| Crown Catering Services Limited | Hospitality | 2023 |
| Crystal Clear Management Solutions Ltd | Professional & scientific | 2008 |
| CS Systems (UK) Limited | Administrative & support | 2009 |
| CS UK Group Limited | Financial services | 2025 |
| Csja Services Limited | Manufacturing | 2024 |
| CSL Executive Search Limited | Administrative & support | 2023 |
| Cube Developments (Yorkshire) Limited | Other services | 2015 |
| Cura Technology Ltd | Information & communication | 2023 |
| Cwmakeadifference Limited | Professional & scientific | 2007 |
| Czujko Ltd | Real estate | 2018 |
| D Marshall Joinery Ltd | Construction | 2018 |
| D. Bourne and SON Ltd | Construction | 2019 |
| DAB Medical Services Ltd | Health & social care | 2024 |
| DAE Properties Limited | Real estate | 2013 |
| Dalesway Paving Ltd | Construction | 2020 |
| Dalesway Property & Storage Ltd | Real estate | 2021 |
| Dasulin Limited | Real estate | 1962 |
| Data Insight Training Limited | Information & communication | 2017 |
| Data Protection Assist Limited | Professional & scientific | 2025 |
| Data Scientists Limited | Information & communication | 2014 |
| Datamyst Ltd | Professional & scientific | 2023 |
| Dataphiles Limited | Information & communication | 2002 |
| Datech Management Solutions Limited | Professional & scientific | 2001 |
| David Joy | Financial services | 2009 |
| David Locke & Co Ltd | Professional & scientific | 2019 |
| Davinder Madaher Ltd | Professional & scientific | 2018 |
| Dawkins Developments Ltd | Real estate | 2026 |
| DC Air Conditioning Ltd | Energy | 2017 |
| Dcms Lawson Ltd | Financial services | 2022 |
| Denton Cars (Otley) Limited | Wholesale & retail | 2017 |
| Denton Cars (Skipton) Limited | Wholesale & retail | 2022 |
| Derailleurs Consulting Limited | Information & communication | 2025 |
| Design-Air Limited | Energy | 2013 |
| Designed and Directed Ltd | Professional & scientific | 2017 |
| Developing Strengths Ltd | Health & social care | 2018 |
| Diane Almond Marketing & PR Services Limited | Professional & scientific | 2008 |
| Digby Morton Ltd | Other services | 2013 |
| DIGITAL-3A Ltd | Information & communication | 2024 |
| Diller's 4 X 4S Limited | Wholesale & retail | 2010 |
| Dinaco Ltd | Financial services | 2025 |
| Discovery Centre Ltd | Wholesale & retail | 2024 |
| DJ Stock Limited | Manufacturing | 2016 |
| Djgp Holdings Ltd | Financial services | 2025 |
| DLS Management Ltd | Professional & scientific | 2022 |
| Dmpl Ltd | Construction | 2025 |
| DMS Properties (UK) Limited | Construction | 2011 |
| DNA Audio Ltd | Wholesale & retail | 2018 |
| Dobson Properties Ltd | Real estate | 2023 |
| Dodgson Meares Civils Ltd | Construction | 2020 |
| Dogtopia K9 Adventures Ltd | Other services | 2025 |
| Dottie About Dog Ltd | Other services | 2019 |
| Douglas Tonks Ltd | Information & communication | 2010 |
| Dowgill.Samuels & Associates Limited | Construction | 2026 |
| Dowson Joinery & Construction Ltd | Construction | 2026 |
| Drive Card Europe Limited | Wholesale & retail | 2015 |
| Dryden Wilkinson Partnership Limited | Professional & scientific | 2017 |
| Dunrig Limited | Construction | 2010 |
| DW Emptor Limited | Administrative & support | 2015 |
| E. Percy Limited | Construction | 1982 |
| Eboracum Surgery Limited | Health & social care | 2020 |
| Eco Lighting Solutions UK Ltd | Construction | 2019 |
| Eden View Escapes Ltd | Real estate | 2024 |
| Education for Business Ltd | Education | 2014 |
| Edulink Recruitment Ltd | Administrative & support | 2026 |
| Edward Johnson (Otley) Limited | Wholesale & retail | 1964 |
| Edward Ramsden Limited | Professional & scientific | 2002 |
| EF Cleaning Services Limited | Other services | 2009 |
| Egorum Limited | Professional & scientific | 2012 |
| Ejays Coffee Company Ltd | Hospitality | 2026 |
| EJK Recruitment Ltd | Administrative & support | 2019 |
| Elam Properties Ltd | Real estate | 2002 |
| Elda Building and Joinery Ltd | Construction | 2024 |
| Elddis Developments Limited | Construction | 2017 |
| Elddis Limited | Real estate | 2024 |
| Elddis Vehicles Limited | Wholesale & retail | 2021 |
| Eleanor Charles Limited | Wholesale & retail | 2022 |
| Electric Press Productions Ltd | Arts & recreation | 2024 |
| Electrical Power Products Ltd | Administrative & support | 2024 |
| Electro Evolution Limited | Professional & scientific | 2011 |
| Elemental Capital Ltd | Financial services | 2014 |
| Elise Consultancy Limited | Information & communication | 2025 |
| Elite Interpreters and Translators Ltd | Professional & scientific | 2019 |
| Elite Source and Supply Ltd | Administrative & support | 2024 |
| Ellison Homes Yorkshire Ltd | Construction | 2020 |
| Elowen Construction Ltd | Construction | 2024 |
| Elowen Group Ltd | Real estate | 2024 |
| Emergency 247 Plumbing & GAS Ltd | Construction | 2024 |
| Emery Developments (Yorkshire) Ltd | Construction | 2012 |
| Eminent Flooring Ltd | Wholesale & retail | 2019 |
| Empatica Ltd | Professional & scientific | 2016 |
| Empeiros Ltd | Health & social care | 2022 |
| Endure Communications Ltd | Professional & scientific | 2018 |
| Energis Systems Ltd | Professional & scientific | 2018 |
| Engage Comms Limited | Professional & scientific | 2012 |
| Enlighten Analytics Limited | Professional & scientific | 2016 |
| Enzymatic Hydrolysis Ltd | Manufacturing | 2025 |
| Erinvale Holdings Limited | Real estate | 2024 |
| ES Accountancy Limited | Professional & scientific | 2013 |
| Estate Management 62 Limited | Households | 1999 |
| Etempa Solutions Limited | Information & communication | 2012 |