| Rural Studio Scot Ltd | Professional & scientific | 2024 |
| Russaig Holdings Limited | Professional & scientific | 2021 |
| Russell Repairs Limited | Wholesale & retail | 2016 |
| RW Construction Perth Ltd | Construction | 2023 |
| Ryan Ward Services Ltd | Mining & quarrying | 2016 |
| Rymco Ltd | Professional & scientific | 2018 |
| S & B 1 Ltd | Wholesale & retail | 2025 |
| S & G Baynes Contracting Ltd | Agriculture & fishing | 2019 |
| S Bellhouse Limited | Information & communication | 2021 |
| S Grampian Limited | Hospitality | 2026 |
| S Hutchison (Scone) Limited | Wholesale & retail | 2005 |
| S Kulik Brickwork Ltd | Construction | 2021 |
| S&S Joinery Perth Ltd | Construction | 2025 |
| S-Dec Limited | Manufacturing | 1995 |
| S. P. E. Cabling Limited | Other services | 1993 |
| S.J.B. Utilico Limited | Construction | 2021 |
| SA Letting Holdings Ltd | Other services | 2026 |
| SA Perth Limited | Wholesale & retail | 2020 |
| Safe-Assure LLP | | 2019 |
| Safebase Ltd | Real estate | 2023 |
| Safetrac Ltd | Other services | 2024 |
| Safita Ltd | Health & social care | 2019 |
| SAI Jasp Foods Ltd | Hospitality | 2021 |
| Saintamara Services Ltd | Administrative & support | 2026 |
| Saishakthi Ltd | Wholesale & retail | 2018 |
| Sale Developments Limited | Real estate | 1986 |
| Salect Senipon Limited | Real estate | 1996 |
| Salmon Fishing Surgery Limited | Administrative & support | 2014 |
| Saltire Drywall Contracts Ltd | Construction | 2019 |
| Saltire Screeding Limited | Construction | 2022 |
| Samara Jewellery Ltd | Wholesale & retail | 2020 |
| Samer Furniture Ltd | Wholesale & retail | 2020 |
| Sandgrouse Sporting Limited | Arts & recreation | 2024 |
| Sands Agri Ltd | Agriculture & fishing | 2018 |
| Sandwood Bay Limited | Professional & scientific | 2015 |
| Sandy Adams Engineering Ltd | Manufacturing | 2001 |
| Sandy Todd Ltd. | Extraterritorial | 2009 |
| Santone Enterprises Ltd | Wholesale & retail | 2019 |
| Saphire Services Ltd | Professional & scientific | 2021 |
| SAR Medical Ltd | Health & social care | 2023 |
| SAR Nominees Ltd | Agriculture & fishing | 2021 |
| Sara Cuisine Ltd | Hospitality | 2022 |
| Sarah Brown Charity Law Limited | Professional & scientific | 2024 |
| Sattar Perth Ltd | Hospitality | 2026 |
| Savoro Ltd | Hospitality | 2026 |
| Savvy Investment Partners Limited | Real estate | 2021 |
| SAW Retail Ltd | Wholesale & retail | 2026 |
| Sayer Contracting Ltd. | Construction | 2014 |
| SC Financial Consultancy Limited | Professional & scientific | 2021 |
| SC834572 Ltd | Hospitality | 2025 |
| Scappa Electrical Ltd | Construction | 2022 |
| SCG Security Group Ltd | Information & communication | 2022 |
| Scone Drainage Services Limited | Water & waste | 2018 |
| Scone Estates Limited | Real estate | 2021 |
| Scone Fish & Chips Shop Ltd | Hospitality | 2010 |
| Scone Hotel Ltd | Hospitality | 2023 |
| Scone Joiners Limited | Construction | 2017 |
| Scone Scaffold Solutions Ltd | Construction | 2026 |
| Scoria Estate Ltd | Real estate | 2024 |
| Scoria Restaurant Ltd | Hospitality | 2025 |
| Scot BB TEL Limited | Manufacturing | 2011 |
| Scot Properties Management Ltd | Households | 2025 |
| Scot Routes Limited | Administrative & support | 2009 |
| Scotbox Limited | Extraterritorial | 1999 |
| Scotfast Accounts Ltd | Professional & scientific | 2022 |
| Scotfast Haulage Ltd | Transport & logistics | 2022 |
| Scotfast Limited | Construction | 2016 |
| Scotforce Healthcare Ltd | Administrative & support | 2016 |
| Scotforce Recruitment Ltd | Administrative & support | 2014 |
| Scothunt Ltd | Arts & recreation | 2015 |
| Scotia Cabins Limited | Manufacturing | 2015 |
| Scotia Eco Electrical Ltd | Construction | 2015 |
| Scotland's Charity Air Ambulance | Health & social care | 2010 |
| Scotloo Limited | Extraterritorial | 1999 |
| Scotmx Events Ltd | Arts & recreation | 2019 |
| Scotscreed Limited | Manufacturing | 2016 |
| Scott Connections Limited | Wholesale & retail | 2017 |
| Scott Cramb Developments Limited | Construction | 2019 |
| Scott Inspect Ltd | Construction | 2026 |
| Scott Platforms Ltd | Construction | 2025 |
| Scott Torrance Consultancy Ltd | Professional & scientific | 2024 |
| Scottish and Southern Energy Power Distribution Limited | Energy | 2000 |
| Scottish Environment Link | Professional & scientific | 2003 |
| Scottish Gamekeepers Association | Other services | 2010 |
| Scottish Gamekeepers Association Charitable Trust | Other services | 2010 |
| Scottish Highers Online Ltd | Education | 2024 |
| Scottish Hydro Electric Power Distribution PLC | Energy | 2000 |
| Scottish Hydro Electric Transmission PLC | Energy | 2000 |
| Scottish Meat Training Limited | Education | 2019 |
| Scotus Tours Ltd | Administrative & support | 2010 |
| Scotwood Earthline Ltd | Professional & scientific | 2026 |
| Scpitcairngreen Ltd | Hospitality | 2016 |
| Sdbd Ltd | Health & social care | 2024 |
| SDL Scotland Limited | Transport & logistics | 2024 |
| SDR Electrical Limited | Construction | 2022 |
| SEA02 Limited | Other services | 2020 |
| Seablade CIC | Professional & scientific | 2024 |
| Seal Forestry Limited | Agriculture & fishing | 2002 |
| Sean Mansell Limited | Professional & scientific | 2022 |
| Seapark Books Ltd | Construction | 2014 |