| Silicone Sealant Solutions Ltd | Construction | 2025 |
| Silva Digital Ltd | Information & communication | 2025 |
| SIR Matt Busby Ltd | Hospitality | 2019 |
| Smartbrandmind Ltd | Wholesale & retail | 2024 |
| Smooth Plaster Ltd | Construction | 2024 |
| Soave HS Ltd | Construction | 2026 |
| Solid Form Solutions Limited | Professional & scientific | 2008 |
| Soulfully Encompassed Ltd | Health & social care | 2025 |
| South Edinburgh Paving Ltd | Administrative & support | 2023 |
| Space by K8 Ltd | Other services | 2026 |
| Sportsmasters (UK) Limited | Administrative & support | 2006 |
| Springfield (Leadburn) LLP | | 2010 |
| SPT Group Ltd | Wholesale & retail | 2025 |
| Stair Assist Ltd | Professional & scientific | 2013 |
| Stakkrun Tech Consulting Ltd | Information & communication | 2026 |
| Sterry-Walters Partnership Ltd | Professional & scientific | 2011 |
| Stirling Salvage Limited | Transport & logistics | 2007 |
| Storezone Limited | Transport & logistics | 2000 |
| Structegrity Ltd | Professional & scientific | 2011 |
| Sultan Lounge Penicuik Ltd | Hospitality | 2024 |
| Supreme Property Rentals Limited | Real estate | 2020 |
| SVH Heating and Plumbing Limited | Construction | 2025 |
| SWW Consulting Ltd | Information & communication | 2021 |
| Systemas Consultancy Ltd | Information & communication | 2016 |
| T Hardwick Editorial Services Ltd | Other services | 2024 |
| Telford Taxis Ltd. | Professional & scientific | 2005 |
| THE Byre by the Sea Ltd | Real estate | 2025 |
| THE Door Centre Limited | Manufacturing | 1988 |
| THE Drip Edinburgh Limited | Hospitality | 2019 |
| THE Fire and Security Maintenance Company Ltd | Construction | 2011 |
| THE Fitness Education Academy Limited | Education | 2009 |
| THE Glencorse Association | Other services | 2007 |
| THE Legion Penicuik Ltd | Hospitality | 2026 |
| THE Mind Changers Limited | Information & communication | 2024 |
| THE Moredun Foundation | Agriculture & fishing | 1994 |
| THE North ESK Reservoir Company Limited | Real estate | 2013 |
| THE Penicuik & District Young Men's Christian Association and Young Women's Christian Association | Health & social care | 2009 |
| THE Penicuik Estate Partnership LLP | | 2020 |
| THE Pennycook Chippy Limited | Hospitality | 2025 |
| THE Performance Philosophy Limited | Education | 2023 |
| THE Podiatrist Clinics Limited | Health & social care | 2025 |
| THE SAM Wilson Ltd | Other services | 2025 |
| THE Secret Hideaway Glencorse Ltd | Hospitality | 2021 |
| THE Stackyard Park Secure Dog Parks Limited | Arts & recreation | 2024 |
| THE Sweeper Group Ltd | Administrative & support | 2024 |
| THE Trusted Companion Midlothian Ltd | Public administration | 2025 |
| TM Logan Consultancy Ltd | Professional & scientific | 2020 |
| Tmproperty Ventures Ltd | Real estate | 2024 |
| TNC Electrics Ltd | Construction | 2018 |
| Torridon Homes Ltd | Construction | 2025 |
| Tower Bites Ltd | Hospitality | 2025 |
| Truspin Limited | Manufacturing | 2018 |
| TS Joinery (Scotland) Ltd | Construction | 2025 |
| TWENTY20 Technology Ltd | Information & communication | 2013 |
| UK Travel Management Ltd | Administrative & support | 2024 |
| Ukua Group Ltd | Construction | 2019 |
| Uniq Web Management Ltd | Professional & scientific | 2023 |
| Upmarket (John ST) Ltd | Wholesale & retail | 2026 |
| Vaka Mobile Foot Specialist Ltd | Health & social care | 2023 |
| Valley Landscaping Ltd | Administrative & support | 2014 |
| Valleyfield Arts Ltd | Arts & recreation | 2025 |
| Valleyfield Conservatoire Ltd | Information & communication | 2025 |
| Vermilion Public Relations Ltd | Professional & scientific | 2024 |
| Vertex Steel Fabrication Ltd | Manufacturing | 2019 |
| Vertical Ropes Limited | Construction | 2025 |
| Vinatech Limited | Information & communication | 2026 |
| Virtuous Care Resources Limited | Health & social care | 2019 |
| Vitur Limited | Real estate | 2022 |
| Voltish Construction and Utilities Ltd | Construction | 2025 |
| WDN Commercial Ltd | Real estate | 2022 |
| Wells Wynd Developments Ltd | Construction | 2025 |
| Wendy Mitchell Ltd | Administrative & support | 2023 |
| WJS Builders Limited | Construction | 2021 |
| Wormvax Limited | Extraterritorial | 2009 |
| Wrightway Management Limited | Real estate | 2014 |
| WSC Paving Ltd | Construction | 2025 |
| Yagna Consulting Ltd | Information & communication | 2019 |
| Yjmj Spice Ltd | Hospitality | 2025 |
| YL Dream Strategy Ltd | Professional & scientific | 2025 |
| Yokohama Ltd | Hospitality | 2024 |
| Zebra Leisure Limited | Hospitality | 2019 |