| A4ACCOUNTS ( Yorkshire ) Limited | Professional & scientific | 2010 |
| Asperitas Limited | Information & communication | 2017 |
| Atomic Roman Limited | Transport & logistics | 2020 |
| Awaken IT Ltd | Information & communication | 2009 |
| AWP Consulting Services Limited | Professional & scientific | 2026 |
| Blacksmith's Arms (Penistone) Limited | Hospitality | 2025 |
| Blue Box IT Limited | Information & communication | 2002 |
| Borsuk Consulting Limited | Professional & scientific | 2026 |
| Buckle Barton Bookkeeping and Accountancy Services Ltd | Professional & scientific | 2018 |
| Build 4 U Demolition Ltd | Construction | 2026 |
| Build 4 U Interiors Ltd | Manufacturing | 2026 |
| Bulk Group Limited | Extraterritorial | 2022 |
| Bulk Metals Limited | Water & waste | 2009 |
| Cliffe Consultancy & Safety Services Ltd | Administrative & support | 2012 |
| Discrete Solutions Limited | Construction | 2015 |
| DW Software Engineering Ltd | Information & communication | 2022 |
| Goldthorpe's(Cornmillers)Limited | Manufacturing | 1960 |
| Grandpa Thomas & Sons Limited | Wholesale & retail | 2012 |
| Green Gate Capital Investment Ltd | Real estate | 2026 |
| High Marnham a Ltd | Real estate | 2025 |
| Hymarnham Power (Nottingham) Limited | Manufacturing | 2024 |
| IAN Bailey Carpets Limited | Wholesale & retail | 2003 |
| James H. Wood Printers Limited | Manufacturing | 1974 |
| Lavender International NDT Consultancy Services Limited | Professional & scientific | 1984 |
| M W Associates Limited | Professional & scientific | 1972 |
| MAW Utilities Limited | Energy | 2014 |
| Mclong Holdings International Limited | Financial services | 2025 |
| Mclong Holdings UK Limited | Financial services | 2025 |
| N.R.Paper Recycling (U.K) Ltd | Wholesale & retail | 2024 |
| NRP Consultants Limited | Information & communication | 2007 |
| Penfm Media C.I.C. | Information & communication | 2018 |
| Penistone Engineering Limited | Professional & scientific | 2018 |
| Penistone Interiors Limited | Manufacturing | 2021 |
| Penistone Line Partnership | Other services | 2001 |
| Pennine IT Consultants Ltd | Information & communication | 2023 |
| Phays Ltd | Professional & scientific | 2010 |
| Rainbows Inc. Ltd. | Health & social care | 2019 |
| Rascalz Childcare Limited | Health & social care | 2017 |
| Rascalz Soft Play Limited | Health & social care | 2017 |
| Robinsonsfm Limited | Administrative & support | 2018 |
| Rockleon Distribution UK Limited | Wholesale & retail | 2016 |
| Rockleon Property UK Limited | Construction | 2018 |
| Ross Whitmore Personal Training Ltd | Education | 2024 |
| Rostra Surgical Ltd | Health & social care | 2020 |
| Senlim Limited | Other services | 2015 |
| Smart Accounting Solutions (NW) Limited | Professional & scientific | 2026 |
| Springvale Commercial Letting Limited | Real estate | 2013 |
| SR Early Years Limited | Education | 2013 |
| ST3D Ltd | Professional & scientific | 2019 |
| Stanley Wire Limited | Manufacturing | 2020 |
| THE Buxton Cheese Company Ltd | Extraterritorial | 2009 |
| THE Great Cheese Company Ltd | Extraterritorial | 2009 |
| Train to Code Ltd | Information & communication | 2025 |
| Wizz Pro Ltd | Wholesale & retail | 2022 |
| Woodcock Rural Surveyors Ltd | Administrative & support | 2024 |
| Wordsworth Court (Penistone) Limited | Administrative & support | 1988 |