| Safe Innovations Limited | Professional & scientific | 2014 |
| Safeguarding Advisory Services Ltd | Health & social care | 2010 |
| Salubrity Healthcare Limited | Health & social care | 2020 |
| Sammys Food Hub Ltd | Hospitality | 2025 |
| Sancta Books Ltd | Wholesale & retail | 2023 |
| Sandhurst Park Flat Management Co. (2) Limited | Households | 1984 |
| Sandie Properties Limited | Real estate | 2025 |
| SAP Castleford 2 Ltd | Real estate | 2025 |
| SAP Castleford Ltd | Real estate | 2025 |
| Saturn Search Limited | Administrative & support | 2025 |
| Scallan Machine Works Limited | Manufacturing | 2018 |
| Scarrott Medical Services Limited | Health & social care | 2016 |
| Scent Stop Ltd | Wholesale & retail | 2024 |
| Schappe Building Management Company Limited | Real estate | 2009 |
| Schedule IT Ltd | Information & communication | 2010 |
| SDK Hire Limited | Arts & recreation | 2025 |
| Sea and Sand Ltd | Hospitality | 2021 |
| Seasons School of Performing Arts Ltd | Arts & recreation | 2024 |
| Sebecca Limited | Professional & scientific | 2018 |
| Secret Cleans Ltd | Hospitality | 2020 |
| Secret Garden Cafe Otley Limited | Hospitality | 2024 |
| Seen Graphics Ltd | Information & communication | 2016 |
| Sentiva Limited | Information & communication | 2006 |
| Serviceloo Limited | Administrative & support | 2018 |
| SES Home Developments Ltd | Construction | 2023 |
| SG Flight Services Limited | Transport & logistics | 2018 |
| SG Safety Group Ltd | Professional & scientific | 2013 |
| Shadow Brewing Ltd | Manufacturing | 2018 |
| Shape Architects Limited | Professional & scientific | 2007 |
| Sharp & Scarffe Limited | Construction | 1999 |
| Shaw Reconstruction Ltd | Construction | 2024 |
| Shed Load Limited | Wholesale & retail | 2017 |
| Shellstone Properties Ltd | Real estate | 2025 |
| Sherlock Industries Ltd | Transport & logistics | 2025 |
| Shipley Home Care Services Limited | Health & social care | 2024 |
| Shokai and the Boros Ltd | Transport & logistics | 2021 |
| Show ME the Money (UK) Limited | Administrative & support | 2009 |
| SHU Business Advisory Limited | Professional & scientific | 2017 |
| Simmer Events Kitchen Ltd | Hospitality | 2021 |
| Simpson Building & Joinery Limited | Construction | 2021 |
| Sinclair Burton Limited | Professional & scientific | 2007 |
| SINF13LD Limited | Arts & recreation | 2008 |
| SJB Telecoms Limited | Extraterritorial | 2012 |
| SJL Artist Limited | Arts & recreation | 2025 |
| Skin Renew Otley Ltd | Other services | 2018 |
| Slaney Properties Limited | Construction | 2019 |
| Smartrepairs (OM) Limited | Other services | 2021 |
| Smtm LLP | | 2019 |
| Smtm Sports Ltd | Administrative & support | 2025 |
| Snowdon Hair Ltd | Other services | 2025 |
| SOL3K Ltd | Information & communication | 2013 |
| SOLE2HEAL Limited | Health & social care | 2012 |
| Solo Hair Team Otley Ltd | Other services | 2010 |
| Solutions Page Limited | Professional & scientific | 2017 |
| Soma Wellness Otley Ltd | Education | 2019 |
| Soma Wellness Retreats Ltd | Other services | 2025 |
| Soma&Yoke CIC | Professional & scientific | 2024 |
| Southworth Quarry Limited | Mining & quarrying | 1992 |
| Sowdens Heating Limited | Construction | 2013 |
| Speed Academy Ltd | Arts & recreation | 2018 |
| Sprayko Limited | Construction | 2018 |
| Springwood Bathrooms Ltd | Construction | 2023 |
| SR Agencies Ltd | Other services | 2009 |
| SR Kitchens & Bathrooms Ltd | Construction | 2019 |
| SS Restaurant Ltd | Hospitality | 2025 |
| Stagenorth Ltd | Education | 2025 |
| Stairs Direct UK Ltd | Wholesale & retail | 2015 |
| Stalspan Ltd | Construction | 2026 |
| Stamptastic Ltd | Wholesale & retail | 2011 |
| Starbeing Publishing Limited | Information & communication | 2025 |
| Stead and Co (UK) Limited | Professional & scientific | 2015 |
| Steel Property Developments Limited | Real estate | 2006 |
| Stephen Daniels Building Ltd | Construction | 2022 |
| Still NOT Done Ltd | Health & social care | 2025 |
| Straight Code Ltd | Information & communication | 2022 |
| Stratus Ventures Ltd | Wholesale & retail | 2026 |
| Stuart Thompson Plant Hire Limited | Administrative & support | 1993 |
| Stump Furniture Limited | Manufacturing | 2011 |
| Superstat Limited | Other services | 1993 |
| Susan Bilton Ltd | Professional & scientific | 2011 |
| Swift Helicopter Services Limited | Wholesale & retail | 2009 |
| Sydney Goldwyn Ltd | Wholesale & retail | 2018 |
| Synario (Events & Venues) Limited | Professional & scientific | 2002 |
| Synnera Ltd | Information & communication | 2024 |
| Syntera Systems Limited | Wholesale & retail | 2025 |
| T Briscoe I R Limited | Arts & recreation | 2013 |
| T&C Financial Services Limited | Financial services | 2019 |
| T.O.P Nails Otley Ltd | Other services | 2022 |
| Tailored Dog Grooming Limited | Wholesale & retail | 2019 |
| Tallyme Ltd | Information & communication | 2023 |
| Tanerock Properties Limited | Real estate | 1987 |
| Target Dental Limited | Health & social care | 2016 |
| Target Tyres Limited | Wholesale & retail | 2014 |
| Tarn Moor Estate Limited | Real estate | 2022 |
| Tate Fuel Oils Limited | Wholesale & retail | 1977 |
| Tate Leasing Ltd | Financial services | 2025 |
| Tate Medical Engineering Limited | Wholesale & retail | 1999 |
| Tate Solar Ltd | Construction | 2022 |
| TCB (UK) Limited | Administrative & support | 2006 |
| Teaching Plus Limited | Education | 2015 |