Sixteen (NO.87) Limited | Manufacturing | 1986 |
| HS Tunes Ltd | Information & communication | 2024 |
| I & S Smith | | 1985 |
| Iain Pennycook Ltd. | Construction | 2003 |
| IAN Hope (Farms) Limited | Agriculture & fishing | 1961 |
| IAN Milne (Electrical) Limited | Construction | 1986 |
| Initiate Business Development Ltd. | Professional & scientific | 2004 |
| Inveredrie | | 1990 |
| Ivysister Ltd | Other services | 2018 |
| J & M Forbes Limited | Agriculture & fishing | 2006 |
| J. & a. Henderson, Limited | Agriculture & fishing | 1921 |
| J. Docherty & Sons Ltd. | Transport & logistics | 2007 |
| J.B. Murray Ltd. | Health & social care | 2003 |
| James Donald Grain Drying Services Limited | Administrative & support | 2002 |
| Jayne Birnie Limited | Hospitality | 2005 |
| Jessica Dickson Designs Ltd | Manufacturing | 2021 |
| JHM Nominees Limited | Professional & scientific | 1994 |
| JML Contracts Ltd. | Construction | 2005 |
| John Hill Butcher Limited | Wholesale & retail | 2001 |
| John Wilkie Papermill Services Limited | Other services | 2003 |
| JSR Services (Blairgowrie) Limited | Agriculture & fishing | 2005 |
| Justgrass Limited | Agriculture & fishing | 2000 |
| K-Mel Mushrooms | | 1999 |
| Kare Gardens Ltd. | Administrative & support | 2003 |
| KC Property Rentals Ltd. | Real estate | 1999 |
| Keithick Energy Ltd | Energy | 2012 |
| Keithick Farms Limited | Agriculture & fishing | 1963 |
| Keizen Limited | Information & communication | 1995 |