| D & H Stoddart Limited | Agriculture & fishing | 2005 |
| DAA Site Management Limited | Construction | 2023 |
| Dakm.Wojciechowski. Ltd | Transport & logistics | 2025 |
| Dalex Systems Ltd | Construction | 2007 |
| DANCE4PASSION Limited | Arts & recreation | 2016 |
| Darren Main Electrical Services Ltd | Construction | 2016 |
| David Ross Catering Limited | Hospitality | 2024 |
| David W Reid Haulage Ltd | Transport & logistics | 2025 |
| Davidsons Taxis Ltd. | Transport & logistics | 2000 |
| Dempco Ltd | Construction | 2019 |
| Designshop (Edinburgh) Ltd. | Wholesale & retail | 2001 |
| Dexters Developments Ltd | Real estate | 2025 |
| Dial a Ground Limited | Construction | 2025 |
| Dillon Moore Property Ltd | Real estate | 2026 |
| Dino's Electrical Limited | Construction | 2017 |
| Draftarc Ltd | Information & communication | 2026 |
| Dragonfly Data Science Ltd | Administrative & support | 2026 |
| DRP Developments Ltd | Extraterritorial | 2015 |
| E3D Limited | Other services | 2000 |
| EA1 Agile Ltd | Professional & scientific | 2024 |
| Eastbar Limited | Transport & logistics | 2000 |
| Eastfield News Agent Ltd | Wholesale & retail | 2017 |
| Eastside Rapids Ltd | Education | 2019 |
| ECK Security Limited | Administrative & support | 2019 |
| Ecurie Ecosse Promotions Ltd | Administrative & support | 2012 |
| ED Elite Limited | Other services | 2024 |
| Edenmill Limited | Professional & scientific | 1999 |
| EDI Electrical Services Ltd | Construction | 2026 |
| Edinbangla Limited | Real estate | 2024 |
| Edinburgh Bio-Parks Limited | Extraterritorial | 1996 |
| Edinburgh Genetics Group Limited | Administrative & support | 2020 |
| Edinburgh Genetics Limited | Manufacturing | 2019 |
| Edinburgh Genetics Services Limited | Manufacturing | 2020 |
| Edinburgh Laser Optics Ltd. | Professional & scientific | 2009 |
| Edinburgh Luxury Stays Limited | Real estate | 2021 |
| Edinburgh Micro Hire Ltd | Administrative & support | 2025 |
| Edinburgh Pharmaceutical Processes Limited | Professional & scientific | 1992 |
| Edinburgh Repair Specialists Ltd | Administrative & support | 2021 |
| EH26 Cafe Ltd | Hospitality | 2025 |
| Elite Developers Scotland Ltd | Construction | 2025 |
| Endodx Limited | Professional & scientific | 2026 |
| Energy Transition Solutions Limited | Professional & scientific | 2025 |
| ESK Gardening and Maintenance Ltd | Administrative & support | 2026 |
| ESR Property (Scotland) Ltd | Real estate | 2023 |
| EVALU8 Science Limited | Extraterritorial | 2009 |
| Evidential Systems Ltd | Information & communication | 2009 |
| Explosoft UK Ltd | Information & communication | 2022 |
| Fallhills Consulting Limited | Professional & scientific | 2019 |
| Fine Food Holdings Limited | Wholesale & retail | 2015 |
| Flowers by Lindsey Limited | Wholesale & retail | 2016 |
| Flymax Limited | Wholesale & retail | 2025 |
| FM Health & Fitness Ltd | Health & social care | 2016 |
| Food Lodge Limited | Hospitality | 2026 |
| Forsyth Metal Service Limited | Manufacturing | 1999 |
| Fortis Property Holdings Ltd | Real estate | 2021 |
| Fortune Well Ltd | Hospitality | 2026 |
| Fourward Architecture & Masterplanning Ltd | Professional & scientific | 2015 |
| Fourward Design Ltd | Professional & scientific | 2015 |
| Fraser Russell Electrical Ltd | Construction | 2024 |
| Front and Centre Bars Limited | Hospitality | 2024 |
| Frontier Forestry Ltd. | Agriculture & fishing | 1998 |
| FSI Trading Limited | Wholesale & retail | 2020 |
| Full Circle Analytics Ltd | Information & communication | 2020 |
| Future Technos & Construction Ltd | Information & communication | 2018 |
| G Stewart Joinery Limited | Construction | 2023 |
| G T Contracting Limited | Construction | 2015 |
| GB Energy (Leadburn) Limited | Energy | 2016 |
| Geeraf Ltd | Construction | 2018 |
| GEM Farming Ltd | Agriculture & fishing | 2025 |
| GENER8 Science Limited | Extraterritorial | 2009 |
| Gifts 4 Everyone Ltd | Wholesale & retail | 2017 |
| Gifts by Marisa Ltd | Wholesale & retail | 2019 |
| Gillespie & Ross Ltd | Other services | 2015 |
| Gingersnap Design Limited | Professional & scientific | 2011 |
| Giovanni Bistro & Family Ltd | Hospitality | 2019 |
| Golden Bell Chinese Ltd | Hospitality | 2020 |
| Greenfield Stitchery Limited | Manufacturing | 2019 |
| Greenstone Energy Solutions Limited | Construction | 2009 |
| Ground Neutral Ltd | Financial services | 2021 |
| Grown Local Scotland Ltd | Agriculture & fishing | 2019 |
| Gupc Ltd | Water & waste | 2024 |
| GWS Wind Ltd | Construction | 2024 |
| Gymtastic (Fife) Limited | Arts & recreation | 2025 |
| Gymtastic (West Lothian) Limited | Arts & recreation | 2024 |
| Gymtastic Bathgate Limited | Arts & recreation | 2017 |
| Gymtastic Penicuik Ltd | Arts & recreation | 2009 |
| Haig GAS Services Ltd | Professional & scientific | 2022 |
| Halley Outdoors Ltd | Administrative & support | 2026 |
| Handykrystof Ltd | Construction | 2011 |
| Hanks Consulting Limited | Professional & scientific | 2019 |
| Happy Business Global Ltd | Other services | 2015 |
| Hayward & Stott Limited | Manufacturing | 2011 |
| Headcases Ltd | Other services | 2015 |
| Henderson Private Hire Limited | Transport & logistics | 2026 |
| Henry Torrance Consulting (2025) Ltd | Professional & scientific | 2025 |
| Hodgeada Ltd | Construction | 2025 |
| Home & Respite Care UK Limited | Health & social care | 2024 |
| Hopefield Partnerships Limited | Real estate | 2011 |
| Hutchison (DDS) Legal Services Ltd | Administrative & support | 2023 |
| I AM Abundant Ltd | Wholesale & retail | 2025 |