| East Sussex Developments Ltd | Construction | 2024 |
| Easyretail Ltd | Wholesale & retail | 2024 |
| Echelon Industries Ltd | Administrative & support | 2025 |
| Ecopool Finishes Ltd | Construction | 2020 |
| EL Cobra Limited | Public administration | 2021 |
| Elizabeth Developments Ltd | Construction | 2025 |
| Ellis Hair Limited | Other services | 2025 |
| Elltri Consulting Ltd | Information & communication | 2024 |
| Elmkm Ltd | Professional & scientific | 2025 |
| Embers Restaurant Brighton Ltd | Hospitality | 2022 |
| Emico Complete Building Solutions Ltd | Construction | 2022 |
| Emporio David Enterprises Limited | Construction | 1987 |
| Encore London Ltd | Construction | 2018 |
| Energy Construction Ltd | Construction | 2026 |
| Enhanceddw Limited | Wholesale & retail | 2025 |
| Envisage Property Investments Ltd | Real estate | 2018 |
| ES Fastfood Ltd | Hospitality | 2024 |
| Esdfa Ltd | Construction | 2026 |
| ESM259 Ltd | Professional & scientific | 2020 |
| Esprit Surveying Ltd | Professional & scientific | 2010 |
| Ethius Limited | Transport & logistics | 2018 |
| Eunysiza Healthcare Service Ltd | Health & social care | 2014 |
| Everyday Athletes Ltd | Education | 2024 |
| EWS Enterprise Ltd | Hospitality | 2022 |
| Experience IT NOW Travel Limited | Administrative & support | 2017 |
| Express Roofing BTN Ltd | Construction | 2026 |
| EXR Commerce Ltd | Wholesale & retail | 2025 |
| Eziflate Ltd | Manufacturing | 2023 |
| Fairy Dog Mother’S Dog Walking & Pet Services Limited | Other services | 2021 |
| Family Origins Design Ltd | Manufacturing | 2024 |
| Fantastic Tech Ltd | Information & communication | 2023 |
| Fantazia Trading Ltd | Construction | 2018 |
| Farrington Property Developments Limited | Real estate | 1984 |
| FCM Enterprise Ltd | Real estate | 2026 |
| Fearless Academy Ltd | Other services | 2025 |
| Feast Kitchen Limited | Hospitality | 2024 |
| Fenesse Ltd | Wholesale & retail | 2021 |
| FF Accounts Limited | Professional & scientific | 2018 |
| Film Sussex CIC | Information & communication | 2024 |
| Firehorse AI Ltd | Information & communication | 2026 |
| Fischer-Jones Ltd | Professional & scientific | 2015 |
| Fitzmount Ltd | Professional & scientific | 2022 |
| Format Electronics & Design Ltd | Professional & scientific | 2026 |
| Fortell Building Services Limited | Construction | 1979 |
| FOY Consulting (UK) Limited | Professional & scientific | 2009 |
| Fresh Point Retail Ltd | Wholesale & retail | 2026 |
| Friends of Beachy Head Countryside Centre Limited | Wholesale & retail | 2006 |
| FUN Team Ltd | Information & communication | 2011 |
| Furner Drylining Limited | Construction | 2025 |
| Future Guarding Solutions Ltd | Administrative & support | 2017 |
| Future Systems Property Services Ltd | Construction | 2016 |
| Futuretobe Limited | Professional & scientific | 2001 |
| Fxai Limited | Professional & scientific | 2019 |
| G & M Brand Publications Limited | Manufacturing | 1973 |
| G&N Garage Doors Ltd | Construction | 2025 |
| Gabriel (2017) Ltd | Manufacturing | 2017 |
| Garalis Partners Ltd | Administrative & support | 2025 |
| Geezers & Geezettes Ltd | Health & social care | 2026 |
| Gibson Search Ltd | Information & communication | 2017 |
| Giroux AI Ltd | Information & communication | 2024 |
| Giroux Estates Ltd | Real estate | 2020 |
| Giroux Limited | Information & communication | 2007 |
| Glass Centre (South East) Ltd | Construction | 2008 |
| Glide Training Ltd | Information & communication | 2011 |
| Global Hair Limited | Other services | 2008 |
| Global Reporting Company Limited | Financial services | 2013 |
| Gloo Entertainment Ltd | Information & communication | 2022 |
| Glovehand Limited | Households | 1996 |
| GNK Group Limited | Wholesale & retail | 2025 |
| Goatglobalco Limited | Wholesale & retail | 2025 |
| Goodfellas Pizza Van Limited | Hospitality | 2025 |
| Govr Ltd | Arts & recreation | 2017 |
| GPS Funerals Ltd | Other services | 2024 |
| Gregory GAS Heating & Plumbing Ltd | Construction | 2020 |
| Grover Property Developments Ltd | Construction | 2025 |
| GWH Residential Lettings Ltd | Real estate | 2026 |
| Habitation Holdings Ltd | Wholesale & retail | 2022 |
| Habitation Superstore Ltd | Wholesale & retail | 2024 |
| Hapticos Ltd | Real estate | 2021 |
| Hardknoxflooring Limited | Construction | 2017 |
| Harmonious Interiors Ltd | Other services | 2025 |
| Harpers Event Management Ltd | Administrative & support | 2013 |
| Haru Lewes Ltd | Hospitality | 2026 |
| Harvest Kitchen Ltd | Hospitality | 2026 |
| Harveys Property Management Limited | Professional & scientific | 2005 |
| Haven & Horizon Developments Ltd | Construction | 2026 |
| Havens Community Hub Community Interest Company | Administrative & support | 2018 |
| Haworth Property Investment's Ltd | Real estate | 2026 |
| Headway Roofing Ltd | Construction | 2014 |
| Helix Homes Building and Development Limited | Construction | 2020 |
| Helix Homes Construction Limited | Extraterritorial | 2015 |
| Helix Homes Developments Limited | Construction | 2014 |
| Helix Homes Properties Ltd | Extraterritorial | 2018 |
| Hellis&Bstays Ltd | Real estate | 2025 |
| Help UK CC Ltd | Extraterritorial | 2026 |
| HEN House Private Ltd | Hospitality | 2026 |
| HERO369 Ltd | Hospitality | 2026 |
| Highviews Building Services Ltd | Construction | 2025 |
| Hilton Investments Ltd | Financial services | 2025 |
| Hine Roofing Ltd | Construction | 2014 |