| Doric Spirit Limited | Manufacturing | 2025 |
| Double Crown Properties Ltd | Real estate | 2021 |
| Douglas & Smyth LLP | | 2004 |
| Down to Earth Gardening Services Ltd | Administrative & support | 2023 |
| Dozy Foods Ltd | Hospitality | 2015 |
| DP Office Marketing Limited | Extraterritorial | 2014 |
| DP Services Scotland Ltd | Administrative & support | 2026 |
| DR Garden Design Ltd | Professional & scientific | 2024 |
| DR Stoves and Landscapes Ltd | Construction | 2024 |
| Draestheticsmuse Limited | Other services | 2025 |
| Dragon Aquatics Limited | Wholesale & retail | 2019 |
| Dragon Thistle Brewing Company Limited | Manufacturing | 2020 |
| Dragonseight Ltd | Transport & logistics | 2016 |
| DRAIN4SURE Limited | Construction | 2021 |
| Dream Believe Achieve Academy Ltd | Arts & recreation | 2024 |
| Dream Dessertz Ltd | Wholesale & retail | 2025 |
| Dreghorn Eatery Ltd | Hospitality | 2025 |
| DRF Solutions Limited | Professional & scientific | 2025 |
| Drilling Environmental Solutions Ltd | Professional & scientific | 2017 |
| Drip TIE Ltd | Wholesale & retail | 2025 |
| Driveline Diagnostics Ltd | Professional & scientific | 2009 |
| DRM Investments Scotland Limited | Financial services | 2018 |
| DRM Property Limited | Construction | 2016 |
| DRM Staging Limited | Construction | 2020 |
| Droyx Ltd | Manufacturing | 2025 |
| Drums N Roses (Scotland) Ltd | Arts & recreation | 2019 |
| Drums N Roses Entertainment Ltd | Arts & recreation | 2020 |
| Drycoat Garden Ltd | Information & communication | 2022 |
| DS Mechanical Solutions Ltd | Manufacturing | 2023 |
| DS Removals Ltd | Extraterritorial | 2015 |
| DSS Care Ltd | Health & social care | 2025 |
| Dtektr Ltd | Administrative & support | 2024 |
| DTM Home Furnishings Limited | Wholesale & retail | 2018 |
| Duffy Diagnostics Ltd | Wholesale & retail | 2024 |
| Dugieosa Care Company Ltd | Health & social care | 2024 |
| Duha Foods Limited | Wholesale & retail | 2022 |
| Dumbarton Precision Engineering (Scotland) Ltd | Professional & scientific | 2014 |
| DUN Security Services Ltd | Administrative & support | 2024 |
| Dunadd Asset Managers Limited | Financial services | 2026 |
| Dunan Financial Ltd | Financial services | 2024 |
| Dunbar Brown Group Ltd | Administrative & support | 2023 |
| Dunbar RAE & Rose Ltd | Real estate | 2022 |
| Dunnet & Semple Contracts Glasgow Limited | Construction | 2025 |
| Dunross Properties Limited | Professional & scientific | 1993 |
| Dvbh Ltd | Real estate | 2022 |
| DW Contract Services Limited | Manufacturing | 2020 |
| Dynamic Tiling Ltd | Construction | 2015 |
| Dyno Heating (Scotland) Limited | Professional & scientific | 2014 |
| Dzcm Limited | Wholesale & retail | 2017 |
| E Macnaughton Consulting Ltd | Professional & scientific | 2024 |
| E-Gas Service Ltd | Energy | 2025 |
| E-Qualify Limited | Education | 2019 |
| E.S. PEER2PEER CIC | Health & social care | 2025 |
| E2 Solutions International Limited | Other services | 2017 |
| EAB Financial Services Ltd | Financial services | 2024 |
| Eadie Properties Ltd | Real estate | 2025 |
| Eager Cleavers Ltd | Wholesale & retail | 2023 |
| Eanna Press Limited | Information & communication | 2022 |
| Earbsa Professional Services Limited | Administrative & support | 2012 |
| Earls Paisley Ltd | Hospitality | 2026 |
| East Kilbride (Industrial Plastics) Limited | Manufacturing | 1987 |
| East Kitchen Limited | Hospitality | 2021 |
| East Star Paisley Ltd | Hospitality | 2026 |
| East West Cash and Carry Ltd | Wholesale & retail | 2022 |
| East West Chauffeur Drive Ltd | Transport & logistics | 2019 |
| Easterhouse Sun Ltd | Arts & recreation | 2011 |
| Eastern Delight Scots Limited | Hospitality | 2024 |
| Eastfullwood Properties Ltd | Real estate | 2015 |
| Eastknowe Developments Ltd | Construction | 2024 |
| Eastwood Properties (Scotland) Ltd | Real estate | 2018 |
| Easy Lets 4 U Limited | Real estate | 2011 |
| Easy Sales 4 U Limited | Real estate | 2016 |
| Easy Vehicle Finance Limited | Extraterritorial | 2012 |
| Easybuild Construction Limited | Construction | 2015 |
| Easydone Ltd | Real estate | 2026 |
| Easytec Group Ltd | Information & communication | 2018 |
| Eazee Street Limited | Construction | 2022 |
| EB Clean Services Limited | Administrative & support | 2025 |
| Eco Local Limited | Wholesale & retail | 2025 |
| Eco Specialists Scotland Ltd | Professional & scientific | 2023 |
| Ecofrost Hvac Ltd | Manufacturing | 2025 |
| Ecom New Horizons Ltd | Wholesale & retail | 2023 |
| Econodrone Ltd | Construction | 2018 |
| Ecoserv Limited | Construction | 2024 |
| ECP One Ltd | Real estate | 2026 |
| Edkr Ltd | Hospitality | 2021 |
| Educational Psychology Consultants Ltd | Education | 2023 |
| Eeleme Ltd | Real estate | 2024 |
| EFC Scotland Ltd | Agriculture & fishing | 2013 |
| Effective Creative Management Limited | Administrative & support | 2016 |
| EFL Solutions Ltd | Professional & scientific | 2010 |
| Ehho Ltd | Hospitality | 2025 |
| Eight Paisley Ltd | Hospitality | 2025 |
| Ejay Tearooms Ltd | Hospitality | 2018 |
| EJS Transport Ltd | Transport & logistics | 2024 |
| EKO Blast Ltd | Construction | 2026 |
| EL Bridge Scheme LP | | 2010 |
| EL Bridge Street (1 & 2) Limited | Real estate | 2022 |
| ELB Care Ltd | Other services | 2017 |
| Electrical Power Consultants Ltd | Construction | 2024 |