| Eden Properties (Scotland) Ltd | Real estate | 2024 |
| Edgley Select Stays Ltd | Real estate | 2026 |
| Edinburgh Bathroom Installers Ltd. | Construction | 2016 |
| Edomidas Holdings Limited | Professional & scientific | 2015 |
| Education Centre - Centrum Edukacyjne Bajka CIC | Education | 2018 |
| Eduventurous Ltd | Education | 2023 |
| EEA Giovanazzi Ltd | Wholesale & retail | 2024 |
| Egas Properties Ltd | Real estate | 2020 |
| Egypt Express Ltd | Other services | 2015 |
| EH12 Developments Ltd | Hospitality | 2023 |
| EIGHTEEN10 Education Limited | Education | 2020 |
| EKO Stove Room Ltd | Wholesale & retail | 2023 |
| Eko-Installations Limited | Construction | 2013 |
| Elder & Paton (Angus) Limited | Wholesale & retail | 2020 |
| Elder & Paton (Holdings) Limited | Financial services | 2024 |
| Elder & Paton (Kirkcaldy) Limited | Wholesale & retail | 2020 |
| Elder and Paton Inverness Limited | Wholesale & retail | 2025 |
| Eleazar Joinery Ltd | Construction | 2024 |
| Electrical Project Solutions Scotland Limited | Construction | 2020 |
| Elegent Limited | Other services | 2024 |
| Elegent Properties Ltd | Real estate | 2025 |
| Elements of the Highlands Limited | Manufacturing | 2025 |
| Elite BM Services Limited | Wholesale & retail | 2021 |
| Elite Coffee (Distributors) Limited | Wholesale & retail | 2010 |
| Ellie GEE Civils Limited | Construction | 2016 |
| Elliott Allen Consulting Limited | Administrative & support | 2020 |
| Elmcliff Limited | Real estate | 1994 |
| Elrasheed D'Lite Limited | Hospitality | 2019 |
| Elsada Accounting Services Limited | Professional & scientific | 2014 |
| Elsdon 2 Ltd | Hospitality | 2022 |
| Elsdon Limited | Hospitality | 2019 |
| Elseedawys Limited | Health & social care | 2016 |
| Emily MAI Bridal Ltd | Wholesale & retail | 2021 |
| Emirami Group Ltd | Wholesale & retail | 2026 |
| Emma Newlands Aesthetics Academy Ltd | Education | 2026 |
| Empath Mental Health CIC | Health & social care | 2024 |
| Employment Law Perth Ltd | Professional & scientific | 2013 |
| Emrap Limited | Professional & scientific | 2012 |
| Encrypted Computing Limited | Information & communication | 1997 |
| Endeavour Asset Finance Ltd | Financial services | 2023 |
| Endeavour Recruitment Limited | Extraterritorial | 2016 |
| Energetic Performance Limited | Extraterritorial | 2010 |
| Energetic Project Management Limited | Professional & scientific | 2010 |
| Energy Electrician Ltd | Manufacturing | 2007 |
| Energy Savers Scotland Ltd | Professional & scientific | 2023 |
| Energy-Link Partnership Limited | Information & communication | 2000 |
| Engineering Services Provider Limited | Manufacturing | 2022 |
| Enriched Being Ltd | Agriculture & fishing | 2023 |
| Enrick Gridco Limited | Energy | 2016 |
| Enva Weee Recycling Scotland Limited | Water & waste | 2023 |
| Epic Alternative UK Limited | Financial services | 2017 |
| Epsco Limited | Administrative & support | 1992 |
| Equorum Ltd | Agriculture & fishing | 2019 |
| Erat Efisien (Lumut) Limited | Transport & logistics | 2022 |
| Ergtec Ltd | Manufacturing | 2025 |
| Ericht Roofing & Property Maintenance Limited | Construction | 2016 |
| Erin Yorkstons Psychology Ltd | Professional & scientific | 2025 |
| ERN Property Ltd | Real estate | 2024 |
| Errochty Financial Services Limited | Financial services | 2018 |
| Errol Garage LLP | | 2021 |
| Errol Park Hospitality Limited | Hospitality | 2024 |
| ESB (Coalburn 2) Ltd | Energy | 2025 |
| ESB (Coalburn) Ltd | Energy | 2024 |
| ESB (Data) Limited | Professional & scientific | 2026 |
| ESB (Harker) Ltd | Energy | 2024 |
| ESB (Scotland) Ltd | Energy | 2023 |
| Eskbank Trading Co. Limited | Wholesale & retail | 1981 |
| Eske Properties Limited | Construction | 2015 |
| Essence by EWA Ltd | Wholesale & retail | 2025 |
| Eternal W Ltd | Wholesale & retail | 2019 |
| Etive Gridco Ltd | Energy | 2014 |
| Euphoria Entity Limited | Hospitality | 2016 |
| Eureka Waste 2 Energy Ltd | Construction | 2017 |
| European Store Perth Ltd | Wholesale & retail | 2026 |
| European Utility Consultants Limited | Other services | 2000 |
| Eurosec Automation & Security Systems Limited | Information & communication | 2017 |
| Evanhen Ltd | Agriculture & fishing | 2024 |
| Even Keel Contracts Ltd | Administrative & support | 2013 |
| Even Keel Limited | Arts & recreation | 2007 |
| Evert Property Limited | Real estate | 1998 |
| Evolution E-Types Ltd | Wholesale & retail | 2019 |
| External Dispute Resolution Ltd | Professional & scientific | 2015 |
| Extra Factor Ltd | Professional & scientific | 2023 |
| EZ Van Sales Ltd | Wholesale & retail | 2025 |
| F&H Property Development Limited | Construction | 2017 |
| F.E.Macdonald GAS Services Ltd | Construction | 2020 |
| F25HOMES Ltd | Real estate | 2026 |
| Face DR Ltd | Health & social care | 2019 |
| Factory of Classics Ltd | Wholesale & retail | 2019 |
| Fair City Carpets Limited | Wholesale & retail | 2003 |
| Fair City Developments Limited | Construction | 2015 |
| Fair City First AID Limited | Education | 2025 |
| Fair City Precast Limited | Manufacturing | 1982 |
| Fair City Properties Ltd | Real estate | 2025 |
| Fair City Property Group Limited | Financial services | 2021 |
| Fair City Renewables Ltd | Construction | 2025 |
| Fair City Solar Ltd | Construction | 2026 |
| Fair City Ventilation Ltd | Construction | 2022 |
| Fairbank Decorators Limited | Construction | 1995 |
| Fairbank Motors (Perth) Ltd | Wholesale & retail | 2017 |