| OIL Pollution Consultancy Limited | Professional & scientific | 2014 |
| OIL Services Group Limited | Manufacturing | 2008 |
| Oioi Studios Ltd | Other services | 2024 |
| Old Fargie Lodges Limited | Hospitality | 2025 |
| Old Orkney Ltd | Manufacturing | 2020 |
| Oldbaum Services Limited | Professional & scientific | 2005 |
| Olimpia Cleaners Ltd | Administrative & support | 2024 |
| Olive Tree Takeaway Perth Ltd | Hospitality | 2019 |
| Oliverburn Estate Limited | Real estate | 2024 |
| Omega MMA Ltd | Education | 2019 |
| Omeworks Limited | Construction | 2025 |
| ON TAP Water and Drainage Limited | Construction | 2014 |
| On-Point Bespoke Joinery Limited | Construction | 2023 |
| Once Upon a Tattoo Ltd | Arts & recreation | 2025 |
| Ondemand Products Limited | Wholesale & retail | 2021 |
| One Call Vehicle Services Ltd | Administrative & support | 2012 |
| One Performance Centre Limited | Arts & recreation | 2020 |
| Onemoredata Ltd | Information & communication | 2025 |
| OOT N Aboot Ltd | Administrative & support | 2025 |
| Open Door Detailing Ltd | Wholesale & retail | 2025 |
| Open User Systems Ltd | Information & communication | 2024 |
| Oracle Plumbing & Heating Engineers Limited | Extraterritorial | 2022 |
| Orbitline Delivery Ltd | Transport & logistics | 2026 |
| Orcahq Limited | Information & communication | 2026 |
| Organic Welltree Ltd | Manufacturing | 2016 |
| Origin Electrical Services (Scotland) Ltd | Construction | 2026 |
| Ormexa Ltd | Wholesale & retail | 2025 |
| Ormond Bar Ltd | Hospitality | 2025 |
| Osado Farm Management Limited | Agriculture & fishing | 2010 |
| Osprey Business Services Limited | Professional & scientific | 2025 |
| Osprey Tunnels Limited | Manufacturing | 2010 |
| Osscar Limited | Real estate | 2018 |
| Ossian Offshore Wind Farm Holdings Limited | Energy | 2022 |
| Ossian Offshore Wind Farm Limited | Energy | 2022 |
| OUR House (a Home for Young People) Limited | Health & social care | 2018 |
| OUT of This Galaxy Ltd | Wholesale & retail | 2024 |
| P & N Properties (Scotland) Limited | Real estate | 2024 |
| P M Will Consultancy Ltd | Information & communication | 2024 |
| P&G Properties (Perth) Ltd | Real estate | 2022 |
| P&R Contracting Ltd | Construction | 2024 |
| PA Cooperative Ltd | Professional & scientific | 2022 |
| Paco's Perth Limited | Hospitality | 2013 |
| Paddingtons Nursery LLP | | 2015 |
| Palocco Investments Ltd | Real estate | 2024 |
| Pan-Atlantic Entertainments Limited | Extraterritorial | 2001 |
| Papillon Consulting Ltd | Professional & scientific | 2025 |
| Paragon Media Group Ltd | Professional & scientific | 2026 |
| Paragon Protection Systems Ltd | Construction | 2015 |
| Paratha TE CHA Ltd | Hospitality | 2026 |
| Pariah Media Ltd | Information & communication | 2025 |
| Park Plumbing & Heating Limited | Construction | 2009 |
| Parkers Contracts Limited | Construction | 2013 |
| Partizan Gymnasium Ltd | Arts & recreation | 2025 |
| Pasco Limited | Hospitality | 2013 |
| PAT Wilson Farms LP | | 2003 |
| Paton Decorators Limited | Construction | 2016 |
| Patrick Gifford Ltd | Transport & logistics | 2025 |
| Patterson Engineering Limited | Professional & scientific | 2021 |
| Paws Park Perth Ltd | Arts & recreation | 2021 |
| Payyoor Properties Ltd | Real estate | 2021 |
| PBS Matters Limited | Professional & scientific | 2023 |
| Pbsuk Limited | Administrative & support | 2016 |
| PCF Geotechnical Ltd | Professional & scientific | 2016 |
| PCM East Ltd | Administrative & support | 2024 |
| Peak Analogue Ltd | Financial services | 2020 |
| Peak Rare Earths Limited | | 2023 |
| Peda Logistics Ltd | Transport & logistics | 2025 |
| Pegg 3D Express Ltd | Transport & logistics | 2025 |
| Pemberton QA Engineering Ltd | Information & communication | 2025 |
| Pemco Energy Supplies Limited | Professional & scientific | 2006 |
| Pentestly.Io Ltd | Information & communication | 2025 |
| People With a Mission Ministries | Other services | 2003 |
| Perfect Vendors Ltd | Wholesale & retail | 2024 |
| Performance Alpine Racing Limited | Education | 2023 |
| Perth & Kinross Adhd Support Group | Health & social care | 2011 |
| Perth & Kinross Association of Voluntary Service Limited | Health & social care | 1983 |
| Perth & Kinross Hub Limited | Other services | 2019 |
| Perth & Kinross Landscaping Ltd | Construction | 2026 |
| Perth and Kinross Countryside Trust | Other services | 1997 |
| Perth and Kinross Heritage Trust | Arts & recreation | 1988 |
| Perth and Kinross Society for the Blind | Health & social care | 2000 |
| Perth Asian Foods Ltd | Hospitality | 2025 |
| Perth Auto Centre Ltd | Wholesale & retail | 2014 |
| Perth Auto Recyclers Ltd. | Water & waste | 2004 |
| Perth Bearings Limited | Wholesale & retail | 2023 |
| Perth Bodyshop Limited | Wholesale & retail | 2020 |
| Perth Car Sales Ltd | Wholesale & retail | 2025 |
| Perth Cars Limited | Wholesale & retail | 2014 |
| Perth Castle Muay Thai Ltd | Arts & recreation | 2026 |
| Perth Catering Ltd | Hospitality | 2023 |
| Perth Ceramics Limited | Construction | 2018 |
| Perth Christian Fellowship Limited | Hospitality | 2017 |
| Perth Citizens Advice Bureau | Information & communication | 1994 |
| Perth City Apartments Limited | Real estate | 2018 |
| Perth City Centre Community Hub Limited | Other services | 2019 |
| Perth City Electric Limited | Manufacturing | 2022 |
| Perth City Properties Ltd | Real estate | 2022 |
| Perth Clean Machine Ltd | Administrative & support | 2024 |
| Perth Community Farm Ltd | Agriculture & fishing | 2018 |
| Perth Decorating Ltd | Construction | 2016 |