| ILP 19 Limited | Hospitality | 2019 |
| Imia Ventures Limited | Other services | 2026 |
| Imig Hand Car Wash Ltd | Administrative & support | 2026 |
| Impact Training Solutions Ltd. | Education | 2003 |
| IMS Auctions Limited | Wholesale & retail | 2021 |
| IMS Heat Pumps Limited | Construction | 1997 |
| IN the Air Ltd. | Arts & recreation | 2011 |
| INB Limited | Financial services | 2019 |
| Inchture Wraparound Childcare Ltd | Education | 2023 |
| Inchyra Designs Limited | Wholesale & retail | 2010 |
| Inchyra Events Ltd | Hospitality | 2013 |
| Independent Advocacy Perth & Kinross Limited | Health & social care | 2003 |
| Independent Financial Planning Services Limited | Financial services | 2023 |
| Indian Cook School Ltd | Hospitality | 2017 |
| Indie Spirits Limited | Wholesale & retail | 2008 |
| Influx Group Ltd | Administrative & support | 2017 |
| Inn Welly Limited | Hospitality | 2026 |
| Innes Associates Ltd | Administrative & support | 2009 |
| Innes Group Holdings Limited | Financial services | 2020 |
| Inpex Operations Australia PTY Ltd | | 2011 |
| Insert Buzzword Ltd | Information & communication | 2024 |
| Insett Ltd | Real estate | 2026 |
| Insight GEO Limited | Professional & scientific | 2010 |
| Insight Hospitality Limited | Professional & scientific | 2018 |
| Insightgeo Marine Limited | Construction | 2013 |
| Inspire Dance Studios Limited | Education | 2017 |
| Inspire G H Limited | Financial services | 2025 |
| Inspire IT Services Ltd | Administrative & support | 2008 |
| Inspire Properties (HRD) Limited | Real estate | 2025 |
| Instant Image (Perth) Limited | Professional & scientific | 2008 |
| Instant Vehicle Buyer Ltd. | Wholesale & retail | 2015 |
| Integral Leadership Development Ltd. | Other services | 2018 |
| Intelligent Wood Systems (IP) Limited | Professional & scientific | 2016 |
| Intelligent Wood Systems (North America) Limited | Wholesale & retail | 2015 |
| Intelligent Wood Systems Limited | Manufacturing | 2009 |
| Intellistat Ltd | Professional & scientific | 2009 |
| Interurban Developments Ltd. | Construction | 2012 |
| Interurban Lettings Ltd. | Real estate | 2018 |
| Inveraire Holdings Limited | Professional & scientific | 2012 |
| Inverallan Grounds Maintenance Ltd | Administrative & support | 2022 |
| Inverallan Landscapes Limited | Administrative & support | 2003 |
| Inverdunning Limited | Construction | 2003 |
| Inverness Precast Ltd | Manufacturing | 2011 |
| Invervar Hydro Development Limited | Energy | 2013 |
| Investor Capital Ltd | Financial services | 2026 |
| Invicta Access Limited | Administrative & support | 2025 |
| Iplantsman Ltd | Extraterritorial | 2023 |
| Iproperties Scotland Ltd | Real estate | 2022 |
| Ireland & Irvine Associates Ltd | Financial services | 2020 |
| Iris Shipping Ltd | Transport & logistics | 2025 |
| Irvine J Wann Joiner Limited | Construction | 2018 |
| ISJ Horticulture Limited | Wholesale & retail | 2023 |
| Islay House Hotel Limited | Hospitality | 2024 |
| Islay House Property Investment Limited | Real estate | 2024 |
| Ismaeel Books Limited | Wholesale & retail | 2023 |
| Istudio Technology Ltd | Information & communication | 2024 |
| Ivybankarran Limited | Hospitality | 2012 |
| Iwaki Management Services Limited | Professional & scientific | 2019 |
| IWB Surveys Limited | Administrative & support | 2016 |
| J & D Hair Electrical Contractors Ltd | Administrative & support | 2015 |
| J & E Smillie (Holdings) Ltd | Financial services | 2021 |
| J & E Smillie (Montrose) Limited | Agriculture & fishing | 1977 |
| J & S Mckenzie Group Limited | Financial services | 2018 |
| J a C Grierson Limited | Agriculture & fishing | 2006 |
| J Paralegal Services Ltd | Administrative & support | 2018 |
| J Q & SON Plumbers Ltd | Construction | 2013 |
| J R Evans Garden Services Ltd. | Other services | 2018 |
| J Sweeney and Sons Ltd | Construction | 2019 |
| J Van Vliet (Aberdeen) Limited | Wholesale & retail | 2007 |
| J Van Vliet (Inverness) Ltd | Wholesale & retail | 2008 |
| J Van Vliet (Zwetsloot) Limited | Wholesale & retail | 2005 |
| J&F Land Limited | Real estate | 2009 |
| J&S Contracts Perth Ltd | Construction | 2022 |
| J&S Maintenance Limited | Administrative & support | 2020 |
| J&S Mckenzie Property Limited | Real estate | 2020 |
| J. Van Vliet (Edinburgh) Limited | Wholesale & retail | 2006 |
| J.& E.Smillie (Growers) Limited | Agriculture & fishing | 1977 |
| J.K.B. (Investments) Limited | Real estate | 2000 |
| Jack Blooms Ltd | Wholesale & retail | 2025 |
| Jack Global Supply Ltd | Wholesale & retail | 2025 |
| Jackie Chan Joinery Ltd | Construction | 2024 |
| Jala Systems Engineering Limited | Information & communication | 2025 |
| James Barclay Flooring Ltd | Wholesale & retail | 2020 |
| James Carr & Sons (Sawmillers) Limited | Manufacturing | 1974 |
| James Mackenzie Joinery & Building Services Ltd | Construction | 2017 |
| James Mcewan & SON Limited | Other services | 2014 |
| James Moffat Construction Limited | Construction | 2007 |
| James Watson Ventilation Limited | Construction | 2007 |
| Jameson + Mackay (Trustees) Limited | Financial services | 2026 |
| Jameson + Mackay LLP | | 2020 |
| Janebrydon Consulting Limited | Professional & scientific | 2022 |
| Jaqol Consultancy Limited | Administrative & support | 2024 |
| Jarral Ltd | Wholesale & retail | 2025 |
| JAV Property Limited | Real estate | 2001 |
| JAZ Design and Marketing Consultants Ltd | Wholesale & retail | 2021 |
| JC Hollywood Ltd | Construction | 2021 |
| Jcdharty Ltd | Real estate | 2023 |
| JD Green Ltd. | Administrative & support | 2009 |
| JD Services (Scotland) Ltd | Construction | 2018 |
| JE Motorworks Ltd | Transport & logistics | 2020 |