| Enhance Property Investment Company Limited | Real estate | 2024 |
| Enima Solutions Ltd | Wholesale & retail | 2018 |
| Enistic Limited | Professional & scientific | 2009 |
| Enlightenup Ltd | Arts & recreation | 2019 |
| ENN Energy Technology UK Ltd | Professional & scientific | 2025 |
| Ensou Ltd | Agriculture & fishing | 2018 |
| Ensurve Ltd | Professional & scientific | 2021 |
| Enthuse Transformations Ltd | Professional & scientific | 2016 |
| Entourage Mediashots Ltd | Information & communication | 2024 |
| Entropy Advisors Ltd | Professional & scientific | 2012 |
| Envalior UK Ltd. | Wholesale & retail | 2022 |
| Envecture Ltd | Professional & scientific | 2023 |
| Enviro-Spec UK Ltd | Construction | 2022 |
| Envision Architecture Limited | Professional & scientific | 2006 |
| Enzbond Limited | Professional & scientific | 2016 |
| Enzyme Supplies Limited | Manufacturing | 2005 |
| EOF Hackspace CIC | Professional & scientific | 2018 |
| Epeius Property Limited | Real estate | 2022 |
| Epeius Realty Limited | Construction | 2019 |
| Ephemerides Limited | Health & social care | 2016 |
| Epistal Ltd | Information & communication | 2026 |
| EPK Quality Builders Limited | Manufacturing | 2025 |
| Epoc Science Ltd | Professional & scientific | 2025 |
| Eptiva Therapeutics Ltd | Professional & scientific | 2020 |
| Equestrian Market Research Ltd | Professional & scientific | 2025 |
| Equilibrium Oxford Ltd | Health & social care | 2014 |
| Eqursus Limited | Professional & scientific | 2025 |
| ER274 Limited | Mining & quarrying | 2025 |
| ERA Education Office Ltd | Education | 2022 |
| ERA Oxford Limited | Hospitality | 2018 |
| Eradikil UK Ltd | Professional & scientific | 2023 |
| Erchonia Lasers Ltd | Administrative & support | 2013 |
| Eresia Limited | Extraterritorial | 2005 |
| Eridu Technologies Ltd | Information & communication | 2025 |
| Ernandes Sabino Ltd | Transport & logistics | 2026 |
| Ernest G Hart Limited | Administrative & support | 2002 |
| ERP Today Magazine Ltd | Information & communication | 2018 |
| Erpaccountancy Ltd | Professional & scientific | 2010 |
| Escape Imports Ltd | Wholesale & retail | 2018 |
| Esferas Technology Ltd | Information & communication | 2010 |
| Esh&Esh Groups Limited | Wholesale & retail | 2024 |
| Esia-Consult Limited | Professional & scientific | 2013 |
| Eskdale Services Limited | Wholesale & retail | 2007 |
| ESL (Events) Ltd | Hospitality | 2019 |
| Esme Vack Limited | Wholesale & retail | 2016 |
| Esotechnic Ltd | Professional & scientific | 2012 |
| Esquire Estates Oxford Ltd | Real estate | 2020 |
| Essence by Naura Ltd | Wholesale & retail | 2025 |
| Essenjay Associates Limited | Professional & scientific | 1996 |
| Essentials Ltd | Wholesale & retail | 2004 |
| Essentico Limited | Wholesale & retail | 2025 |
| Essquisse Ltd | Financial services | 2015 |
| Established Since 1654 Ltd | Hospitality | 2026 |
| Estelle Global Services Ltd | Hospitality | 2026 |
| Estelle Park Limited | Real estate | 2026 |
| Estuary Limited | Wholesale & retail | 2024 |
| ET AL Design Consultants Ltd | Professional & scientific | 2009 |
| ETF Strategy Limited | Information & communication | 2011 |
| Ethanjosh Ltd | Administrative & support | 2012 |
| Ethco Group Limited | Information & communication | 2021 |
| Ethex Investment Club Ltd | Financial services | 2010 |
| Ethical Data Initiative CIC | Professional & scientific | 2022 |
| Ethical Media Sales & Marketing Ltd | Professional & scientific | 2013 |
| Ethicore Limited | Health & social care | 2008 |
| Ethos Hosts Limited | Real estate | 2024 |
| Ethos Hotels Limited | Hospitality | 2007 |
| Ethos House Ltd | Real estate | 2023 |
| Ethospitality Ltd | Real estate | 2023 |
| Ethsun Executive Education Oxford Ltd | Education | 2023 |
| ETL Framework Ltd | Other services | 2010 |
| Eton Antiques Limited | Wholesale & retail | 2012 |
| Ettington Black Limited | Professional & scientific | 2002 |
| Euforic Services Limited | Information & communication | 2009 |
| Eugene Mcnally MSK Imaging Ltd | Health & social care | 2024 |
| Eulas Ltd | Hospitality | 2018 |
| Euphrosyne Investment Limited | Financial services | 2022 |
| Eurasia Educational Dynamics Ltd. | Education | 2017 |
| Euro Mini Market Nuneaton Ltd | Wholesale & retail | 2026 |
| Euro Supermarket Oxford Ltd | Wholesale & retail | 2018 |
| Eurointelligence Limited | Professional & scientific | 2006 |
| Europa Facilities Limited | Extraterritorial | 2001 |
| Europalaw Barristers Limited | Professional & scientific | 2019 |
| Europe Business Assembly Limited | Real estate | 2000 |
| European Centre for Journalism and Trauma Ltd | Information & communication | 2006 |
| European Cultural Programmes Limited | Arts & recreation | 2022 |
| European Event Organisation Ltd | Arts & recreation | 1999 |
| European Instruments Limited | Other services | 1975 |
| European News Academy Limited | Administrative & support | 2020 |
| Euroz Limited | Professional & scientific | 2006 |
| EVA Baron Ltd | Professional & scientific | 2018 |
| Evans Exports Ltd | Wholesale & retail | 2017 |
| Evelynparlour and Event Limited | Other services | 2020 |
| Evenlode Estates Limited | Professional & scientific | 1998 |
| Evenlode Properties Limited | Professional & scientific | 2005 |
| Event 53 Ltd | Administrative & support | 2024 |
| Event Clever Ltd | Hospitality | 2023 |
| Event Organiser Ltd | Other services | 2008 |
| Eventopia Ltd | Arts & recreation | 2025 |
| Ever Properties Ltd | Real estate | 2022 |
| Everblue Ltd | Wholesale & retail | 2020 |